[1]曹诗丹,徐伟△,孙维峰,等.基于caspase 1/IL-1β信号转导通路探讨清热除痹方治疗痛风性关节炎的作用机制[J].西部中医药,2019,32(08):79-81.
　CAO Shidan,XU Wei,SUN Weifeng,et al.Discussion on the Mechanism of Heat-clearing Rheumatism-eliminating Prescription in the Treatment for Gouty Arthritis Based on Caspase1/IL-1β Signal Pathway[J].Western Journal of Traditional Chinese Medicine,2019,32(08):79-81.
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基于caspase 1/IL-1β信号转导通路探讨清热除痹方治疗痛风性关节炎的作用机制
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
32
期数:
2019年08期
页码:
79-81
栏目:
出版日期:
2019-08-15

文章信息/Info

Title:
Discussion on the Mechanism of Heat-clearing Rheumatism-eliminating Prescription in the Treatment for Gouty Arthritis Based on Caspase1/IL-1β Signal Pathway
文章编号:
1004-6852(2019)08-0079-03
作者:
曹诗丹1，2，徐伟1△，孙维峰1，李静1
1 中国人民解放军南部战区总医院，广东 广州 510010； 2 广州中医药大学
Author(s):
CAO Shidan1,2, XU Wei1△, SUN Weifeng1, LI Jing1
1 General Hospital of Southern Theater Command, PLA, Guangzhou 510010, China; 2 Guangzhou University of Chinese Medicine
关键词:
痛风; 关节炎; 清热除痹方; 作用机制; 信号转导通路
Keywords:
gout;  arthritis;  heat-clearing rheumatism-eliminating prescription;  mechanism;  signal pathway
分类号:
R684.3
文献标志码:
B
摘要:
目的：基于caspase 1/IL-1β信号转导通路探讨清热除痹方治疗痛风性关节炎(GA)的作用机制。方法：将57例患者随机分为对照组29例和观察组28例，对照组口服布洛芬缓释片0.6 g/次，1次/d，观察组在此基础上给予外敷及内服清热除痹方。采用ELISA双抗夹心法测定治疗前后2组外周血炎性因子白细胞介素1β(IL-1β)、白细胞介素8(IL-8)水平。采用实时荧光定量法PCR检测2组患者外周血单核细胞(PBMC)中Nod 样受体蛋白3(NLRP3)炎性体、半胱氨酸天冬氨酸酶1(Caspase-1)、相关斑点样蛋白(ASC)mRNA表达，Western blot检测ASC，Caspase-1信号蛋白表达。结果：2组PBMC中ASCmRNA 表达量、外周血炎性因子IL-1β、IL-8水平均明显低于治疗前，而caspase-1、NLRP3mRNA 表达量则明显高于治疗前(P＜0.05)；且观察组治疗后ASCmRNA表达量明显低于对照组(P＜0.05)；治疗后NLRP3mRNA及caspase-1mRNA表达量均明显高于对照组；观察组治疗后外周血IL-1β及IL-8水平均明显低于对照组；治疗后观察组ASC蛋白水平明显低于对照组(P＜0.05)。结论：清热除痹方对GA的抗炎效果更佳，可能与较好地调控caspase-1/IL-1β信号转导通路，抑制IL-1β等炎性因子的释放，缓解炎症症状有关。
Abstract:
Objective: To discuss the mechanism of heat-clearing rheumatism-eliminating prescription in the treatment for gouty arthritis (GA) based on caspase1//IL-1β signal pathway. Methods: All 57 patients were randomized into the control group (29 cases, oral administration of Ibumetin Retavd, 0.6g each time, once each day) and the observation group (29 cases, heat-clearing rheumatism-eliminating prescription for external use+oral administration based on the therapy the control group accepted), ELISA double antibody sandwich method was used to detect the levels of IL-8 and IL-1β in peripheral blood of both groups before and after treating. Real-time fluorescence quantitative method was adopted to detect the expressions of ASC mRNA, caspase-1, NLRP3 inflammatory body of PBMC in peripheral blood between both groups, Western blot was adopted to detect the expressions of ASC and caspase-1 signal protein. Results: The expressions of ASC mRNA in PBMC, the levels of IL-8 and IL-1β in peripheral blood were lower than these before treating in both groups, while the expressions of caspase-1 and NLRP3mRNA were higher than these before treating (P＜0.05); and ASCmRNA expressions of the observation group after treating were lower than these of the control group notably (P＜0.05); while after treating, the expressions of NLRP3mRNA and caspase-1mRNA were higher than these of the control group notably; the levels of IL-8 and IL-1β in peripheral blood of the observation group after treating were lower than these of the control group notably, after treating, ASC protein level of the observation group was lower than that of the control group remarkably (P＜0.05). Conclusion: Heat-clearing rheumatism-eliminating prescription owns the best inflammatory effects for GA, and it might be related to regulating caspase1//IL-1β signal pathway, inhibiting the release of inflammatory factors including IL-1β and others and relieving inflammatory symptoms.

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备注/Memo

备注/Memo:
收稿日期：2018-09-15*基金项目：广州市科技计划项目(编号201607010122)。作者简介：曹诗丹(1987—)，女，硕士学位。研究方向：风湿病的临床诊治。△通讯作者：徐伟(1971—)，女，博士学位，副主任医师。研究方向：风湿病的临床诊治。
更新日期/Last Update: 2019-08-15