[1]王和宇,李春娜,秦玲玲,等.油橄榄叶中活性成分的提取工艺考察[J].西部中医药,2017,30(03):39-42.
　WANG Heyu,LI Chunna,et al.Extraction Technology for Active Components in Olive Leaves[J].Western Journal of Traditional Chinese Medicine,2017,30(03):39-42.
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油橄榄叶中活性成分的提取工艺考察()
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
30
期数:
2017年03期
页码:
39-42
栏目:
出版日期:
2017-03-15

文章信息/Info

Title:
Extraction Technology for Active Components in Olive Leaves
文章编号:
1004-6852(2017)03-0039-04
作者:
王和宇1，2，李春娜1，3，秦玲玲1，张晶2，徐暾海1，3△，刘铜华1
1 北京中医药大学中医养生学教育部重点实验室，北京 100029；2 吉林农业大学中药材学院； 3 北京中医药大学中药学院
Author(s):
WANG Heyu 1, 2, LI Chunna 1, 3, QIN Lingling1, ZHANG Jing 2, XU Dunhai1, 3△, LIU Tonghua1
1 Key Laboratory of TCM Health Education Ministry, Beijing University of Traditional Chinese Medicine, Beijing 100029, China; 2 Chinese Medicinal College, Jilin Agricultural University; 3 School of Traditional Chinese Materia Medica, Beijing University of Traditional Chinese Medicine
关键词:
油橄榄叶; HPLC; 木犀草苷; 橄榄苦苷; 超声波提取法; 正交试验; 提取工艺考察
Keywords:
olive leaves;  HPLC;  galuteolin;  oleuropein;  ultrasonic extracting method;  orthogonal test;  study of the extraction technology
分类号:
R284
文献标志码:
A
摘要:
目的：考察油橄榄(Olea europaea Linn)叶中活性成分的最佳提取工艺。方法：采用HPLC法测定油橄榄叶中木犀草苷、橄榄苦苷，并使用超声波提取法进行提取，考察料液比，溶剂分数，提取时间，超声功率对含量的影响。在单因素基础上，结合正交试验考察溶剂分数、料液比、提取时间对橄榄苦苷含量的影响，确定最佳提取工艺。结果：油橄榄叶中活性成分的最佳提取工艺为80%甲醇溶液30 mL，超声400W提取30分钟，提取次数1次。结论：采用该方法提取油橄榄叶中活性成分简单易行。
Abstract:
Objective: To study the best extraction technology for active components in olive leaves. Methods: Galuteolin, oleuropein in olive leaves were determined by HPLC method, and extracted by ultrasonic method. Effects of the solid-liquid ratio, solvent fraction, extraction time and ultrasonic power on contents were inspected. Based on single factor tests and combined with orthogonal test, effects of the solvent fraction, solid-liquid ratio and extraction time on the content of oleuropein were inspected to determine the best extraction technology. Results: The best extraction technology for active components in olive leaves was extracting for 30min with 30mL 80% methanol solution under the condition of 400W ultrasound, and the extraction time was once. Conclusion: This method is simple and practicable for extracting the active components in olive leaves.

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备注/Memo

备注/Memo:
收稿日期：2016-06-16 *基金项目：国家国际科技合作专项项目(编号2012DFG31550)；北京中医药大学创新团队项目(编号2011-CXTD-19)。 作者简介：王和宇(1989—)，男，在读硕士研究生。研究方向：天然产物化学。 △通讯作者：徐暾海(1969—)，男，博士研究生导师，教授。研究方向：糖尿病及其并发症的中药干预及新药研究。
更新日期/Last Update: 2017-03-15