[1]宋元春,赛依帕△,高霞,等.黄芪总苷对阿霉素诱导损伤足细胞的保护作用[J].西部中医药,2018,31(03):19-22.
　SONG Yuanchun,SAI Yipa,GAO Xia,et al.Protecting Effects of Total Astragalus Glycosides on Adriamycin-induced Podocytes Injury[J].Western Journal of Traditional Chinese Medicine,2018,31(03):19-22.
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黄芪总苷对阿霉素诱导损伤足细胞的保护作用()
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
31
期数:
2018年03期
页码:
19-22
栏目:
出版日期:
2018-03-15

文章信息/Info

Title:
Protecting Effects of Total Astragalus Glycosides on Adriamycin-induced Podocytes Injury
文章编号:
1004-6852(2018)03-0019-04
作者:
宋元春，赛依帕△，高霞，罗璇
甘肃省人民医院儿科，甘肃 兰州 730000
Author(s):
SONG Yuanchun, SAI Yipa△, GAO Xia, LUO Xuan
Pediatric Department, Gansu Provincial Hospital, Lanzhou 730000, China
关键词:
足细胞; 凋亡相关蛋白; 基质金属蛋白酶; 黄芪总苷; 阿霉素
Keywords:
podocyte;  apoptosis related protein;  matrix metalloproteinase;  total astragalus glycosides;  adriamycin
分类号:
R285.5
摘要:
目的：探讨黄芪总苷保护阿霉素(ADR)诱导损伤足细胞的分子作用机制。方法：采用0.5 μmol/L的ADR处理小鼠足细胞系(MPC5)条件永生型小鼠足细胞系24小时，建立足细胞损伤模型；以50、100、200 μg/mL3个浓度的黄芪总苷分别处理ADR损伤足细胞24小时后，采用酶联免疫吸附法(ELISA)检测MMP2及MMP9表达水平；采用蛋白质印迹法(Western blot)检测各组凋亡相关蛋白表达水平及MT1、Nephrin蛋白质表达水平。结果：ELISA结果显示，基质金属蛋白酶(MMPs)表达水平受ADR抑制，各浓度黄芪总苷可改善该异常表达；蛋白质印迹实验结果显示，ADR可上调促凋亡相关蛋白表达水平，下调MT1、Nephrin及凋亡抑制相关蛋白的表达水平，各浓度黄芪总苷可显著改善各蛋白质的异常表达。结论：黄芪总苷对ADR损伤的MPC5足细胞具有保护作用，这可能与黄芪总苷可以下调ADR损伤足细胞Bax、P53蛋白表达水平并上调MMP-2、MMP-9、MT1及Nephrin等蛋白表达水平有关。
Abstract:
Objective: To discuss molecular mechanism of total astragalus glycosides (AST) protecting adriamycin (ADR)-induced podocytes injury. Methods: Podocyte injury model was established by dealing MPC5 conditional immortality type podocyte series with ADR, 0.5 μmol/L, for 24 hours; the levels of MMP2 and MMP9 were detected by ELISA method after dealing ADR-induced podocyte with AST, 50, 100 and 200 μg/mL for 24 hours respectively; the expressions of MT1, Nephrin protein and apoptosis related protein in different groups were measured by Western blot method. Results: ELISA results showed that the expressions of matrix metalloproteinase (MMPs) were inhibited by ADR, AST in different concentrations could improve the abnormal expressions; western blot methods indicated that ADR could up regulate the expressions of apoptosis-related protein, down regulate the expressions of MT1, Nephrin and apoptosis related protein, AST in different concentrations could improve the abnormal expressions of the protein. Conclusion: AST could protect ADR-induced MPC5 podocyte, and it might be related to that AST could down regulate the expressions of Bax and P53 protein, up regulate the expressions of MMP-2, MMP-9, MT1 and Nephrin protein of ADR-induced podocyte injury.

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备注/Memo

备注/Memo:
收稿日期：2017-06-15 *基金项目：甘肃省中医药管理局项目(编号G2K-2014-59)。 作者简介：宋元春(1975—)，女，主治医师。研究方向：小儿肾脏疾病的诊治。 △通讯作者：赛依帕(1973—)，女，副主任医师。研究方向：小儿肾脏疾病发病机制的研究。
更新日期/Last Update: 2018-03-15