[1]张平,李冬华,王晓琳,等.不同产地及加工方法对党参饮片质量的影响[J].西部中医药,2019,32(08):20-24.
 ZHANG Ping,LI Donghua,WANG Xiaolin,et al.Effects of Different Processing Methods and Different Producing Areas on the Quality of Dangshen Decoction Pieces[J].Western Journal of Traditional Chinese Medicine,2019,32(08):20-24.
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不同产地及加工方法对党参饮片质量的影响
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
32
期数:
2019年08期
页码:
20-24
栏目:
方药·制剂工艺
出版日期:
2019-08-15

文章信息/Info

Title:
Effects of Different Processing Methods and Different Producing Areas on the Quality of Dangshen Decoction Pieces
文章编号:
1004-6852(2019)08-0020-05
作者:
张平12李冬华12王晓琳34张明童12马潇12宋平顺12杨静12
1 甘肃省药品检验研究院,甘肃 兰州 730070; 2 甘肃省中藏药检验检测工程技术实验室;3 甘肃省中医院; 4 甘肃省中医药研究院
Author(s):
ZHANG Ping 12 LI Donghua 12 WANG Xiaolin 34 ZHANG Mingtong 12 MA Xiao 12 SONG Pingshun 12 YANG Jing 12
1 Gansu Institute for Drug Control, Lanzhou 730070, China; 2 Gansu Province Engineering andTechnical Laboratory of Chinese and Tibetan Drugs Inspection and Testing;3 Gansu Provincial Hospital of Traditional Chinese Medicine; 4 Gansu Provincial Academy of Chinese Medicine
关键词:
不同产地加工方法党参炔苷党参多糖指纹图谱质量控制
Keywords:
different processing method and different producing areas codonopsis pilosula alkynyl glycoside codonopsis pilosula polysaccharide fingerprint quality control
分类号:
R282
文献标志码:
A
摘要:
目的:研究不同产地加工方法对党参饮片中党参炔苷、党参多糖、药典党参项下指标性成分的影响,并建立不同产地加工方法下党参指纹图谱,为党参不同产地加工方法质量控制及产地加工方法原理研究提供依据。方法:选用不同产地加工方式处理的党参样品,考察不同方法对党参饮片中党参炔苷含量,党参多糖含量等指标的影响,采用HPLC色谱法测定党参炔苷含量,采用用紫外-可见分光光度法测定党参多糖含量;采用HPLC-DAD检测法对10种不同产地加工方式处理的党参结,党参段进行指纹图谱研究,采用指纹图谱相似度软件进行分析。结果:党参炔苷进样量在0.33~6.55 μg范围内与峰面积呈良好的线性关系;党参多糖(以葡萄糖为对照)在0.294 8~2.528 2 mg/mL的范围内,浓度与吸光度值呈良好线性关系;以党参炔苷峰为参照峰,建立不同产地加工方法下党参HPLC-DAD指纹图谱,确认13个共有指纹峰,特征明显,结果可靠。党参经不同产地加工方法处理后品质亦有不同变化。结论:本实验所采用方法精密度、稳定性和重复性良好,符合含量测定及指纹图谱技术等要求,可作为合理选择党参产地加工方式的质量控制方法。
Abstract:
Objective: To provide the reference for the study on the principles and quality control for Dangshen processed by different methods from different producing areas by investigating the effects of the processing methods from different producing areas on Dangshen indicative components in pharmacopoeia, ginseng polysaccharide and codonopsis pilosula alkynyl glycoside contained in Dangshen, establishing the fingerprint of Dangshen processed by the methods from different producing areas. Methods: After processing the samples with different methods from different producing areas, the effects of different processing methods were surveyed on the contents of ginseng polysaccharide and codonopsis pilosula alkynyl glycoside contained in Dangshen, HPLC chromatographic method was adopted to determine the contents of codonopsis pilosula alkynyl glycoside, CAPCELL PAK C18-MG Ⅱ column (4.6 mm×250 mm, 5 μm), mobile phase was methanol: water (28:72), flow rate 1.0mL/min, column temperature 30℃, detection wavelength 267nm; ultraviolet-visible spectrophotometry was used to measure the contents of codonopsis pilosula polysaccharide; HPLC-DAD detection method was employed to study the fingerprint print of the knot and part of Dangshen processed by ten methods from different producing areas, and the fingerprint was analyzed using fingerprint similarity software. Results: Sample size of codonopsis pilosula alkynyl glycoside showed better linear relationship in the rang from 0.33 to 6.55 μg and peak area; when codonopsis pilosula polysaccharide (glucose as the control) was ranged from 0.294 8 to 2.528 2 mg/mL, the concentrations and absorbance value were in good linear relationship; by taking the peak of codonopsis pilosula alkynyl glycoside as the control, HPLC-DAD fingerprint of Dangshen was established after processed with different methods, finally 13 common fingerprint peaks were confirmed, and they presented the obvious characteristics and reliable results. The property of Dangshen has changed differently after processed with different methods of different producing areas. Conclusion: The method employed in the experiment shows better precision, stability and repeatability, meets the requirements of content determination and fingerprint technique, and it could be taken as the method of quality control for rationally choosing processing methods of Dangshen.

相似文献/References:

[1]张承军,王建良,李川国,等.甘肃产党参中党参炔苷含量的动态研究[J].西部中医药,2014,27(09):7.
[2]马琴国,安培坤,王引权△.不同采样期党参炔苷的含量动态变化分析*[J].西部中医药,2014,27(02):23.
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[3]李彩东,王信,潘新波,等.波长切换技术同时测定加味小柴胡汤胶囊中党参炔苷等4种成分[J].西部中医药,2017,30(01):26.
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备注/Memo

备注/Memo:
收稿日期:2018-05-07*基金项目:甘肃省科技重大专项计划项目(编号17ZD2FA009);甘肃省食品药品重点研发项目(编号2017GSFDA012)。作者简介:张平(1983—),男,主管中药师。研究方向:中药材鉴定与检验,中药材藏药质量标准控制和评价。
更新日期/Last Update: 2019-08-15