[1]张建武,罗林,罗涛,等.柴胡皂苷d对兔离体十二指肠平滑肌收缩活动的影响[J].西部中医药,2021,34(11):46-49.[doi:10.12174/j.issn.2096-9600.2021.11.10]
 ZHANG Jianwu,LUO Lin,LUO Tao,et al.Influece of Saikosaponin d on the Contraction of Isolated Rabbit Duodenal Smooth Muscle[J].Western Journal of Traditional Chinese Medicine,2021,34(11):46-49.[doi:10.12174/j.issn.2096-9600.2021.11.10]
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柴胡皂苷d对兔离体十二指肠平滑肌收缩活动的影响
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
34
期数:
2021年11期
页码:
46-49
栏目:
药理研究
出版日期:
2021-11-15

文章信息/Info

Title:
Influece of Saikosaponin d on the Contraction of Isolated Rabbit Duodenal Smooth Muscle
作者:
张建武 罗林 罗涛 杨春艳 陈建华 邓艾佳 陈卫
川北医学院,四川 南充 637100
Author(s):
ZHANG Jianwu LUO Lin LUO Tao YANG Chunyan CHEN Jianhua DENG Aijia CHEN Wei
North Sichuan Medical University, Nanchong 637100, China
关键词:
十二指肠平滑肌收缩活动柴胡皂苷d
Keywords:
duodenal smooth musclecontactionsaikosaponin drabbit
分类号:
R291.2
DOI:
10.12174/j.issn.2096-9600.2021.11.10
文献标志码:
A
摘要:
目的研究柴胡皂苷d(Saikosaponin d,SSd)对兔离体十二指肠平滑肌收缩活动的影响及其作用机制。 方法采用经典的家兔离体十二指肠平滑肌试验,应用BL-420F生物机能实验系统,以9、18、36 μmol/L浓度SSd溶液作用于兔离体十二指肠平滑肌,观察记录兔离体十二指肠平滑肌的平均收缩力及收缩频率,并应用工具药肌浆网Ryanodine受体阻断剂钌红(ruthenium red,RR)10 μmol/L、三磷酸肌醇(inositol triphosphate,IP3)受体阻断剂肝素(heparin,HP)50 mg/L和L型Ca2+通道拮抗剂维拉帕米(Verapamil,V)0.1 μmol/L研究SSd影响离体十二指肠平滑肌收缩活动的作用机制。 结果SSd可浓度依赖性增强兔离体十二指肠平滑肌自发性收缩张力(P<0.05),但对其收缩频率无明显影响(P>0.05)。维拉帕米可影响SSd增强兔离体十二指肠平滑肌平均收缩力的作用(P<0.01),而RR和HP对此无明显影响(P>0.05)。 结论SSd可浓度依赖性增强兔离体十二指肠平滑肌的平均收缩力,其作用机制可能与增强L型钙离子通道的活性从而促进外钙内流有关,而与肌浆网上IP3受体途径和Ryanodine受体途径介导的内钙释放无明显关联。
Abstract:
ObjectiveTo study the effects of saikosaponin d(SSd) on the contraction of isolated rabbit duodenal smooth muscle and its mechanism. MethodsClassical isolated rabbit duodenal smooth muscle test in vitro was adopted, BL-420F biological function experiment system was used, the average contractile force and contractile frequency of isolated rabbit duodenal smooth muscle were observed and recorded after acting on isolated rabbit duodenal smooth muscle with 9, 18 and 36 μmol/L of SSd solution, tool drug sarcoplasmic reticulum ryanodine receptor blocker RR, 10 μmol/L, IP3 receptor blocker HP 50 mg/L and L-type Ca2+ channel antagonist V, 0.1 μmol/L, were applied to survey the mechanism of SSd on the contractile activity of isolated rabbit duodenal smooth muscle. ResultsSSd could enhance spontaneous systolic tension of isolated rabbit duodenal smooth muscle in concentration dependent manner (P<0.05), while it showed no influence on its contractile frequency (P>0.05). V could affect the effect of SSd on the average contractility of isolated rabbit duodenal smooth muscle (P<0.01), while RR and HP showed no influence (P>0.05). ConclusionSSd could enhance spontaneous systolic tension of isolated rabbit duodenal smooth muscle in concentration dependent manner, and its mechanism might be related to enhancing the activity of L-type calcium channel and promoting external calcium influx, but it is not significantly related to the release of internal calcium mediated by IP3 receptor pathway and Ryanodine receptor pathway on sarcoplasmic reticulum.

相似文献/References:

[1]杨麒民,罗涛,李春臣,等.人参皂苷Rb1对兔离体肠平滑肌收缩活动的影响[J].西部中医药,2021,34(10):48.[doi:10.12174/j.issn.2096-9600.2021.10.11]
 YANG Qimin,LUO Tao,LI Chunchen,et al.Effects of Ginsenoside Rb1 on Isolated Rabbit Intestine Smooth Muscle Contraction[J].Western Journal of Traditional Chinese Medicine,2021,34(11):48.[doi:10.12174/j.issn.2096-9600.2021.10.11]

备注/Memo

备注/Memo:
张建武(1980—),男,硕士学位,副教授。研究方向:平滑肌活性和肿瘤新药药理研究。四川省科技厅重点项目(2016JY0032);四川省大学生创新训练项目(201810634169、S202010634182)。
更新日期/Last Update: 2022-08-09