[1]姚伟洁,赵香妍.木丹颗粒通过miR-146a调控对糖尿病周围神经病变大鼠TLR4通路的影响[J].西部中医药,2022,35(10):48-51.[doi:10.12174/j.issn.2096-9600.2022.10.10]
　YAO Weijie,ZHAO Xiangyan.Influence of Mudan Granules on TLR4 Signaling in DPN Rats Via miR-146a Regulation[J].Western Journal of Traditional Chinese Medicine,2022,35(10):48-51.[doi:10.12174/j.issn.2096-9600.2022.10.10]
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木丹颗粒通过miR-146a调控对糖尿病周围神经病变大鼠TLR4通路的影响
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
35
期数:
2022年10期
页码:
48-51
栏目:
出版日期:
2022-10-15

文章信息/Info

Title:
Influence of Mudan Granules on TLR4 Signaling in DPN Rats Via miR-146a Regulation
作者:
姚伟洁1, 赵香妍2
1.首都医科大学附属北京妇产医院北京妇幼保健院，北京 100006
2.北京市和平里医院
Author(s):
YAO Weijie1, ZHAO Xiangyan2
1.Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing Maternal and Child Health Care Hospital, Beijing 100006, China
2.Beijing Hepingli Hospital
关键词:
糖尿病周围神经病变; 雪旺细胞; 木丹颗粒; miR-146a; TLR4通路; 动物实验
Keywords:
diabetic peripheral neuropathy; Schwann cells; granules; miR-146a; TLR4 signaling; animal experiment
分类号:
R587.2
DOI:
10.12174/j.issn.2096-9600.2022.10.10
文献标志码:
A
摘要:
目的探讨木丹颗粒通过miR-146a调控对糖尿病周围神经病变大鼠TLR4通路的影响。 方法1）动物实验。40只SD大鼠中，设10只为空白对照组，其余制备糖尿病模型大鼠后分为模型组，α-硫辛酸组和木丹颗粒组，每组10只。灌胃给予相应药物，12周后处死并收集坐骨神经。2）细胞实验。将RSC96细胞分为25、150 mM组，150 mM+α-硫辛酸组和150 mM+木丹颗粒（10%，1%和0.1%）组，分别给予相应含药血清孵育48 h后，收集细胞。3）RT-PCR法测定坐骨神经和RSC96细胞的miR-146a表达，RT-PCR法和Western blot法测定坐骨神经和RSC96细胞中TRAF6和IRAK1 mRNA及蛋白表达。 结果动物实验中，与空白对照组比较，模型组miR-146a表达显著降低（P＜0.01），IRAK1和TRAF6 mRNA及蛋白表达均显著升高（P＜0.01）；与模型组比较，木丹颗粒组miR-146a表达显著升高（P＜0.01），IRAK1和TRAF6 mRNA及蛋白表达均显著降低（P＜0.01）。细胞实验中，与25 mM组比较，150 mM组miR-146a表达显著降低（P＜0.01），IRAK1和TRAF6 mRNA及蛋白表达均显著升高（P＜0.01）；与150 mM组比较，木丹颗粒含药血清组miR-146a表达显著升高（P＜0.01），10%和1%木丹颗粒含药血清组IRAK1和TRAF6 mRNA及蛋白表达均显著降低（P＜0.01、P＜0.05）,0.1%木丹颗粒含药血清组IRAK1 mRNA表达显著降低（P＜0.05）。 结论木丹颗粒通过促进miR-146a的表达，抑制IRAK1和TRAF6的表达，从而调控TLR4相关通路，抑制炎症反应，防治糖尿病周围神经病变。
Abstract:
Objective: To discuss the mechanism of Mudan granules influencing TLR4 signaling in rats with diabetic peripheral neuropathy (DPN) via miR-146a regulation. Methods1) Animal experiments. Ten rats were chosen as blank control group from forty SD ones, the remaining were prepared into the models with diabetes mellitus (DM), and randomized into the model group,α-lipoic acid group and Mudan granules group, ten rats in each group. All the rats accepted gavage administration of the corresponding medicine. Sciatic nerves were coll-ected when they were sacrificed after 12 weeks. 2) Cell experiment. RSC 96 cells were divided into 25 and 150 mM groups, 150 mM+α-lipoic acid group and 150 mM+Mudan granules (10%, 1% and 0.1%) groups, and the cells were collected after giving them the corresponding drug-containing serum and incubating for 48 h. 3)RT-PCR method was used to detect the expressions of miR-146a in sciatic nerves and RSC96 cells, RT-PCR method and Western blot method were applied to measure the expressions of TRAF6 and IRAK1mRNA and protein in sciatic nerves and RSC96 cells. ResultsIn animal experiments, compared with blank control group, the expressions of miR-146a were decreased notably in the model group (P<0.01), the expressions of IRAK1 and TRAF6 mRNA and protein were raised remarkably (P<0.01); compared with the model group, the expressions of miR-146a in Mudan granules group were lifted (P<0.01), the expressions of IRAK1 and TRAF6 mRNA and protein were decreased significantly (P<0.01); in cell experiments, compared with 25 mM group, the expression of miR-146a in 150mM group was noticeably decreased (P<0.01), the expressions of IRAK1 and TRAF6 mRNA and protein were increased significantly (P<0.01); compared with 150 mM group, the expressions of miR-146a in Mudan granules-containing serum group were noticeably improved (P<0.01), the expressions of IRAK1 and TRAF6 mRNA and protein were decreased markably in 10% and 1% Mudan granules-containing serum groups (P<0.01, P<0.05), the expression of IRAK1 mRNA in 0.1% Mudan granules-containing serum group was reduced (P<0.05). ConclusionMudan granules could inhibit inflammatory reaction, prevent and treat DPN via promoting the expressions of miR-146a, inhibiting the expressions of IRAK1 and TRAF6, thereby regulating TLR4 associated signaling.

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备注/Memo

备注/Memo:
姚伟洁（1990—），男，药师。研究方向：糖尿病周围神经病变的中药复方治疗及机制研究。北京市自然科学基金（7194268）。
更新日期/Last Update: 2022-10-15