[1]江四华,代卓青,刘田,等.四逆汤通过自噬途径对脓毒症大鼠左心室功能的影响[J].西部中医药,2023,36(11):19-23.[doi:10.12174/j.issn.2096-9600.2023.11.04]
　JIANG Sihua,DAI Zhuoqing,LIU Tian,et al.Effects of Sini Tang on Left Ventricular Function in Sepsis Rats via Autophagy[J].Western Journal of Traditional Chinese Medicine,2023,36(11):19-23.[doi:10.12174/j.issn.2096-9600.2023.11.04]
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四逆汤通过自噬途径对脓毒症大鼠左心室功能的影响
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
36
期数:
2023年11期
页码:
19-23
栏目:
出版日期:
2023-11-15

文章信息/Info

Title:
Effects of Sini Tang on Left Ventricular Function in Sepsis Rats via Autophagy
作者:
江四华1, 代卓青1, 刘田1, 宋国林2
1.贵州中医药大学，贵州 贵阳 550000
2.贵州中医药大学第二附属医院，贵州 贵阳 550003
Author(s):
JIANG Sihua1, DAI Zhuoqing1, LIU Tian1, SONG Guolin2
1.Guizhou University of Traditional Chinese Medicine, Guiyang 550000, China
2.The Second Affiliated Hospital of Guizhou University of Traditional Chinese Medicine, Guiyang 550003, China
关键词:
脓毒症; 自噬; 左心室功能; 四逆汤; 大鼠
Keywords:
sepsis; autophagy; left ventricular function; rats
分类号:
R285.5
DOI:
10.12174/j.issn.2096-9600.2023.11.04
文献标志码:
A
摘要:
目的探讨四逆汤通过自噬途径对脓毒症大鼠左心室功能的调控作用。 方法将150只雌性SD大鼠随机选取30只作为空白组（8 h、16 h、24 h各10只），其余120只按盲肠结扎穿刺法造模，将造模成功的90只大鼠按随机数字表方法分为实验组、对照组、阻断组各30只，每组再分为8、16、24 h组各10只。实验组灌胃四逆汤，对照组与空白组灌胃生理盐水，阻断组灌胃四逆汤＋3-MA（6-氨基-3-甲基嘌呤）注射阻断，观察各组大鼠8、16、24 h心脏超声左心室收缩末期容积、左心室舒张末期容积、心输出量、左心室射血分数；并通过WB检测大鼠心肌自噬蛋白P62、LC3-I、LC3-Ⅱ水平。 结果1）干预8、16 h实验组大鼠左心室功能优于空白组、对照组、阻断阻（P＜0.05），对照组与阻断组比较差异无统计学意义（P＞0.05），空白组左心室功能优于对照组和阻断组（P＜0.05）；干预24 h大鼠左心室功能实验组、对照组、阻断组两两比较差异无统计学意义（P＞0.05）。2）干预8、16 h实验组大鼠自噬蛋白表达强于对照组、阻断组（P＜0.05），对照组与阻断组比较差异无统计学意义（P＞0.05）；干预24 h，大鼠自噬蛋白表达实验组、对照组、阻断组两两比较差异无统计学意义（P＞0.05）。 结论四逆汤可能通过自噬途径改善脓毒症大鼠左心室功能。
Abstract:
ObjectiveTo discuss the regulatory effects of Sini Tang on left ventricular function in sepsis rats via autophagy. MethodsThirty rats were randomly chosen from 150 female SD rats as the blank group (ten rats at 8 h, 16 h and 24 h respectively), the remaining 120 were prepared into the models in light of cecal ligation puncture (CLP), 90 rats, successfully modelling, were divided into the experiment group, the control group and the blockade group, 30 cases in each group, and each group were subdivided into the groups of 8 h, 16 h and 24 h with ten in each. The experiment group was drenched with Sini Tang, the control group and the blank group with physiological saline, and the blockade group with Sini Tang plus 3-MA injection blockade, to observe LVEDV, LVESV, CO and LVEF of cardiac ultrasound in different groups at 8 h, 16 h and 24 h; and to detect the conditions of P62, LC3-? and LC3-Ⅱ of myocardial autophagy protein using WB. Results1）After intervention for 8 and 16 hours, left ventricular function of the experiment group was superior to that of the blank group, the control group and the blockade group (P<0.05), the difference had no statistical meaning in the control group and the blockade group (P>0.05); left ventricular function of the blank group was better than that of the control group and the blockade group (P<0.05); after intervention for 24 hours, the difference had no statistical meaning in left ventricular function between the experiment group, the control group and the blockade group (P>0.05). 2)After intervention for 8 and 16 hours, the expressions of autophagy protein of the experiment group were higher than these of the control group and the blockade group (P<0.05), the difference had no statistical meaning between the control group and the blockade group (P>0.05); after intervention for 24 hours, the difference had no statistical meaning in the expressions of autophagy protein between the experiment group, the control group and the blockade group (P>0.05). ConclusionSini Tang could improve sepsis rats' left ventricular function possibly through autophagy.

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备注/Memo

备注/Memo:
江四华（1991—），男，硕士学位，主治医师。研究方向：危急重症的中医药防治。贵州省科技支撑计划项目（黔科合支撑〔2021〕一般016）；贵州省卫生健康委科学技术基金项目（gzwkj 2022-018）。
更新日期/Last Update: 2023-11-15