[1]甘雨,马进△,袁媛,等.线栓法制备SD大鼠局灶性脑缺血再灌注模型的实践与评价[J].西部中医药,2018,31(07):12-14.
　GAN Yu,MA Jin,YUAN Yuan,et al.Practice and Evaluation of Focal Cerebral Ischemia Reperfusion Rat Model Prepared by Middle Cerebral Artery Occlusion[J].Western Journal of Traditional Chinese Medicine,2018,31(07):12-14.
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线栓法制备SD大鼠局灶性脑缺血再灌注模型的实践与评价()
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
31
期数:
2018年07期
页码:
12-14
栏目:
出版日期:
2018-07-15

文章信息/Info

Title:
Practice and Evaluation of Focal Cerebral Ischemia Reperfusion Rat Model Prepared by Middle Cerebral Artery Occlusion
文章编号:
1004-6852(2018)07-0012-03
作者:
甘雨，马进△，袁媛，乔敏，包玉龙，王菲
辽宁省中医药研究院，辽宁 沈阳 110034
Author(s):
GAN Yu, MA Jin△, YUAN Yuan, QIAO Min, BAO Yulong, WANG Fei
Liaoning Academy of Chinese Medicine, Shenyang 110034, China
关键词:
脑缺血再灌注; 局灶性; 线栓法; 动物模型; SD大鼠
Keywords:
cerebral ischemia reperfusion;  focal;  middle cerebral artery occlusion;  animal model;  SD rats
分类号:
R965.1
文献标志码:
A
摘要:
目的：建立一种稳定、可靠的大鼠局灶性脑缺血再灌注模型制备方法，并评价其效果。方法：用线栓阻塞SD大鼠(260～280 g)右侧大脑中动脉，2小时后再灌注，术后2小时及第3、7、14天进行神经功能评分，并通过脑组织2，3,5-氯化三苯基四氮唑(TTC)染色对模型进行评价。结果：模型组大鼠死亡率为29.6%，术后2小时，3、7、14天神经行为学评分分别为(1.94±0.80)分、(1.61±0.78)分、(1.50±0.67)分及(1.33±0.52)分，均显著高于假手术组(P＜0.01)。经TTC染色发现病灶范围较恒定，术后3、7、14天脑梗死面积占整个脑面积百分比分别为(32.61±0.76)%，(29.50±4.03)%和(13.32±3.24)%，与假手术组同期比较均有显著性差异(P＜0.01)。结论：采用西浓线栓制备过程中注重细节完善，便可得到稳定的SD大鼠局灶性脑缺血再灌注模型。
Abstract:
Objective: To introduce a stable and reliable preparation method for focal cerebral ischemia reperfusion rat model, and assess the effects. Methods: Right middle cerebral artery of SD rat was occluded, and reperfused in two hours, neurological function score was performed in two hours, three days, seven days and 14 days after the operation, and the model was assessed by TTC staining in cerebral tissue. Results: Mortality rate of the rats in the model group was 29.6%, neurological behavior scores of them in two hours after the operation, three days, seven days and 14 days were(1.94±0.80) points, (1.61±0.78) points, (1.50±0.67) points and (1.33±0.52) points, remarkably higher than these of sham operation group (P＜0.01). After TTC staining, the range of foci was constant, the percentages of cerebral infarction area occupying the entire brain area were (32.61±0.76)%, (29.50±4.03)% and (13.32±3.24)%, there was the significance difference when they were compared with these of sham operation group (P＜0.01). Conclusion: Stable rat model with focal cerebral ischemia reperfusion could be obtained by applying intraluminal thread to SD rats and paying attention to perfect details in the preparation courses.

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[1]严亚锋,白海侠.消栓通络胶囊对急性脑缺血再灌注大鼠脑组织TNF-α和IL-1β水平的影响[J].西部中医药,2013,26(09):23.
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备注/Memo

备注/Memo:
收稿日期：2016-11-27 *基金项目：辽宁省教育厅资助项目(编号L2013362)。 作者简介：甘雨(1981—)，男，硕士学位，副研究员。研究方向：中药复方药理学研究。 △通讯作者：马进(1976—)，女，博士学位，副主任医师。研究方向：脑病的中医研究。
更新日期/Last Update: 2018-07-15