[1]张万涛,李鸿,张钰,等.土家族药物豆板还阳的HPLC指纹图谱研究[J].西部中医药,2022,35(10):52-56.[doi:10.12174/j.issn.2096-9600.2022.10.11]
　ZHANG Wantao,LI Hong,ZHANG Yu,et al.Study on HPLC Fingerprint of Tujia Ethnic Medicine Douban Huanyang[J].Western Journal of Traditional Chinese Medicine,2022,35(10):52-56.[doi:10.12174/j.issn.2096-9600.2022.10.11]
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土家族药物豆板还阳的HPLC指纹图谱研究
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
35
期数:
2022年10期
页码:
52-56
栏目:
出版日期:
2022-10-15

文章信息/Info

Title:
Study on HPLC Fingerprint of Tujia Ethnic Medicine Douban Huanyang
作者:
张万涛1, 李鸿2,3, 张钰2,3, 郑露2,3, 涂星2,4
1.恩施土家族苗族自治州中心医院，湖北 恩施 445000
2.湖北民族大学医学部
3.风湿病发生与干预湖北省重点实验室
4.湖北民族大学武陵山中药材检验检测中心
Author(s):
ZHANG Wantao1, LI Hong2,3, ZHANG Yu2,3, ZHENG Lu2,3, TU Xing2,4
1.The Central Hospital of Enshi Tujia and Miao Autonomous Perfecture， Enshi 445000， China
2.Medical School， Hubei Minzu University
3.Hubei Provincial Key Laboratory of Occurrence and Intervention of Rheumatic Diseases
4.Wuling Mountain Chines
关键词:
豆板还阳; 吊石苣苔; 高效液相色谱法; 指纹图谱; 石吊兰素
Keywords:
HPLC; fingerprint; lysionotin
分类号:
R282
DOI:
10.12174/j.issn.2096-9600.2022.10.11
文献标志码:
A
摘要:
目的建立土家族药物豆板还阳的HPLC指纹图谱特征，为豆板还阳的真伪鉴别和质量控制提供参考。 方法采用Inertsustain C18色谱柱（4.6 mm×150 mm，5 μm），检测波长为332 nm，柱温30℃，乙腈（A）-0.1%磷酸水（B）为流动相，梯度洗脱：0～25 min，15% A；25～55 min，15%→52%A，流速1.0 mL/min，进样量10 μL。测定8批豆板还阳的指纹图谱，筛选其共有模式，标定共有指纹峰，采用中药色谱指纹图谱相似度评价系统（2012A版）软件计算相似度，以石吊兰素对照品指认色谱峰。 结果建立了土家族药物豆板还阳的HPLC指纹图谱，对8批样品的相似度进行了比较，标定了16个共有峰，并指认了16号峰为石吊兰素。 结论不同产地土家族药物豆板还阳主成分基本相同，但各组分含量的差别较大；本研究建立的方法简便快捷，重复性好，特征性强，可为豆板还阳的真伪鉴别、资源开发利用和质量控制提供参考。
Abstract:
ObjectiveTo provide the reference for true and false identification of Douban Huanyang (Lysionotus pauciflorus) and quality control by establishing the characteristics of HPLC fingerprints of the medicine. MethodsInertsustain C18 chromatographic column (4.6 mm×150 mm, 5 μm) was adopted, detection wavelength was 332 nm, column temperature 30℃, acetonitrile (A) -0.1% phosphate water (B) as mobile phase, gradient elution: 0～25 min, 15%A; 25～55 min, 15%→52%A, flow velocity: 1.0 mL/min, sample size was 10 μL. The fingerprint patterns of eight batches of Douban Huanyang were determined, to screen the common patterns, and calibrate the common fingerprint peaks, similarity evaluation system of chromatographic fingerprint of traditional Chinese medicine (2012A edition) was adopted to calculate the similarity, chromatographic peaks were identified with the reference substance of lysionotin. ResultsHPLC fingerprint of Douban Huanyang has been established, and the similarity of eight batches of the samples was compared, and the common fingerprint peaks were calibrated, meanwhile, peak No.16 has been identified as lysionotin. ConclusionThe principal components of Douban Huanyan from different producing areas are basically the same, while there are disparities between the contents of the components; the method established in the study, simple and convenient, rapid, reproducible and strong features, could provide the reference for true and false identification, resource exploitation and utilization, and quality control of Douban Huanyang.

备注/Memo

备注/Memo:
张万涛（1993—），男，主管中药师。研究方向：民族药物资源的挖掘、整理、开发与利用。湖北省教育科学规划重点课题（2016GA024）；湖北省大学生创新创业训练计划项目（S201910517032）。
更新日期/Last Update: 2022-10-15