基于群体效应研究双氢杨梅树皮素对金黄色葡萄球菌的体内抗菌作用-《西部中医药》

文章信息/Info

Title:: In-vivo Antibacterial Effects of Ampelopsis on Staphylococcus Aureus Based on Quorum Sensing

Author(s):: HONG Zhengshan^1,2, WANG Yuanxia¹, YANG Ke¹, ZENG Chunhui¹; 1.Guangxi University of Chinese Medicine, Nanning 530200, China
2.Guangxi International Zhuang Medicine Hospital

Keywords:: SA; pathogenicity; APS; in-vivo antibacterial effects; quorum sensing

摘要:: 目的探讨双氢杨梅树皮素（ampelopsis，APS）通过群体效应(quorum sensing，QS)对体内金黄色葡萄球菌（staphylococcus aureus，SA）的抗菌作用。方法对标准SA NTCC8325及其QS调控基因缺失株NTCC8325ΔagrA、NTCC8325luxS进行生长曲线测定，以确定3个株菌的活力最强时期；检测高、中、低剂量APS给药后3个株菌腹腔感染小鼠腹腔液活菌数和小鼠血液白细胞数。结果标准SANTCC8325活力最强时期为10～12 h，NTCC8325ΔagrA、NTCC8325ΔluxS活力最强时期为12～14 h；APS对3个株菌腹腔感染小鼠均有保护作用，且在一定范围内呈量效关系；APS对NTCC8325ΔluxS腹腔感染小鼠的保护作用较APS对NTCC8325ΔagrA腹腔感染小鼠的保护作用强。结论APS在体内通过直接影响agr QS系统及间接影响lux QS系统，从而产生抗SA的作用。

Abstract:: ObjectiveTo explore antibacterial effects of APS on SA in vivo based on QS. MethodsThe growth curves of standard SA NTCC8325 and its QS regulatory gene delection strains NTCC8325ΔagrA and NTCC8325luxS were determined, to confirm the most vigorous period of three strains; to measure the number of viable bacteria in peritoneal fluid of mice with abdominal infection and the number of white blood cells after administering with high, moderate and low dosages of APS. ResultsThe most vigorous period of standard SA NTCC8325 was from 10 to 12 hours, the periods of NTCC8325ΔagrA and NTCC8325luxS from 12 to 14 hours; APS owns protective effects on the mice suffering from three strains of bacteria infection in abdominal cavity, and it presented the dose-dependent relationship within a certain range; protective effects of APS on the mice with abdominal infection of NTCC8325ΔluxS were stronger compared with its effects on the mice with abdominal infection of NTCC8325ΔagrA. ConclusionAPS could produce anti-SA effects through directly affecting agr QS system and lux QS system indirectly in vivo.