[1]田红艳,许蕊蕊.不同产地葛根微乳提取物对链脲佐菌素2型糖尿病小鼠的降血糖作用探讨[J].西部中医药,2023,36(05):6-11.[doi:10.12174/j.issn.2096-9600.2023.05.02]
　TIAN Hongyan,XU Ruirui.Study on Hypoglycemic Activity of Microemulsion Extract of Gegen from Different Habitats on Streptozotocin- induced Type Two Diabetes Mellitus Mice[J].Western Journal of Traditional Chinese Medicine,2023,36(05):6-11.[doi:10.12174/j.issn.2096-9600.2023.05.02]
点击复制

不同产地葛根微乳提取物对链脲佐菌素2型糖尿病小鼠的降血糖作用探讨
分享到：

《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
36
期数:
2023年05期
页码:
6-11
栏目:
出版日期:
2023-05-15

文章信息/Info

Title:
Study on Hypoglycemic Activity of Microemulsion Extract of Gegen from Different Habitats on Streptozotocin- induced Type Two Diabetes Mellitus Mice
作者:
田红艳1, 许蕊蕊2
1.北京市怀柔区中医医院，北京 101400
2.北京中医药大学中药学院，北京 102488
Author(s):
TIAN Hongyan1, XU Ruirui2
1.Beijing Huairou Hospital of Traditional Chinese Medicine, Beijing 101400, China
2.School of Chinese Materia Medica of Beijing University of Chinese Medicine, Beijing 102488, China
关键词:
2型糖尿病; 微乳超声提取; 不同产地; 葛根
Keywords:
type two diabetes mellitus; microemulsion ultrasounic extraction; different habitats
分类号:
R255.4
DOI:
10.12174/j.issn.2096-9600.2023.05.02
文献标志码:
A
摘要:
目的优化微乳超声提取葛根中总黄酮和总多糖的最佳条件，比较不同产地葛根微乳提取物对链脲佐菌素（streptozotocin，STZ）引起的2型糖尿病（type 2 diabetes mellitus，T2DM）小鼠的治疗作用。 方法以总黄酮和总多糖的提取率综合评分为指标，采用星点设计法优化微乳超声提取的最佳条件，通过STZ诱导建立T2DM小鼠模型，以二甲双胍为阳性对照药，对比湖南、陕西、湖北和山西地区葛根对T2DM小鼠的降血糖作用。记录各组小鼠第0、4、8、14天非空腹血糖值，第1、2、4、6、8、10、12、14天进食量，第0、2、4、6、8、10、12、14天体质量，并观察小鼠大小便和精神状态。 结果微乳提取的最佳条件为溶剂物料比：6.8倍，提取时间：68 min，提取功率：520 W；湖北地区葛根总黄酮和总多糖提取率最高，分别为4.40%和8.50%。湖北地区葛根微乳对T2DM小鼠的治疗效果最佳。 结论该微乳超声提取工艺稳定、操作简便、提取率高，且湖北地区葛根微乳提取物所含总黄酮和总多糖高于其他3个产地，其对降低STZ致T2DM小鼠血糖的作用最显著。
Abstract:
ObjectiveTo optimize the optimal conditions for ultrasonic extraction of total flavonoids and total polysaccharides from Gegen puerariae lobatae radix by microemulsion ultrasound, and compare therapeutic effects of microemulsion extract of Gegen from different habitats on STZ-induced T2DM mice. MethodsBy taking comprehensive scores of yield rate for total flavonoids and total polysaccharides as the indexes, central composite design was applied to optimize the best conditions of microemulsion ultrasounic extraction, T2DM mice models were established by STZ induction, to compare hypoglycemic activity of Gegen from Hunan, Shaanxi, Hubei and Shanxi on T2DM mice by selecting melbine as positive control medicine. To record non-fast blood glucose values on the zero, forth, eighth and fourteenth day, food intakes on the first, second, forth, sixth, eighth, tenth, twelfth and fourteenth day, body mass on the zero, second, forth, sixth, eighth, tenth, twelfth and fourteenth day of the mice in all the groups, to observe the urination, defecation and mental states of the mice. ResultsThe optimal conditions for microemulsion extraction is solvent to material ratio:6.8 times, extraction time was 68 min, extraction power: 520 W; the extraction percentages of total flavonoids and total polysaccharides from Gegen of Hubei were the highest, 4.40% and 8.50% respectively. Microemulsion of Gegen of Hubei presents the best therapeutic effects on T2DM mice. ConclusionThe microemulsion ultrasounic extraction process is stable, and easy to operate, showing high extraction rate, the contents of total flavonoids and total polysaccharides from Gegen of Hubei are higher than these of other three regions, and they present the most remarkable effects on reducing blood glucose of T2DM mice induced by STZ.

相似文献/References:

[1]祁瑞芳.同型半胱氨酸、超敏 C- 反应蛋白在 2 型糖尿病合并脑梗死预测中的作用[J].西部中医药,2014,27(08):152.
[2]邱勇玉,李应东,赵信科,等.高血压病中医证型与 2 型糖尿病及其相关危险因素分析[J].西部中医药,2014,27(09):75.
[3]李东峰,指导：王志刚.王志刚主任医师治疗2型糖尿病“三级”防治思想辨治心得[J].西部中医药,2015,28(01):29.
[4]周雨聪,指导：刘召.红灵五黄汤治疗2型糖尿病90例[J].西部中医药,2015,28(04):99.
[5]杨国军.黄芪注射液对2型糖尿病患者血管功能及微循环状态的影响[J].西部中医药,2015,28(05):109.
[6]康学东,张瀚文.病证结合探讨2型糖尿病胰岛素抵抗[J].西部中医药,2015,28(11):112.
[7]张喜芬,赵保礼,杨立波,等.通络明目胶囊治疗糖尿病视网膜病变的随机双盲对照研究[J].西部中医药,2013,26(11):88.
　ZHANG Xifen,ZHAO Baoli,YANG Libo,et al.A Randomized, Double-blind and Placebo-controlled Study of TongLuo MingMu Capsule in Treating Diabetic Retinopathy[J].Western Journal of Traditional Chinese Medicine,2013,26(05):88.
[8]戴梅.全面干预护理对2型糖尿病患者血糖和糖化血红蛋白水平的影响[J].西部中医药,2013,26(07):109.
　DAI Mei.Intervention of Comprehensive Nursing on Blood Glucose and Glycosylated Hemoglobin of Patients with Diabetes Mellitus Type Two[J].Western Journal of Traditional Chinese Medicine,2013,26(05):109.
[9]张家林,裴瑞霞.银花痛风颗粒治疗2型糖尿病合并高尿酸血症疗效观察[J].西部中医药,2014,27(01):106.
　ZHANG Jialin,PEI Ruixia.Clinical Observation on YinHua TongFeng Granules in Treating Type 2 Diabetes Mellitus Complicated with Hyperuricemia[J].Western Journal of Traditional Chinese Medicine,2014,27(05):106.
[10]祁瑞芳.同型半胱氨酸、超敏C-反应蛋白在2型糖尿病合并脑梗死预测中的作用[J].西部中医药,2014,27(04):136.
　QI Ruifang.Diagnostic Value of Homocysteine and High Sensitivity C-Reactive Protein in Predicting Type 2 Diabetes Mellitus Complicated with Cerebral Infarction[J].Western Journal of Traditional Chinese Medicine,2014,27(05):136.

备注/Memo

备注/Memo:
田红艳（1974—），女，副主任中药师。研究方向：中药鉴定与临床中药学。国家中医药管理局全国中药特色技术传承人才培训项目［（2014）39］。
更新日期/Last Update: 2023-05-15