[1]高世龙,杨艳玲,蔡钦钦,等.基于GEO差异表达基因与网络药理学探讨黄芩经铁死亡途径调控2型糖尿病作用机制[J].西部中医药,2025,38(10):81-89.[doi:10.12174/j.issn.2096-9600.2025.10.14]
　GAO Shilong,YANG Yanling,CAI Qinqin,et al.The Mechanism of Huangqin Regulation in Type 2 Diabetes Mellitus via Ferroptosis Pathway Based on GEO Differentially Expressed Genes and Network Pharmacology[J].Western Journal of Traditional Chinese Medicine,2025,38(10):81-89.[doi:10.12174/j.issn.2096-9600.2025.10.14]
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基于GEO差异表达基因与网络药理学探讨黄芩经铁死亡途径调控2型糖尿病作用机制()

《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
38
期数:
2025年10期
页码:
81-89
栏目:
二次研究
出版日期:
2025-10-15

文章信息/Info

Title:
The Mechanism of Huangqin Regulation in Type 2 Diabetes Mellitus via Ferroptosis Pathway Based on GEO Differentially Expressed Genes and Network Pharmacology
作者:
高世龙1, 杨艳玲2, 蔡钦钦3, 金小健4, 张骥驰5, 孟馥芬6
1.青海红十字医院，青海 西宁 810000
2.高原医学研究中心，青海 西宁 810000
3.上海市第一人民医院，上海 200080
4.南方医科大学第七附属医院，广东 佛山 528243
5.郑州人民医院，河南 郑州 450000
6.新疆医科大学附属肿瘤医院/新疆医科大学第三临床医学院，新疆 乌鲁木齐 830011
Author(s):
GAO Shilong1, YANG Yanling2, CAI Qinqin3, JIN Xiaojian4, ZHANG Jichi5, MENG Fufen6
1.Qinghai Red Cross Hospital, Xi’ning 810000, China
2.Plateau Medical Research Center, Xi’ning 810000, China
3.Shanghai General Hospital, Shanghai 200080, China
4.The Seventh Affiliated Hospital of Southern Medical University, Foshan 52824
关键词:
2型糖尿病; 黄芩; 铁死亡; 网络药理学
Keywords:
type 2 diabetes mellitus; ferroptosis; network pharmacology
分类号:
R255.4
DOI:
10.12174/j.issn.2096-9600.2025.10.14
文献标志码:
A
摘要:
目的基于高通量基因表达（gene expression omnibus，GEO）差异基因结合网络药理学探讨黄芩经铁死亡途径调控2型糖尿病的作用机制。 方法基于GEO数据库筛选2型糖尿病的差异表达基因，借助数据平台筛选铁死亡相关靶点基因，通过中药系统药理学数据库与分析平台（traditional Chinese medicine systems pharmacology database and analysis platform，TCMSP）筛选黄芩的活性成分及对应靶点，取三者交集靶点基因，借助R语言和Cytoscape软件进行蛋白-蛋白互作（protein-protein interactions，PPI）网络及拓扑分析以及基因本体论（gene ontology，GO）和京都基因和基因组百科全书（kyoto encyclopedia of genes and genomes，KEGG）富集分析；采用Maestro软件进行分子对接验证。 结果GEO数据库中筛选出3个符合要求的数据集为GSE16415、GSE23343和GSE25724，包含40个样本数据，筛选得到差异表达基因3558个；数据平台中筛选到铁死亡相关靶点1965个；筛选出黄芩36个活性成分和95个靶点基因；获得9个交集靶点基因、18个核心活性成分和5个Hub基因；分子对接显示汉黄芩素、黄芩素、苏荠宁黄酮、金合欢素、5，7，4’-三羟基-8-甲氧基黄酮、黄芩新素与MMP9、MAPK14有较好的结合活性，BAX与金合欢素、汉黄芩素、黄芩素、5，7，4’-三羟基-8-甲氧基黄酮有较好的结合活性；KEGG富集得到脂质与动脉粥样硬化等49条信号通路。 结论黄芩的主要活性成分可能通过MMP9、细胞色素C等靶点，作用于脂质与动脉粥样硬化等信号通路调控2型糖尿病。
Abstract:
ObjectiveTo explore the mechanism of Huangqin (Scutellariae radix) in the regulation of type 2 diabetes mellitus (T2DM) via ferroptosis pathway based on GEO differentially expressed genes and network pharmacology. MethodsDifferentially expressed genes in T2DM were identified using GEO database, ferroptosis-related target genes were screened using a data platform, active ingredients of Huangqin and their corresponding targets were screened using the TCMSP database for the intersection of target genes between the three, KEGG enrichment analysis and GO enrichment analysis, topology analysis, and PPI network construction were performed using R language and Cytoscape software, Maestro software was used to validate molecular docking. ResultsThree datasets that meet the requirements from GEO database were GSE16415, GSE23343 and GSE25724, which contain 40 sample data and 3558 differentially expressed genes. Through data platform screening, we identified 1965 ferroptosis-related targets, subsequent analysis revealed 36 active compounds and 95 target genes associated with Huangqin. By intersecting these datasets, we obtained 9 shared target genes, 18 core bioactive compounds, and 5 hub genes for further investigation. Molecular docking analysis revealed that wogonin, baicalein, moslo-sooflavone, acacetin, 5, 7,4’-trihydroxy-8-methoxyflavone, and neobaicalein exhibit better binding affinity for MMP9 and MAPK14, and BAX has better binding affinity for acacetin, wogonin, baicalein and 5, 7,4’-trihydroxy-8-methoxyflavone; KEGG enriched 49 signaling pathways including lipid and atherosclerosis. ConclusionThe main active ingredients in Huangqin may regulate T2DM by modulating lipid metabolism and atherosclerosis-related signaling pathways through MMP9 and cytochrome C.

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备注/Memo

备注/Memo:
高世龙（1988—），男，硕士学位，主治医师。研究方向：中医药与围术期疾病研究。新疆维吾尔自治区自然科学基金（2021D01C388）；新疆医科大学附属肿瘤医院人才队伍培养计划（RC014）。
更新日期/Last Update: 2025-10-15