[1]黄舒然,王瑞平△,邹 玺,等.蜂毒素对人胃癌细胞株BGC-823凋亡及其细胞周期的影响*[J].西部中医药,2012,25(10):15-17.
　HUANG Shu-ran,WANG Rui-ping,ZOU Xi,et al. Effects of Melittin on Apoptosis and Cell Cycle of Human Gastric Cancer Cell Line BGC-823[J].Western Journal of Traditional Chinese Medicine,2012,25(10):15-17.
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蜂毒素对人胃癌细胞株BGC-823凋亡及其细胞周期的影响*()
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
25
期数:
2012年10期
页码:
15-17
栏目:
出版日期:
2012-10-15

文章信息/Info

Title:
 Effects of Melittin on Apoptosis and Cell Cycle of Human Gastric Cancer Cell Line BGC-823
文章编号:
1004-6852(2012)10-0015-03
作者:
 黄舒然1，王瑞平1，2' target="_blank" rel="external">2△' target="_blank" rel="external">2' target="_blank" rel="external">2△，邹 玺1，2' target="_blank" rel="external">2，周锦勇1，2' target="_blank" rel="external">2
 1 南京中医药大学第一临床医学院，江苏 南京 210046； 2 江苏省中医院
Author(s):
 HUANG Shu-ran1, WANG Rui-ping1,2' target="_blank" rel="external">2' target="_blank" rel="external">2' target="_blank" rel="external">2, ZOU Xi1,2' target="_blank" rel="external">2' target="_blank" rel="external">2' target="_blank" rel="external">2, ZHOU Jin-yong1,2' target="_blank" rel="external">2' target="_blank" rel="external">2' target="_blank" rel="external">2
 1 Nanjing University of Chinese Medicine, Nanjing 210046, China;
2 Jiangsu Province Hospital of Traditional Chinese Medicine
关键词:
 ; 蜂毒素; 人胃癌细胞株BGC-823; 细胞增殖; 细胞凋亡; 细胞周期
Keywords:
 ; melittin;  human gastric cancer cell line BGC-823;  cell proliferation;  apoptosis;  cell cycle
分类号:
R730.5
文献标志码:
A
摘要:
 目的：观察蜂毒素体外对人胃癌细胞株BGC-823增殖、凋亡及细胞周期的影响。方法：采用MTT法观察不同浓度的蜂毒素(32，16，8，4，2 μg/mL)体外对人胃癌细胞株BGC-823增殖的影响，并用流式细胞仪检测其诱导的细胞凋亡和周期阻滞效应。结果：①与对照组比较，蜂毒素有抑制人胃癌细胞株BGC-823增殖的作用(P ＜0.01)，随着药物浓度的增大，其对细胞的抑制率也明显增大，呈现剂量依赖性。②蜂毒素浓度从
4 μg/mL开始，即可诱导细胞产生凋亡，随着剂量的增加诱导凋亡的效果更加明显。③在细胞周期方面，与阴性对照比较，蜂毒素作用24小时后S期细胞比例上升，而G0/G1期比例明显下降，蜂毒素浓度达到16 μg/mL时G0/G1期前出现明显的亚二倍体区。结论：蜂毒素对人胃癌细胞株BGC-823的增殖具有抑制作用，且能够引起该细胞的凋亡及改变其细胞周期。
Abstract:
 Objective: To investigate effect of melittin in vitro on proliferation, apoptosis and cell cycle of human gastric cancer cell line BGC-823. Method: Effects of different concentrations of melittin (32, 16, 8, 4 or 2 μg/mL) on proliferation of human gastric cancer cell line BGC-823 were investigated with MTT assay; induced apoptosis and cycle blocking were detected with flow cytometer (FCM). Result: ① Compared with control group, melittin inhibited proliferation of human gastric cancer cell line BGC-823(P＜0.01). The inhibition effects increased with the dose. ② Melittin induced cell apoptosis when the concentration reached to 4 μg/mL, the apoptosis-inducing effect rose with the dose of melittin. ③ As cell cycle, the percentage of cells in S phase increased after melittin working for 24 hours while the ratio of the cells in G0/G1 phase decreased compared with negative control group, when the concentration of melittin reached to 16 μg/mL, the sub-diploid peak showed obviously before G0/G1 phase. Conclusion: Melittin could inhibit the proliferation of human gastric cancer cell line BGC-823, induce the apoptosis of cells and change its cell cycle.

参考文献/References:

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备注/Memo

备注/Memo:
 收稿日期：2012-04-12
*基金项目：江苏省六大高峰人才项目，江苏省中医药管理局领军人才项目(编号LJ200908)，江苏省高校优势学科资助项目。
作者简介：黄舒然(1986—)，女，硕士研究生。研究方向：肿瘤疾病的中西医结合诊治。
△通讯作者：王瑞平(1961—)，女，博士研究生导师，博士学位，教授，主任医师。研究方向：肿瘤疾病的中西医结合诊治。
更新日期/Last Update: 2012-10-29