甘草查尔酮胶囊质量标准研究-《西部中医药》

文章信息/Info

作者:: 孙宇¹, 贾智超², 张娟¹, 徐晓琴¹, 卿德刚¹; 1.新疆维吾尔自治区中药民族药研究所，新疆乌鲁木齐 830002
2.乌鲁木齐市中医医院，新疆乌鲁木齐 830000

Author(s):: SUN Yu¹, JIA Zhichao², ZHANG Juan¹, XU Xiaoqin¹, QING Degang¹; 1.Herb and Ethnic Drugs Institute of Xinjiang Uygur Autonomous Region, Urumqi 830002, China
2.Urumqi Hospital of Traditional Chinese Medicine, Urumqi 830000, China

摘要:: 目的建立甘草查尔酮胶囊的质量标准。方法采用薄层色谱法对甘草查尔酮胶囊进行薄层鉴别；采用高效液相色谱法测定甘草查尔酮A的含量。色谱柱Waters XTerra RP C₁₈（250 mm×4.6 mm，5 μm）；流动相乙腈-1%磷酸溶液（58∶42）；体积流量1.0 mL/min；检测波长310 nm；柱温25 ℃。结果甘草查尔酮胶囊薄层色谱图斑点清晰，阴性无干扰；甘草查尔酮A在0.05125～0.5125 mg内呈良好的线性关系（r=0.999 9），平均回收率为100.76%，RSD为1.62%（n=6）。结论本研究建立的甘草查尔酮胶囊的薄层鉴别方法及甘草查尔酮A含量测定方法，增强了该制剂的可控性，为医院制剂或新药研发提供了理论数据。

Abstract:: ObjectiveTo establish the quality standard of licorice chalcone capsules. MethodsTLC was adopted to perform thin-layer identification of licorice chalcone capsules, HPLC was used to detect the contents of licochalcone A. Chromatographic column Waters XTerra RP C₁₈ (250 mm×4.6 mm, 5 μm); mobile phase acetonitrile-1% phosphoric acid solution (58:42); volume flow: 1.0 mL/min; detection wavelength: 310 nm; column temperature: 25℃. ResultsTLC of licorice chalcone capsules presented clear spots, and it was negative without interference; licochalcone A showed good linear relationship between 0.05125 and 0.5125 mg (r=0.999 9), average recovery rate was 100.76%, RSD was 1.62% (n=6). ConclusionTLC of licorice chalcone capsules and content determination method of licochalcone A established in the study could enhance the controllability of the preparations, which could provide theoretical data for scientific research and development of the hospital preparations and new drugs.