[1]朱双,胡凤霞△.三七多糖对2型糖尿病大鼠高同型半胱氨酸信号通路的影响[J].西部中医药,2016,29(07):15-18.
　ZHU Shuang,HU Fengxia.The Effects of Panax Polysaccharide on Homocysteine Signaling Pathway of the Rats with Type Two Diabetes Mellitus[J].Western Journal of Traditional Chinese Medicine,2016,29(07):15-18.
点击复制

三七多糖对2型糖尿病大鼠高同型半胱氨酸信号通路的影响
分享到：

《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
29
期数:
2016年07期
页码:
15-18
栏目:
出版日期:
2016-07-15

文章信息/Info

Title:
The Effects of Panax Polysaccharide on Homocysteine Signaling Pathway of the Rats with Type Two Diabetes Mellitus
文章编号:
1004-6852(2016)07-0015-04
作者:
朱双，胡凤霞△
黄石市中心医院，湖北 黄石435000
Author(s):
ZHU Shuang, HU Fengxia△
Huangshi Municipality Central Hospital, Huangshi 435000, China
关键词:
2型糖尿病; 亚甲基四氢叶酸还原酶; 胱硫醚β-合酶; 5-甲基四氢叶酸高半胱氨酸甲基转移酶; 三七多糖
Keywords:
type two diabetes mellitus;  MTHFR;  CBS;  MTR;  panax polysaccharide
分类号:
R587
文献标志码:
A
摘要:
目的：探讨三七多糖对2型糖尿病大鼠肝脏高同型半胱氨酸代谢信号通路3个关键分子亚甲基四氢叶酸还原酶(MTHFR)、胱硫醚β-合酶(CBS)、5-甲基四氢叶酸高半胱氨酸甲基转移酶(MTR)表达的影响。方法：将60只雄性SD大鼠随机分为空白组、模型组以及三七多糖治疗组，每组20只，模型组以及三七多糖治疗组给予高脂高糖饮食6个月建立2型糖尿病模型，模型建立成功后三七多糖治疗组给予三七多糖57 mg/(kg·d)灌胃治疗，模型组给予同体积生理盐水灌胃，分别在治疗4周和8周后处死大鼠各半，收集血清和肝脏进行相关指标的检测。结果：治疗4周后大鼠血糖以及胰岛素抵抗指数三七多糖治疗组较模型组降低(P＜0.05)，8周后进一步降低，胰岛素水平治疗4周后三七多糖治疗组较模型组降低(P＜0.05)，8周后较4周无进一步改善；三七多糖治疗后，大鼠叶酸较模型组有一定升高，但差异无统计学意义(P＞0.05)，维生素B12在治疗4周后较模型组明显升高(P＜0.05)，治疗8周后进一步升高；同型半胱氨酸在治疗4周后明显降低(P＜0.05)，但在8周后无进一步改善；治疗4周后，与模型对照组比较，三七多糖治疗组MTHFR、MTR以及CBSmRNA及蛋白表达升高(P＜0.05)，治疗8周后进一步升高，但未达到空白组水平。结论：三七多糖可显著降低模型大鼠血清中同型半胱氨酸含量，其可能是通过调控高同型半胱氨酸信号通路相关蛋白MTHFR、CBS、MTR的表达来实现。
Abstract:
Objective: To explore the influence of panax polysaccharide on the expressions of three key molecules MTHFR, CBS, MTR of homocysteine signaling pathway of the rats with type two diabetes mellitus (T2DM). Methods: Sixty rats were randomized into the blank group, the model group and the treatment group of panax polysaccharide, 20 rats each group, the model group and the treatment group of panax polysaccharide were given high fat and high sugar diet for six months to establish T2DM rat models, after successfully establishing the models, the treatment group of panax polysaccharide accepted intragastric administration of panax polysaccharide in the concentrations of 57mg/(kg·d), the model group received physiological saline in the same volume, the rats were sacrificed after treating in four weeks and eight weeks respectively, the related indexes were detected after collecting the serum and liver. Results: Blood glucose and insulin resistance index of the treatment group of panax polysaccharide decreased obviously compared with the model group after treating in four weeks (P＜0.05), these indexes lowered after treating in eight weeks, insulin level decreased after treating in four weeks when the treatment group of panax polysaccharide was compared with the model group notably (P＜0.05), there was no improvements after treating in eight weeks compared with these in four weeks; after treating with panax polysaccharide, the levels of folic acid of the rats rose compared with the model group, but there was no statistical meaning (P＞0.05), the levels of vitamin B12 improved compared with the model group after treating in four weeks (P＜0.05), they improved further after treating in eight weeks; the level of hemocysteine decreased obviously after treating in four weeks (P＜0.05), while there was no improvements after treating in eight weeks; compared with the model group after treating in four weeks, mRNA and protein expressions of MTHFR, MTR and CBS rose evidently in the treatment group of panax polysaccharide (P＜0.05), these expressions improved after treating in eight weeks, but they didn’t reach the level of the blank group. Conclusion: Panax polysaccharide could notably decrease the contents of homocysteine in the rat model apparently, which might be realized by regulating the expressions of MTHFR, MTR and CBS in homocysteine signaling pathway.

相似文献/References:

[1]祁瑞芳.同型半胱氨酸、超敏 C- 反应蛋白在 2 型糖尿病合并脑梗死预测中的作用[J].西部中医药,2014,27(08):152.
[2]邱勇玉,李应东,赵信科,等.高血压病中医证型与 2 型糖尿病及其相关危险因素分析[J].西部中医药,2014,27(09):75.
[3]李东峰,指导：王志刚.王志刚主任医师治疗2型糖尿病“三级”防治思想辨治心得[J].西部中医药,2015,28(01):29.
[4]周雨聪,指导：刘召.红灵五黄汤治疗2型糖尿病90例[J].西部中医药,2015,28(04):99.
[5]杨国军.黄芪注射液对2型糖尿病患者血管功能及微循环状态的影响[J].西部中医药,2015,28(05):109.
[6]康学东,张瀚文.病证结合探讨2型糖尿病胰岛素抵抗[J].西部中医药,2015,28(11):112.
[7]张喜芬,赵保礼,杨立波,等.通络明目胶囊治疗糖尿病视网膜病变的随机双盲对照研究[J].西部中医药,2013,26(11):88.
　ZHANG Xifen,ZHAO Baoli,YANG Libo,et al.A Randomized, Double-blind and Placebo-controlled Study of TongLuo MingMu Capsule in Treating Diabetic Retinopathy[J].Western Journal of Traditional Chinese Medicine,2013,26(07):88.
[8]戴梅.全面干预护理对2型糖尿病患者血糖和糖化血红蛋白水平的影响[J].西部中医药,2013,26(07):109.
　DAI Mei.Intervention of Comprehensive Nursing on Blood Glucose and Glycosylated Hemoglobin of Patients with Diabetes Mellitus Type Two[J].Western Journal of Traditional Chinese Medicine,2013,26(07):109.
[9]张家林,裴瑞霞.银花痛风颗粒治疗2型糖尿病合并高尿酸血症疗效观察[J].西部中医药,2014,27(01):106.
　ZHANG Jialin,PEI Ruixia.Clinical Observation on YinHua TongFeng Granules in Treating Type 2 Diabetes Mellitus Complicated with Hyperuricemia[J].Western Journal of Traditional Chinese Medicine,2014,27(07):106.
[10]祁瑞芳.同型半胱氨酸、超敏C-反应蛋白在2型糖尿病合并脑梗死预测中的作用[J].西部中医药,2014,27(04):136.
　QI Ruifang.Diagnostic Value of Homocysteine and High Sensitivity C-Reactive Protein in Predicting Type 2 Diabetes Mellitus Complicated with Cerebral Infarction[J].Western Journal of Traditional Chinese Medicine,2014,27(07):136.

备注/Memo

备注/Memo:
收稿日期：2015-10-15 *基金项目：湖北省科技厅资助项目(编号2012FFA016)。 作者简介：朱双(1776—)，女，主管护师。研究方向：糖尿病的护理。 △通讯作者：胡凤霞(1972—)，女，副主任护师。研究方向：慢性肾脏病及血透、糖尿病的护理。
更新日期/Last Update: 2016-07-15