蓝萼香茶菜总二萜对实验性兔心肌缺血相关蛋白的影响-《西部中医药》

文章信息/Info

Title:: Effects of Total Diterpenes of Cycalyx Herb on Rabbit Myocardial Ischemia/reperfusion Related Protein

Author(s):: LIU Mingjie; Inner Mongolia University for Nationalities, Tongliao 028000, China

Keywords:: myocardial ischemia/reperfusion injury; total diterpenes (TD) of cycalyx herb; apoptosis related protein; rabbit

摘要:: 目的探讨蓝萼香茶菜总二萜（total diterpenes of cycalyx herb，TD）对实验性兔心肌缺血/再灌注损伤相关蛋白的保护作用。方法将健康日本大耳白兔18只分为3组，A：假手术（Sham）组；B：缺血/再灌注（I/R）组；C：TD组。光镜和电镜下观察3组兔心肌细胞结构变化情况；检测再灌注180 min时3组兔血清丙二醛（MDA）、超氧化物歧化酶（SOD）及心肌MDA、SOD、谷胱甘肽过氧化物酶（GSH-Px）和Caspase 3活性变化情况；WB法检测3组兔心肌细胞凋亡蛋白Bcl-2和Bax的含量；评价TD预处理对在体兔心肌缺血/再灌注损伤（MI/RI）动物模型的保护作用。结果TD组与I/R组相比：血清MDA、心肌MDA和Caspase 3下降（P＜0.05），血清SOD、心肌SOD和GSH-Px增加（P＜0.05），Bax蛋白表达下降（P＜0.05），Bcl-2蛋白表达增加（P＜0.05）。结论TD能减少实验性兔MI/RI的促凋亡蛋白Bax的表达，增加抗凋亡蛋白Bcl-2的表达，提示该药对心肌细胞凋亡有保护作用。

Abstract:: ObjectiveTo survey the protective effects of total diterpenes (TD) of cycalyx herb on myocardial ischemia/reperfusion (I/R) related protein in rabbits. MethodsAll 18 rabbits were separated into three groups: A:sham operation group (Sham); B: ischemia/reperfusion(I/R) group; and C: TD group. The changes in myocardial cells structure of the rabbits in three groups were observed under light microscope and electrical microscope; the changes in MDA and SOD activity in the serum, MDA, SOD, GSH-Px and Caspase 3 activity in myocardial cells were measured after reperfusion for 180 minutes; the contents of myocardial cells apoptosis-related protein Bcl-2 and Bax in rabbit myocardial cells were detected using WB method; the protective effects of TD preconditioning on MI/RI rabbit model were assessed. ResultsWhen TD group was compared with I/R group: serum MDA, myocardial MDA and Caspase 3 reduced(P＜0.05), serum SOD, myocardial SOD and GSH-Px increased(P＜0.05), Bax protein expressions lowered(P＜0.05), Bcl-2 protein expression increased(P＜0.05). ConclusionTD could reduce apoptosis-related protein Bax expression and increase anti-apoptosis protein Bcl-2 expression, and it is suggested that TD has the protective effects on the apoptosis of myocardial cells.