[1]崔小平,张骐,王金红△.雏菊叶龙胆酮对STZ诱导的糖尿病大鼠胰腺的保护作用[J].西部中医药,2017,30(11):40-43.
　CUI Xiaoping,ZHANG Qi,WANG Jinhong.The Safety Action of Bellidifolin on the Pancreas of STZ-induced DM Rats[J].Western Journal of Traditional Chinese Medicine,2017,30(11):40-43.
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雏菊叶龙胆酮对STZ诱导的糖尿病大鼠胰腺的保护作用()
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
30
期数:
2017年11期
页码:
40-43
栏目:
出版日期:
2017-11-15

文章信息/Info

Title:
The Safety Action of Bellidifolin on the Pancreas of STZ-induced DM Rats
文章编号:
1004-6852(2017)11-0040-04
作者:
崔小平，张骐，王金红△
潍坊医学院药理教研室，山东 潍坊 261000
Author(s):
CUI Xiaoping, ZHANG Qi, WANG Jinhong△
Pharmacology Teaching and Research Section, Weifang Medical University, Weifang 261000, China
关键词:
糖尿病大鼠; 瘤毛獐牙菜; 雏菊叶龙胆酮
Keywords:
diabetic rats;  LiuMao ZhangYaCai;  bellidifolin
分类号:
R966
摘要:
目的：探讨瘤毛獐牙菜中分离所得的雏菊叶龙胆酮对糖尿病大鼠胰腺的保护作用机制。方法：将雄性Wistar大鼠随机分为正常组、模型组、雏菊叶龙胆酮(50 mg/kg)组，每组8只。模型组和雏菊叶龙胆酮组建立链脲佐菌素(STZ)诱导的糖尿病模型，雏菊叶龙胆酮组用雏菊叶龙胆酮进行干预，检测各组大鼠血清抗氧化因子超氧化物歧化酶(SOD)和过氧化氢酶(CAT)的活性以及氧化代谢产物丙二醛(MDA)浓度，同时对胰腺组织HE染色并应用免疫印迹研究损伤相关因子NF-κBp65 以及IκBα蛋白表达量的差异，分析雏菊叶龙胆酮对胰腺的保护作用。结果：雏菊叶龙胆酮能提高糖尿病大鼠血清CAT、SOD的活性(P＜0.01)，能降低MDA的浓度(P＜0.01)；胰腺HE染色显示雏菊叶龙胆酮能够减少糖尿病大鼠胰岛的萎缩，免疫印迹显示雏菊叶龙胆酮能增加胰腺组织内IκBα的表达，降低NF-κBp65蛋白表达。结论：雏菊叶龙胆酮可能通过胰腺NF-κB信号通路来减轻氧化应激水平，从而减轻STZ诱导的胰岛损伤，对胰岛起到保护作用。
Abstract:
Objective: To study the protective mechanism of bellidifolin separated from LiuMao ZhangYaCai (Swertia pseudochinensis Hara) for the pancreas of diabetic rats. Methods: The male Wistar rats were randomized into the normal group, the model group and bellidifolin group (50 mg/kg), eight rats each group. The rats in the model group and bellidifolin group were established by STZ into diabetic models, bellidifolin group were intervened by bellidifolin. The protective effects of bellidifolin to the pancreas were analyzed by detecting the concentrations of oxidation metabolite malonaldehyde (MDA), the activity of serum antioxidant factor SOD and catalase (CAT) of the rats in different groups, studying the difference between the contents of damage related factors NF-κBp65 and IκBα protein expressions after HE staining of the pancreatic island by western blotting method. Results: Bellidifolin could improve the activity of SOD and CAT (P＜0.01), decrease the concentrations of MDA (P＜0.01); HE staining of the pancreatic island showed that bellidifolin could relieve the atrophy of pancreatic island of diabetic rats, Western blotting demonstrated that bellidifolin could increase the expressions of IκBα in pancreatic tissue and decrease the expressions of NF-κBp65 protein. Conclusion: Bellidifolin could alleviate STZ-induced damage to pancreatic island and protect pancreatic island, which might ease oxidative stress level through pancreatic island NF-κB signal channel.

备注/Memo

备注/Memo:
收稿日期：2016-12-14 作者简介：崔小平(1990—)，女，在读硕士研究生。研究方向：药理学。 △通讯作者：王金红(1972—)，女，博士学位，副教授。研究方向：药理学。
更新日期/Last Update: 2017-11-15