[1]杨婕,秦玲玲,吴丽丽,等.基于网络药理学探讨不忘散加味方治疗2型糖尿病轻度认知障碍的机制[J].西部中医药,2025,38(05):24-30.[doi:10.12174/j.issn.2096-9600.2025.05.07]
　YANG Jie,QIN Lingling,WU Lili,et al.The Mechanism of Treating Mild Cognitive Impairment of Type Two Diabetes Mellitus with Modified Buwang Powder Based on Network Pharmacology[J].Western Journal of Traditional Chinese Medicine,2025,38(05):24-30.[doi:10.12174/j.issn.2096-9600.2025.05.07]
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基于网络药理学探讨不忘散加味方治疗2型糖尿病轻度认知障碍的机制()

《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
38
期数:
2025年05期
页码:
24-30
栏目:
二次研究
出版日期:
2025-05-15

文章信息/Info

Title:
The Mechanism of Treating Mild Cognitive Impairment of Type Two Diabetes Mellitus with Modified Buwang Powder Based on Network Pharmacology
作者:
杨婕1, 秦玲玲2, 吴丽丽3, 胡圣涓4, 王翔云1, 胡燕1, 刘铜华3
1.北京中医药大学东方医院，北京 100078
2.北京中医药大学科技处，北京 100029
3.北京中医药大学教育部中医养生学重点实验室，北京 102488
4.北京中医药大学东直门医院，北京 100029
Author(s):
YANG Jie1, QIN Lingling2, WU Lili3, HU Shengjuan4, WANG Xiangyun1, HU Yan1, LIU Tonghua3
1.Dongfang Hospital of Beijing University of Chinese Medicine, Beijing 100078, China
2.Science and Technology Department, Beijing University of Chinese Medicine, Beijing 100029, China
3.Key Laboratory of Health Cultivation, Ministry of Education, Beijing University of Chinese Medicine, Beijing 102488, China
4.Dongzhimen Hospital of Beijing University of Chinese Medicine, Beijing 100029, China
关键词:
2型糖尿病轻度认知障碍; 不忘散加味方; 作用机制; 网络药理学; 分子对接技术
Keywords:
type 2 diabetes mellitus-induced mild cognitive impairment; modified powder; mechanism; network pharmacology; molecular docking technology
分类号:
R255.4
DOI:
10.12174/j.issn.2096-9600.2025.05.07
文献标志码:
A
摘要:
目的基于网络药理学和分子对接技术探究不忘散加味方治疗2型糖尿病（type 2 diabetes mellitus，T2DM）轻度认知障碍的作用机制。 方法利用中药系统药理学数据库与分析平台（traditional Chinese medicine systems pharmacology database and analysis platform，TCMSP）和BATMAN-TCM数据库筛选不忘散加味方的药物成分及潜在作用靶点；通过在线人类孟德尔遗传数据库（online mendelian inheritance in man，OMIM）及人类基因数据库（the human gene database，GeneCards）筛选疾病相关靶点，获取疾病与药物交集靶点，得出不忘散加味方治疗T2DM轻度认知障碍的潜在作用靶点；通过Cytoscape 3.8.2软件构建“药物-成分-作用靶点”网络；将疾病与药物交集靶点导入STRING数据库，构建蛋白-蛋白互作（proteinprotein interactions，PPI）网络，筛选核心靶点；借助核心靶基因导入基因功能注释数据库（the database for annotation visualization and integrated discovery，DAVID）对疾病与药物交集靶点做基因本体论（gene ontology，GO）和京都基因与基因组百科全书（kyoto encyclopedia of genes and genomes，KEGG）通路富集分析；通过AutoDock Vina软件对筛选出的关键成分与靶点进行分子对接，验证药物与疾病的作用关系。 结果共筛选得到不忘散加味方活性成分135个，不忘散加味方与T2DM轻度认知障碍交集靶点293个；获得槲皮素、β-谷甾醇、山柰酚、木犀草素、豆甾醇等关键活性化合物；筛选得到TP53、IL-6、AKT1、TNF、STAT3、IL-1β、EGFR、JUN、INS、BCL2等核心靶点；潜在作用靶点主要富集在脂质与动脉粥样硬化通路、糖尿病并发症AGE-RAGE、流体剪切应力与动脉粥样硬化通路等。 结论不忘散加味存在多种活性成分，可能通过多个潜在靶点、多条信号通路发挥对T2DM合并轻度认知障碍的治疗作用。
Abstract:
ObjectiveTo survey the mechanism of treating type 2 diabetes mellitus (T2DM)-induced mild cognitive impairment with modified Buwang powder based on network pharmacology and molecular docking technology. MethodsTCMSP and BATMAN-TCM databases were utilized to screen the herbal ingredients and the potential targets of actions of modified Buwang powder; disease-related targets were screened via OMIM and GeneCards, to obtain the potential targets of action for the treatment of T2DM-induced mild cognitive impairment with modified Buwang powder after gaining the intersecting targets of disease and herbs; the network of "medicine-ingredients-the targets of action" was constructed via Cytoscape 3.8.2 software; PPI network was built after introducing the intersected targets of diseases and herbs into String database, to screen out the core targets; GO and KEGG of the intersected targets of disease and herbs were carried out after importing the core target genes into DAVID; molecular docking of the selected key ingredients and targets was performed via AutoDock Vina software to verify the relationship between the action of drugs and diseases. ResultsThe study has yielded 135 kinds of active ingredients of the prescriptions, 293 intersected targets between modified Buwang powder and T2DM-induced mild cognitive impairment, involving the key active compounds including quercetin, β-sitosterol, and kaempferol, lignocerol, and stigmasterol, as well as the core targets such as TP53, IL-6, AKT1, TNF, STAT3, IL-1β, EGFR, JUN, INS and BCL2; and the potential targets of action were mainly enriched in lipids and the atherosclerotic pathway, diabetic complications AGE-RAGE, fluid shear stress and atherosclerotic pathways and others. ConclusionMany kinds of active ingredients of modified Buwang powder could treat T2DM and mild cognitive impairment possibly through many potential targets and signaling pathways.

备注/Memo

备注/Memo:
杨婕（1987—），女，硕士学位，副主任医师。研究方向：内分泌代谢疾病的中医药防治。教育部高等学校学科创新引智计划项目（B20055）。
更新日期/Last Update: 2025-05-15