[1]胡玥,邹玺,王瑞平,等.益气健脾解毒方对胃癌细胞的抑制作用及其机制研究[J].西部中医药,2019,32(01):14-18.
　HU Yue,ZOU Xi,WANG Ruiping,et al.Study on the Inhibitory Effects of Replenishing-Qi Invigorating-spleen Detoxifying Prescription on Gastric Cancer Cells and Its Mechanism[J].Western Journal of Traditional Chinese Medicine,2019,32(01):14-18.
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益气健脾解毒方对胃癌细胞的抑制作用及其机制研究
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
32
期数:
2019年01期
页码:
14-18
栏目:
出版日期:
2019-01-15

文章信息/Info

Title:
Study on the Inhibitory Effects of Replenishing-Qi Invigorating-spleen Detoxifying Prescription on Gastric Cancer Cells and Its Mechanism
文章编号:
1004-6852(2019)01-0014-05
作者:
胡玥，邹玺，王瑞平，张微，潘宇△
江苏省中医院，江苏 南京 210029
Author(s):
HU Yue, ZOU Xi, WANG Ruiping, ZHANG Wei, PAN Yu△
Jiangsu Province Hospital of TCM, Nanjing 210029, China
关键词:
胃癌; Bax; Bcl-2; Caspase-3; 益气健脾解毒方
Keywords:
gastric cancer;  Bax;  Bcl-2;  Caspase-3;  replenishing-Qi invigorating-spleen detoxifying prescription
分类号:
R329.2
文献标志码:
A
摘要:
目的：探讨益气健脾解毒方对胃癌细胞的抑制作用及其机制。方法：采用四甲基偶氮唑蓝(MTT)法观察不同浓度的益气健脾解毒方溶液体外作用于胃癌细胞株不同时间后对其增殖的抑制作用；运用流式碘化丙啶(Annexin-V/PI)双染色法研究不同浓度益气健脾解毒方对人胃癌细胞株凋亡的诱导作用；利用流式细胞仪检测益气健脾解毒方对人胃癌细胞株的周期阻滞效应；采用免疫印迹(Western blot)法观察B淋巴细胞瘤2(B-cell lymphoma，Bcl-2)、Bcl-2相关X蛋白(Bcl-2 associated X protein，Bax)、半胱氨酸天冬氨酸蛋白酶3(Cysteinyl aspartate-specific proproteases-3,Caspase-3)蛋白的变化情况。结果：益气化瘀解毒方对人胃癌细胞株MGC-803细胞有较好的抑制增殖作用(P＜0.05)，并呈一定的浓度依赖性；益气健脾解毒方在作用于人胃癌细胞24小时后，细胞早期凋亡率随着给药浓度的增加呈上升趋势，呈浓度依赖关系；表明益气健脾解毒方可阻滞MGC-803细胞于S期，并呈浓度依赖性；Bcl-2、Caspase-3在4、2、1 mg/mL组的蛋白表达下调；而Bax、Cleaved-caspase-3在4、2 、1 mg/mL组表达上调。结论：益气健脾解毒方能抑制人胃癌细胞株的增殖并诱导其凋亡，杀伤肿瘤细胞，可能是通过启动caspase的级联反应及下调Bcl-2，上调Bax来实现。
Abstract:
Objective: To discuss the inhibitory effects of replenishing-Qi invigorating-spleen detoxifying prescription on gastric cancer cells and its mechanism. Methods: The inhibitory effects of different concentrations of replenishing-Qi invigorating-spleen detoxifying prescription on the proliferation of gastric cancer cell line after the prescription acting on them in vitro for different times were observed by MTT method; the inducing effects of different concentrations of replenishing-Qi invigorating-spleen detoxifying prescription on the apoptosis of human gastric cancer cell lines were studied by Annexin-V/PI double-staining method; cycle arresting effects of the prescription on human gastric cancer cell line were detected by flow cytometry; the changes of Bax, Bcl-2and Caspase-3 were observed by Western blot method. Results:The prescription showed better inhibitory and proliferative effects to MGC-803 cells of human gastric cancer cell line (P＜0.05), and it was in a dose-dependent manner; after the prescription acting on human gastric cancer cells for 24 hours, early apoptotic rate of the cells presented the rising trend as the medication concentrations increased, and it was in a dose-dependent manner; it demonstrated that the prescription could arrest MGC-803 cells at S stage, which was in a dose-dependent manner; the expressions of Bcl-2 and Caspase-3 in the groups of 4, 2 and 1mg/mL decreased; while the expressions of Bax and Cleaved-caspase-3 in the groups of 4, 2 and 1mg/mL increased. Conclusion: Replenishing-Qi invigorating-spleen detoxifying prescription could inhibit the proliferation of human gastric cancer cell line and induce its apoptosis, kill tumor cells, which might be realized by triggering cascade reaction of caspase, lowering Bcl-2 and increasing Bax.

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备注/Memo

备注/Memo:
收稿日期：2018-07-27 *基金项目：国家中医临床研究基地业务建设科研专项立项 计划(编号JDZX2012087，JDZX2015089)；江苏省自然科学基金青年项目(编号BK20181097)。 作者简介：胡玥(1983—)，女，硕士学位，主治医师。研究方向：肿瘤的中西医结合治疗。 △通讯作者：潘宇(1978—)，男，硕士学位，副主任医师。研究方向：肿瘤的中医防治。
更新日期/Last Update: 2019-01-15