[1]王宇,张玉兰△.HPLC法测定党参中5-羟甲基糠醛的含量[J].西部中医药,2014,27(02):31-33.
　WANG Yu,ZHANG Yulan.Determination of the Contents of 5-hydroxymethylfurfural in DangShen by HPLC[J].Western Journal of Traditional Chinese Medicine,2014,27(02):31-33.
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HPLC法测定党参中5-羟甲基糠醛的含量
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
27
期数:
2014年02期
页码:
31-33
栏目:
出版日期:
2014-02-15

文章信息/Info

Title:
Determination of the Contents of 5-hydroxymethylfurfural in DangShen by HPLC
文章编号:
1004-6852(2014)02-0031-03
作者:
王宇，张玉兰△
甘肃中医学院中西医结合系，甘肃 兰州 730000
Author(s):
WANG Yu, ZHANG Yulan△
Department of Integrative Medicine in Gansu University of Traditional Chinese Medicine, Lanzhou 730000, China
关键词:
党参; 5-羟甲基糠醛; 含量测定
Keywords:
DangShen;  5-hydroxymethylfurfural;  content determination
分类号:
R285.2
文献标志码:
A
摘要:
目的：建立党参中己糖降解产物5-羟甲基糠醛(5-HMF)的含量测定方法，对党参药材中的5-羟甲基糠醛进行含量测定。方法：采用Agilent Extend-C18(250 mm×4.6 mm，5 μm)色谱柱，以乙腈-0.3%醋酸溶液(3∶97)为流动相，检测波长284 nm，流速1.0 mL/min，柱温30 ℃。结果：5-羟甲基糠醛在浓度为 2.538～63.45 μg/mL范围内与峰面积具有良好的线性关系(r =0.999 8)，精密度和重复性的RSD分别为0.8％和 2.5%。结论：该方法操作简便，结果准确可靠，可用于党参中5-羟甲基糠醛的含量测定，为进一步评价党参的安全性提供参考。
Abstract:
Objective: To establish the determination method for 5-hydroxymethylfurfural(5-HMF) in DangShen (Radix Codonopsis Pilosulae) and determine its contents. Methods: Agilent Extend-C18(250 mm×4.6 mm, 5 μm) chromatographic column was adopted with the mobile phase of acetonitrile-0.3% acetum(3:97), detection wavelength was 284nm, flow rate was 1.0mL/min, column temperature was 30℃. Results: It showed better linear relationship in the range between 2.538 and 63.45 μg/mL and peak area (r=0.999 8), accuracy and repeatability RSD were 0.8% and 2.5% respectively. Conclusion: The method, simple and reliable, could be used to the determination of 5-HMF contained in DangShen, which could provide reference for further assessment of the safety of DangShen.

相似文献/References:

[1]马琴国,安培坤,王引权△.不同采样期党参炔苷的含量动态变化分析*[J].西部中医药,2014,27(02):23.
　MA Qinguo,AN Peikun,WANG Yinquan.Dynamic Changes of the Contents of Lobetyolin Contained in DangShen at Different Sampling Periods[J].Western Journal of Traditional Chinese Medicine,2014,27(02):23.

备注/Memo

备注/Memo:
收稿日期：2013-05-07作者简介：王宇(1977—)，男，硕士学位，讲师。研究方向：中医内科学及中医药、临床疾病的教学。△通讯作者：张玉兰(1963—)，女，主治医师。研究方向：中医内科学及中医药、临床疾病的教学。
更新日期/Last Update: 2014-02-15