[1]张文进,孙志华,李永吉,等.丹参注射液对膝骨性关节炎关节液及炎症血清细胞因子水平的影响[J].西部中医药,2019,32(02):21-24.
　ZHANG Wenjin,SUN Zhihua,LI Yongji,et al.Influence of DanShen Injection on the Level of Serum Cytokines and Joint Fluid of Knee Osteoarthritis[J].Western Journal of Traditional Chinese Medicine,2019,32(02):21-24.
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丹参注射液对膝骨性关节炎关节液及炎症血清细胞因子水平的影响
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
32
期数:
2019年02期
页码:
21-24
栏目:
出版日期:
2019-02-15

文章信息/Info

Title:
Influence of DanShen Injection on the Level of Serum Cytokines and Joint Fluid of Knee Osteoarthritis
文章编号:
1004-6852(2019)02-0021-04
作者:
张文进1，孙志华2，李永吉2，李秋实2，段洪超2△
1 解放军第二一一医院骨二科，黑龙江 哈尔滨 150086； 2 黑龙江中医药大学附属二院
Author(s):
ZHANG Wenjin1, SUN Zhihua2, LI Yongji2, LI Qiushi2, DUAN Hongchao2△
1 The Second Orthopedic Department, 211 Hospital of PLA, Harbin 150086, China; 2 The Second Affiliated Hospital of Heilongjiang University of Chinese Medicine
关键词:
丹参注射液; 关节炎; 膝关节; 骨性; 细胞因子; 动物实验
Keywords:
DanShen injection;  osteoarthritis;  knee;  cytokine;  animal experiment
分类号:
R684.3
摘要:
目的：观察丹参注射液对大鼠膝骨性关节炎关节液及血清细胞因子水平的影响。方法：SD大鼠80只，随机分为空白组、模型组、对照组、观察组，每组20只。每周观察大鼠膝骨关节变化，并进行肉眼及镜下的膝关节评分，采用ELISA酶联免疫检测血清及关节液中炎症细胞因子(IL-1β、IL-6及TNF-α)水平。结果：1)大鼠膝关节围度测量数据，治疗第1～5周时模型组、观察组、对照组与空白组比较，差异有统计学意义(P＜0.05)；治疗第1～2周时观察组、对照组与模型组比较，差异无统计学意义(P＞0.05)；治疗第3～5周时，观察组、对照组与模型组比较，差异有统计学意义(P＜0.05)。2)鼠膝关节肉眼评分，治疗第1～5周时模型组、观察组、对照组与空白组比较，差异有统计学意义(P＜0.05)；治疗第1周时观察组、对照组与模型组比较，差异无统计学意义(P＞0.05)；观察第2～4周时，观察组、对照组与模型组比较，差异有统计学意义(P＜0.05)；对照组、观察组治疗第2～5周时与本组治疗前一时间点比较，差异均有统计学意义(P＜0.05)。3)鼠膝关节镜下评分，治疗第1～5周时模型组、观察组、对照组与空白组比较，差异有统计学意义(P＜0.05)；治疗第1周时观察组、对照组与模型组比较，差异无统计学意义(P＞0.05)；治疗第2～4周时，观察组、对照组与模型组比较，差异有统计学意义(P＜0.05)；对照组、观察组治疗第2～5周时与本组治疗前一时间点比较，差异均有统计学意义(P＜0.05)。4)模型组、观察组、对照组治疗前后鼠膝关节、血清炎症细胞因子水平始终高于空白组(P＜0.05)，并且观察组和对照组高出程度不及模型组(P＜0.05)。结论：丹参注射液能够降低大鼠血清及膝关节液中炎症细胞因子的水平起到治疗膝关节骨性关节炎的作用。
Abstract:
Objective：To observe the effects of DanShen injection on the level of serum cytokines and joint fluid of the rats with knee osteoarthritis (KOA). Methods: Eighty SD rats were randomized into the blank group, the model group, the control group and the obseroation group, 20 rats each group. The levels of cytokines (IL-1β, IL-6 and TNF-α) in joint fluid and the serum were detected using ELISA method, knee joint was scored under the naked eye and the arthroscope, the changes of knee joints were observed each week. Results: 1)Knee joint circumferences: the difference had statistical meaning when the model group, the observation group and the control group were compared with the blank group from the first to fifth week of treating(P＜0.05); the difference had no statistical meaning when the observation group and the control group were compared with the model group from the first to second week of treating (P＞0.05); the difference had statistical meaning when the observation group and the control group were compared with the model group from the third to fifth week of treating(P＜0.05). 2)The scores of rat knee joint under the naked eye, the difference had statsitcal meaning when the model group, the observation group and the control group were compared with the blank group from the first to fifth week of treating(P＜0.05); the difference had no statistical meaning when the observation group and the control group were compared with the model goup at the first week of treating (P＞0.05); from the second to forth week of treating, the obseroation group and the control group were compared with the model group, the difference had statistical meaning(P＜0.05); the difference showed statistical meaning when the control group and the observation group from the second to fifth week of treating were compared with themselves at the time before treating(P＜0.05). 3)The scores of rat knee under the arthroscope, the difference had statistical meaning when the model group, the observation group and the control group were compared with the blank group from the first to fifth week of treating(P＜0.05); the difference had no statistical meaning when the observation group and the control group were compared with the model group at the first week of treating (P＞0.05); the difference had statistical meaning when the observation group and the control group were compared with the model group from the second to the forth week of treating (P＜0.05); the difference showed statistical meaning when the control group and the observation group were compared with themselves at the same time before treating(P＜0.05). 4)The model group, the observation group and the control group were higher than the blank group in the levels of knee joint and serum cytokines before and after treating (P＜0.05), the rise of the observation group and the control group were less than that of the model group (P＜0.05). Conclusion: DanShen injection could treat KOA through lowering the levels of cytokines in the serum and joint fluid of the rats.

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备注/Memo

备注/Memo:
收稿日期：2018-07-10 *基金项目：哈尔滨市科技局青年后备人才(编号2016RQQXJ225)。 作者简介：张文进(1975—)，男，博士学位，副主任医师。研究方向：中西医结合治疗骨关节病。 △通讯作者：段洪超(1978—)，男，主治医师。研究方向：中西医结合治疗骨关节病。
更新日期/Last Update: 2019-02-15