[1]马琼,闫龙腾,赵茜,等.砂仁对反流性食管炎大鼠食管黏膜的保护作用[J].西部中医药,2023,36(10):1-6.[doi:10.12174/j.issn.2096-9600.2023.10.01]
　MA Qiong,YAN Longteng,ZHAO Xi,et al.The Protective Effects of Sharen on Esophageal Mucosa in Rats with Reflux Esophagitis[J].Western Journal of Traditional Chinese Medicine,2023,36(10):1-6.[doi:10.12174/j.issn.2096-9600.2023.10.01]
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砂仁对反流性食管炎大鼠食管黏膜的保护作用
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
36
期数:
2023年10期
页码:
1-6
栏目:
出版日期:
2023-10-15

文章信息/Info

Title:
The Protective Effects of Sharen on Esophageal Mucosa in Rats with Reflux Esophagitis
作者:
马琼1,2, 闫龙腾1,2, 赵茜1,2, 胡冬雄1
1.云南中医药大学基础医学院，云南 昆明 650504
2.云南省高校中医证候微观辨证重点实验室，云南 昆明 650504
Author(s):
MA Qiong1,2, YAN Longteng1,2, ZHAO Xi1,2, HU Dongxiong1
1.College of Basic Medicine, Yunnan University of Traditional Chinese Medicine, Kunming 650504, China
2.Key Laboratory of Microscopic Syndrome Differentiation of Traditional Chinese Medicine in Universities of Yunnan Province, Kunming 650504, China
关键词:
食管炎; 反流性; 砂仁; 氧化应激; 血管活性肠肽; P物质; 一氧化氮合酶2; 大鼠; 动物实验
Keywords:
esophagitis;  reflux; oxidative stress; VIP; substance P; NOS2; rats; animal experiments
分类号:
R975
DOI:
10.12174/j.issn.2096-9600.2023.10.01
文献标志码:
A
摘要:
目的探讨砂仁对反流性食管炎（reflux esophagitis，RE）大鼠食管黏膜是否有保护作用，并从一氧化氮合酶2（nitric oxide synthase2，NOS2）蛋白途径研究其作用机制，为砂仁的开发和RE的治疗提供理论基础。 方法选取100只清洁级雄性SD大鼠，采用“4.2 mm幽门夹+胃底2/3结扎术”构建RE大鼠模型，造模成功后随机选取50只，按照随机数字法分为模型组，奥美拉唑组，砂仁低、中、高剂量组，每组10只。另外选取10只为假手术组，治疗4周后收集大鼠食管组织与血清，HE染色观察组织病理学，试剂盒检测血清氧化和抗氧化指标超氧化物歧化酶（superoxide dismutase，SOD）、丙二醛（malondialdehyde，MDA）和一氧化氮（nitric Oxide，NO）含量，同时采用PCR和Western Blotting检测食管组织中血管活性肠肽（vasoactive intestinal peptide，VIP）、P物质（substance P，SP）和NOS2的mRNA及蛋白的表达特点。 结果假手术组食管黏膜光滑、结构完整，没有明显炎症反应和水肿表现；模型组可见有明显食管黏膜增厚，同时角质化层明显，局部黏膜呈乳头样凸起；各治疗组及奥美拉唑组相较于模型组均有明显改善，且随着剂量的升高，食管病理改善效果越明显；与奥美拉唑组相比，砂仁低、中剂量组改善食管病理学情况没有奥美拉唑组显著（P＞0.05），而高剂量组与奥美拉唑组疗效相似（P＞0.05）。与假手术组相比，模型组SOD明显降低（P＞0.05），MDA和NO明显升高（P＞0.05），砂仁治疗后MDA和NO明显降低（P＞0.05），而SOD则明显升高（P＞0.05）；与低剂量相比，砂仁高剂量组SOD明显升高、MDA和NO显著降低（P＞0.05），而与中剂量相比差异无统计学意义（P＞0.05）；与奥美拉唑组相比，砂仁低、中剂量组SOD水平降低、MDA和NO水平升高（P＞0.05）；而高剂量组SOD水平、MDA和NO水平，与奥美拉唑组比较差异无统计学意义（P＞0.05）。与假手术组相比，模型组大鼠VIP蛋白及mRNA表达明显降低（P＞0.05），SP和NOS2蛋白及mRNA表达明显升高（P＞0.05），而各治疗组相较于模型组，VIP则明显升高（P＞0.05），SP和NOS2明显降低（P＞0.05）。 结论砂仁对大鼠反流性食管黏膜损伤具有明显的保护作用，其作用机理可能与其能够下调VIP和NOS2表达水平、上调SP表达水平来调节胃肠运动，调节MTL、SOD及MDA的分泌进而提高抗氧化能力有关。
Abstract:
ObjectiveTo survey whether Sharen (amomi fructus) has the protective effects on the esophageal mucosa of rats with reflux esophagitis (RE), and study its mechanism from NOS2 protein pathway, in order to provide a theoretical basis for the development of Sharen and the treatment of RE. MethodsAll 100 clean grade male SD rats were chosen to establish RE rat models using "4.2mm pyloric clip+gastric fundus 2/3 ligation", after successful modeling, 50 rats were randomly selected, and allocated to the model group, omeprazole group, low, moderate and high dose groups of Sharen using random number table method, with ten rats in each group. In addition, ten rats were chosen as sham operation group, after four weeks of the treatment, to collect esophageal tissue and blood serum, HE staining was used to observe the histopathology, to detect the contents of SOD, MDA and NO using kit, meanwhile, PCR and Western Blotting were adopted to measure the expressions of VIP, substance P and NOS2 mRNA and protein in esophageal tissue. ResultsIn the sham operation group, the esophageal mucosa was smooth and intact, without obvious inflammation and edema; the model group showed obvious thickening of the esophageal mucosa, while the stratum corneum was obvious, the local mucosa was nipple-like protrusions; all the treatment groups and omeprazole group presented the noticeable improvements than the model group, and the improvements of esophageal pathology were more obvious with the increase of dose; compared with omeprazole group, the improvements in esophageal pathology in low and moderate dose groups of Sharen were not significant in the omeprazole group (P>0.05), while high dose group showed similar clinical effects to omeprazole group (P>0.05). Compared with sham operation group, the level of SOD lowered apparently in the model group (P>0.05), the levels of MDA and NO raised clearly (P>0.05), after treating with Sharen, the levels of MDA and NO reduced obviously (P>0.05), while the level of SOD lifted remarkably (P>0.05), compared with low dose group, the level of SOD hoisted while the levels of MDA and NO reduced apparently in high dose group of Sharen (P>0.05), however, the difference had no statistical meaning compared with medium dose group (P>0.05); compared with omeprazole group, the level of SOD lowered while the levels of MDA and NO increased in low and medium dose groups of Sharen (P>0.05); the difference had no statistical meaning when high dose group was compared with omeprazole group in the levels of SOD, MDA and NO (P>0.05). Compared with sham operation group, the expressions of VIP protein and mRNA went down obviously in the model group (P>0.05), the expressions of SP, NOS2 protein and mRNA increased apparently (P>0.05), when all the treatment groups were compared with the model group, the levels of VIP raised obviously (P>0.05), while the levels of SP and NOS2 lowered clearly (P>0.05). ConclusionSharen has an obvious protective effects on esophageal mucosal injury in RE rats, and its mechanism might be related to adjusting gastrointestinal motility, regulating the secretion of MTL, SOD and MDA, and thus enhancing the antioxidant capacity through down regulating the expressions of VIP and NOS2, up regulating the expressions of SP.

相似文献/References:

[1]姚柳伊,田旭东,舒劲△.近5年反流性食管炎中医研究进展[J].西部中医药,2014,27(04):140.
　YAO Liuyi,TIAN Xudong,SHU Jin.TCM Study of Reflux Esophagitis in Recent Five Years[J].Western Journal of Traditional Chinese Medicine,2014,27(10):140.
[2]刘明儒.半夏泻心汤治疗反流性食管炎50例[J].西部中医药,2014,27(10):98.
[3]王红梅,杨小茜,刘晶,等.健脾降逆法治疗脾虚型反流性食管炎疗效评价[J].西部中医药,2019,32(08):85.
　WANG Hongmei,YANG Xiaoxi,LIU Jing,et al.Clinical Evaluation on Spleen-invigorating Counterflow-descending Method in the Treatment for Reflux Esophagitis of Spleen-deficiency Pattern[J].Western Journal of Traditional Chinese Medicine,2019,32(10):85.

备注/Memo

备注/Memo:
马琼（1994—），女，硕士学位，医师。研究方向：非酒精性脂肪肝病的中医药治疗。云南省科学技术厅-云南中医药大学应用基础研究联合专项［2019FF002（-084）］。
更新日期/Last Update: 2023-10-15