[1]陈诗,李素领,刘江凯,等.小柴胡汤治疗原发性肝癌癌性发热的网络药理机制研究[J].西部中医药,2025,38(09):54-59.[doi:10.12174/j.issn.2096-9600.2025.09.11]
　CHEN Shi,LI Suling,LIU Jiangkai,et al.Network Pharmacology-based Study on Xiaochaihu Decoction in the Treatment of Primary Hepatocarcinoma Fever[J].Western Journal of Traditional Chinese Medicine,2025,38(09):54-59.[doi:10.12174/j.issn.2096-9600.2025.09.11]
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小柴胡汤治疗原发性肝癌癌性发热的网络药理机制研究()

《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
38
期数:
2025年09期
页码:
54-59
栏目:
二次研究
出版日期:
2025-09-15

文章信息/Info

Title:
Network Pharmacology-based Study on Xiaochaihu Decoction in the Treatment of Primary Hepatocarcinoma Fever
作者:
陈诗1, 李素领2, 刘江凯2, 陈欣菊2, 王振2, 赵玥1
1.河南中医药大学，河南 郑州 450000
2.河南中医药大学第一附属医院，河南 郑州 450000
Author(s):
CHEN Shi1, LI Suling2, LIU Jiangkai2, CHEN Xinju2, WANG Zhen2, ZHAO Yue1
1.Henan University of Chinese Medicine, Zhengzhou 450000, China
2.The First Affiliated Hospital of Henan University of CM, Zhengzhou 450000, China
关键词:
原发性肝癌; 癌性发热; 小柴胡汤; 网络药理学; 作用机制
Keywords:
primary hepatocarcinoma; cancerous fever; decoction; network pharmacology; mechanism
分类号:
R273
DOI:
10.12174/j.issn.2096-9600.2025.09.11
文献标志码:
A
摘要:
目的运用网络药理学与分子对接技术探讨小柴胡汤治疗原发性肝癌癌性发热（primary hepatocarcinoma fever，PHF）的作用机制。 方法利用中药系统药理学分析平台（traditional Chinese medi-cine systems pharmacology database and analysis platform，TCMSP）获取小柴胡汤含有的活性成分及相应靶点，通过与PHF疾病靶点进行交集分析，筛选小柴胡汤治疗PHF的潜在靶点及活性成分；通过UniProt、人类基因数据库（the human gene database，GeneCards）、DrugBank数据库以及Cytoscape软件分析，构建药物-有效活性成分-核心靶点-疾病网络图；借助Metascape数据库对关键靶点进行基因本体论（gene ontology，GO）和京都基因与基因组百科全书（Kyoto encyclopedia of genes and genomes，KEGG）富集分析；通过AutoDock Vina对小柴胡汤主要活性成分与关键靶点进行分子对接验证。 结果共筛选出小柴胡汤中药活性成分193个和药物疾病交集靶点144个。GO分析涉及氧化应激、细胞分化及凋亡、免疫功能及炎症反应等多个方面，KEGG富集分析主要以磷脂酰肌醇3-激酶/蛋白激酶B（phosphatidylinositol 3-kinase/protein kinase B，PI3K/AKT）、低氧诱导因子1（hypoxia-inducible factor-1，HIF-1）等信号通路为主。分子对接结果表明，小柴胡汤主要活性成分与关键靶点结合展现出较好的亲和力。 结论小柴胡汤治疗PHF具有多成分、多靶点、多通路的特点，其主要活性成分与关键靶点结合，进而调控PI3K/AKT、HIF-1、TP53等信号通路参与调节机体炎症反应、免疫功能、氧化应激、诱导细胞分化及凋亡。
Abstract:
ObjectiveTo discuss the mechanism of Xiaochaihu decoction in the treatment of primary hepatocarcinoma fever (PHF) using network pharmacology and molecular docking technology. MethodsThe active ingredients and the corresponding targets of Xiaochaihu decoction were obtained using TCMSP, to screen the potential targets and active ingredients of the treatment of PHF with the decoction after interacting with PHF-related targets; medicine-effective active ingredients-core targets-disease network diagram was constructed via UniProt, GeneCards, DrugBank database and Cytoscape software; Metascape database was applied to conduct GO and KEGG enrichment analysis of the key targets; molecular docking of the main active ingredients and key targets of Xiaochaihu decoction was validated via AutoDock Vina. ResultsA total of 193 active ingredients of Xiaochaihu decoction and 144 intersecting targets between medicine and diseases were screened. GO analysis involved many aspects including oxidative stress, cell differentiation and apoptosis, immune function and inflammatory response, KEGG enrichment analysis mainly focused on PI3K/AKT and HIF-1 signaling pathway. Molecular docking results demonstrated that the main active ingredients of Xiaochaihu decoction showed good affinity to the key targets. ConclusionThe combination of main active ingredients of Xiaochaihu decoction with key targets participates in the regulation of inflammatory response of human body, immune function, oxidative stress, inducing cellular differentiation and apoptosis via adjusting PI3K/AKT, HIF-1 and TP53 signaling pathway, which presents the characteristics of multi-ingredient, multi-target and multi-pathway in the treatment of PHF.

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备注/Memo

备注/Memo:
陈诗（1996—），女，硕士学位。研究方向：中医药防治消化系统疾病。国家自然科学基金联合基金（U1504825）；国家科技重大专项（2018ZX10303502）；河南省中医药科学研究专项课题（2021ZY1011，2022ZY1002）。
更新日期/Last Update: 2025-09-15