[1]刘永巧,魏颖,王扬,等.HPLC同时测定辅助降糖片中芦丁、异槲皮苷、槲皮素的含量[J].西部中医药,2020,33(02):22-25.[doi:10.12174/j.issn.1004-6852.2020.02.06]
　LIU Yongqiao,WEI Ying,WANG Yang,et al.Content Determination of Rutin, Isoquercitrin and Quercetin in Fuzhu Jiangtang Tablets by HPLC Simultaneously[J].Western Journal of Traditional Chinese Medicine,2020,33(02):22-25.[doi:10.12174/j.issn.1004-6852.2020.02.06]
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HPLC同时测定辅助降糖片中芦丁、异槲皮苷、槲皮素的含量
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
33
期数:
2020年02期
页码:
22-25
栏目:
出版日期:
2020-02-15

文章信息/Info

Title:
Content Determination of Rutin, Isoquercitrin and Quercetin in Fuzhu Jiangtang Tablets by HPLC Simultaneously
文章编号:
1004-6852(2020)02-0022-04
作者:
刘永巧，魏颖，王扬，孙巍巍，何乐毅凡，徐暾海，刘铜华
1 北京中医药大学中药学院，北京 100029； 2 中医养生学北京市重点实验室；3 中医养生学教育部重点实验室
Author(s):
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1School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100029, China;2Beijing Municipality Health Cultivation Laboratory of Beijing University of Chinese Medicine;3Health Cultivation Laboratory of the Ministry of Education of Beijing University of Chinese Medicine
关键词:
辅助降糖片; 高效液相色谱法; 芦丁; 异槲皮苷; 槲皮素
Keywords:
Fuzhu Jiangtang tablets;  HPLC;  rutin;  isoquercitrin;  quercetin
分类号:
R285.5
DOI:
10.12174/j.issn.1004-6852.2020.02.06
文献标志码:
A
摘要:
目的：建立同时测定辅助降糖片中芦丁、异槲皮苷、槲皮素含量的方法。方法：采用高效液相色谱法。色谱柱：Thermo syncronis C18(250 mm×4.6 mm，5 μm)。流动相：乙腈(A)-0.1%甲酸水(B)。梯度洗脱：0～5分钟，12%(B)；5～15分钟，22%(B)；15～25分钟，28%(B)；25～30分钟，30%(B)；30～40分钟，35%(B)。流速：1 mL/min。柱温：35℃。检测波长：370 nm。进样量为20 μL。结果：芦丁、异槲皮苷、槲皮素的进样量分别在50～1000 mg/L(r1=0.998)、20～600 mg/L(r2=0.997)、10～200 mg/L(r3=0.998)范围内与峰面积积分值呈良好线性关系；平均回收率分别为102.62%、98.58%、100.31%。结论：该方法准确、可靠、重复性良好可用于辅助降糖片的质量控制。
Abstract:
Objective: To establish the methods of detecting the contents of rutin, isoquercitrin and quercetin contained in Fuzhu Jiangtang tablets simultaneously. Methods: HPLC method was used. Chromatographic column: Thermo syncronis C18 (250 mm×4.6 mm, 5 μm); mobile phase: acetonitrile (A)- 0.1% formic acid (B); gradient elution: 0 ~ 5mintues, 12%(B); 5 ~ 15minutes, 22%(B): 15 ~ 25 minutes, 28%(B); 25 ~ 30 minutes, 30%(B); 30~40minutes, 35%(B); flow rate: 1 mL/min; column temperature: 35℃; detection wavelength was 370 nm; sample size was 20 μL. Results: Rutin, isoquercitrin and quercetin presented good linear relationship when its sample size ranged from 50 to 1000 mg/L(r1=0.998), 20 to 600 mg/L(r2=0.997), and 10 to 200 mg/L(r3=0.998) and the integral of peak area, average recovery rates were 102.62%, 98.58% and 100.31%.Conclusion: The method, accurate, reliable and repeatable, could be used for quality control of Fuzhu Jiangtang tablets.

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备注/Memo

备注/Memo:
收稿日期：2019-03-26*基金项目：科学研究与研究生培养共建项目-成果转化与产业化项目。作者简介：刘永巧(1990—)，女，在读硕士研究生。研究方向：中药复方干预糖尿病的药效物质基础及作用机制研究。通讯作者：徐暾海(1969—)，男，博士学位，教授。研究方向：中药活性成分及质量控制。
更新日期/Last Update: 2020-02-15