[1]翟宇瑶,段万里,郭宝林△.HPLC法测定淫羊藿中木兰花碱、朝藿定C和淫羊藿苷的含量[J].西部中医药,2019,32(07):34-36.
　ZHAI Yuyao,DUAN Wanli,GUO Baolin.The Contents of Magnoflorine, Epimedin C and Icariin in Yinyanghuo Determined by HPLC Method[J].Western Journal of Traditional Chinese Medicine,2019,32(07):34-36.
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HPLC法测定淫羊藿中木兰花碱、朝藿定C和淫羊藿苷的含量
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
32
期数:
2019年07期
页码:
34-36
栏目:
出版日期:
2019-07-15

文章信息/Info

Title:
The Contents of Magnoflorine, Epimedin C and Icariin in Yinyanghuo Determined by HPLC Method
文章编号:
1004-6852(2019)07-0034-03
作者:
翟宇瑶1，2，段万里3，郭宝林2△
1 西安市第四医院药剂科，陕西 西安 710004； 2 中国医学科学院北京协和医学院药用植物研究所；3 陕西省人民医院泌尿外科
Author(s):
ZHAI Yuyao1,2, DUAN Wanli 3, GUO Baolin2△
1 Pharmacy Department, Xi′an No.4 Hospital, Xi′an 710004, China;2 The Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College;3 Department of Urinary Surgery, Shaanxi Provincial People′s Hospital
关键词:
淫羊藿; 木兰花碱; 朝藿定C; 淫羊藿苷; HPLC
Keywords:
Yinyanghuo;  magnoflorine;  epimedin C;  icariin;  HPLC
分类号:
R284.2
文献标志码:
A
摘要:
目的：建立同时测定淫羊藿中木兰花碱、朝藿定C和淫羊藿苷含量的HPLC方法，研究淫羊藿(Epimedium brevicornu Maxim.)因产地不同带来的质量差异。方法：Venusil C18 MP色谱柱(250 mm×4.6 mm,5 μm)，乙腈-1%KH2PO4梯度洗脱；流速1 mL/min。结果：木兰花碱、朝藿定C和淫羊藿苷分别在4.0～52.0,6.4～206.0,7.0～242.0 μg/mL范围内线性关系良好；回收率分别为97.66%、101.10%、96.76%，RSD分别为2.14%、2.20%、1.58%。结论：本方法适用于同时测定淫羊藿中生物碱和黄酮两种不同类型的成分；不同分布地区淫羊藿中朝藿定C、淫羊藿苷均呈现较大的变化，木兰花碱含量变化大，不适于作为质控成分。
Abstract:
Objective: To establish a HPLC method for determining the contents of magnoflorine, epimedin C and icariin in Yinyanghuo (epimedii folium) simultaneously, and study the quality difference caused by the producing areas. Methods: Venusil C18 MP chromatographic column(250 mm×4.6 mm, 5 μm), acetonitrile-1% KH2PO4 as gradient elution; flow rate was 1 mL/min. Results: Under the above chromatographic conditions, magnoflorine, epimedin C and icariin showed good linear relationship in the range between 4.0～52.0, 6.4～206.0 and 7.0～242.0 μg/mL; the recovery rates were 97.66%, 101.10% and 96.76%, RSD were 2.14%, 2.20% and 1.58% respectively. Conclusion: The method is suitable for simultaneous determination of alkaloid and flavone in Yinyanghuo; the contents of epimedin C and icariin show greater changes contained in Yinyanghuo in different distribution regions, the contents of magnoflorine vary, and it couldn't be taken as the ingredients for quality control.

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备注/Memo

备注/Memo:
收稿日期：2018-04-03*基金项目：国家自然科学基金项目(编号81241137)。作者简介：翟宇瑶(1988—)，女，硕士学位，主管药师。研究方向：临床药学及药物分析。△通讯作者：郭宝林(1965—)，女，博士学位，研究员。研究方向：药物分析与中药资源管理。
更新日期/Last Update: 2019-07-15