[1]虎勤,邓婕,杨志军,等.“相恶”配伍黄芩生姜对湿热模型大鼠胃肠功能的影响[J].西部中医药,2024,37(05):47-51.[doi:10.12174/j.issn.2096-9600.2024.05.07]
　HU Qin,DENG Jie,YANG Zhijun,et al.Effects of Huangqin and Ginger on Gastrointestinal Function in Rat Models with Dampness-heat based on "Mutual Inhibition"[J].Western Journal of Traditional Chinese Medicine,2024,37(05):47-51.[doi:10.12174/j.issn.2096-9600.2024.05.07]
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“相恶”配伍黄芩生姜对湿热模型大鼠胃肠功能的影响
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
37
期数:
2024年05期
页码:
47-51
栏目:
基础研究
出版日期:
2024-05-15

文章信息/Info

Title:
Effects of Huangqin and Ginger on Gastrointestinal Function in Rat Models with Dampness-heat based on "Mutual Inhibition"
作者:
虎勤1, 邓婕2, 杨志军2, 张瑞欣2, 何建桥2, 杨秀娟2, 吴溪玮2
1.兰州现代职业学院卫生健康学院，甘肃 兰州 730300
2.甘肃中医药大学药学院，甘肃 兰州 730000
Author(s):
HU Qin1, DENG Jie2, YANG Zhijun2, ZHANG Ruixin2, HE Jianqiao2, YANG Xiujuan2, WU Xiwei2
1.Lanzhou Modern Vocational College, Lanzhou 730300, China
2.School of Pharmacy, Gansu University of Chinese Medicine, Lanzhou 730000, China
关键词:
胃肠功能; 湿热型; 黄芩; 生姜; 配伍; 大鼠
Keywords:
gastrointestinal function; dampness-heat pattern; ginger; compatibility; rat
分类号:
R285.5
DOI:
10.12174/j.issn.2096-9600.2024.05.07
文献标志码:
A
摘要:
目的比较黄芩与生姜配伍前后对湿热模型大鼠胃肠功能的影响，探究两药“相恶”内涵。 方法将50只SD大鼠随机分成正常组、模型组、黄芩组，不同配比黄芩生姜组（1∶1及2∶1），每组10只。采用复合因素法制备大鼠胃肠湿热模型，造模成功后连续7天给予相应药物灌胃，每日1次；取材后，苏木精-伊红染色法（hematoxylin-eosin staining，HE）观察各组大鼠胃、结肠组织病理学形态变化情况；计算大鼠胃内残留率；硝酸还原酶法检测大鼠血清一氧化氮（nitric oxide，NO）水平；酶联免疫吸附试验（enzyme linked immunosorbent assay，ELISA）测定各组大鼠血清肿瘤坏死因子α（tumor necrosis factor α，TNF-α）、白细胞介素10（interleukin-10，IL-10）等炎症因子表达水平；ELISA法检测胃及结肠组织神经肽P物质（substance P，SP）、血管活性肠肽（vasoactive intestinal peptide，VIP）、生长抑素（somatostatin，SS）、胃泌素（gastrin，GAS）等胃肠激素含量。 结果干预后，与模型组比较，黄芩组、黄芩生姜1∶1组大鼠胃残留率降低（P＜0.05），黄芩水煎液及黄芩生姜1∶1水煎液可下调模型大鼠血清NO、TNF-α表达水平，上调IL-10表达水平（均P＜0.05），黄芩生姜1∶1水煎液可降低模型大鼠胃及结肠组织VIP、SS含量，升高SP、GAS含量（均P＜0.05）；与黄芩水煎液及黄芩生姜1∶1水煎液比较，黄芩生姜2∶1水煎液下调血清NO、胃及结肠组织VIP、SP水平，上调结肠组织CAS水平不明显（P＞0.05）。 结论黄芩水煎液、黄芩生姜1∶1水煎液能改善湿热大鼠胃肠功能状态，主要通过调节胃肠激素、炎症因子水平发挥作用，且黄芩生姜2∶1配伍时，两药相恶减效，清热燥湿作用减弱。
Abstract:
ObjectiveTo compare the effects of Huangqin (Scutellariae radix) and ginger on the gastrointestinal function of rats modeled with dampness-heat before and after compatibility, and to investigate the connotations of "mutual inhibition" between the two drugs. MethodsFifty SD rats were randomly divided into the normal group, the model group, Huangqin group, Huangqin-ginger group with different ratios (1:1, 2:1), ten rats in each group. Rat models with gastrointestinal damp-heat were established using composite factors. The corresponding drugs were given by gavage once a day for seven consecutive days after modeling; after sampling, HE was applied to observe the histopathological and morphological changes in the stomach and colon of rats in each group under the light microscope; to calculate the residual rate in the stomach; nitrate reductase method was used to detect the levels of serum NO; ELISA was adopted to detect the expressions of TNF-α and IL-10 in the serum in different groups; ELISA was used to measure the contents of SP, VIP, SS, GAS in the stomach and colon. ResultsAfter the intervention, compared with the model group, the residual rate of the stomach decreased in Huangqin group, and Huangqin-ginger at the ratio of 1:1 (P<0.05), Huangqin decoction and Huangqin-ginger decoction at the ratio of 1:1 down-regulated the levels of NO and TNF-α, and up-regulated the levels of IL-10 in rats (all P<0.05), Huangqin-ginger decoction at the ratio of 1:1 decreased the contents of VIP and SS in the stomach and colon of rats with dampness-heat and elevated the contents of SP and GAS (all P<0.05). Compared with Huangqin-ginger decoction and Huangqin-ginger decoction at the ratio of 1:1, Huangqin-ginger decoction at the ratio of 2:1 down-regulated the levels of serum NO, the levels of VIP and SP in stomach and colon, up-regulated the levels of CAS in colon insignificantly (P>0.05). ConclusionHuangqin decoction and Huangqin-ginger decoction at the ratio of 1:1 can improve the state of gastrointestinal function in rats with dampness-heat, mainly through regulating the levels of gastrointestinal hormones and inflammatory factors. When Huangqin was combined with ginger at the ratio of 2:1, the two drugs inhibited each other, and the effect of clearing heat and drying dampness was weakened.

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备注/Memo

备注/Memo:
虎勤（1972—），女，副教授。研究方向：中药配伍及复方的临床应用研究。国家自然科学基金地区基金（82160755）；甘肃省中医药管理局科研基金（GZK-2019-20）；甘肃省教育厅高等教育教学成果培育项目（2020-120）；甘肃省教育厅科技创新项目（2022A-068）。
更新日期/Last Update: 2024-05-15