[1]李艳英,郭敏群,魏宏伟,等.左金方中吴茱萸药材UPLC指纹图谱的建立及药材质量评价[J].西部中医药,2023,36(12):7-11.[doi:10.12174/j.issn.2096-9600.2023.12.02]
　LI Yanying,GUO Minqun,WEI Hongwei,et al.Quality Evaluation of Wuzhuyu and Establishment of Its UPLC Fingerprint in Zuojin Prescription[J].Western Journal of Traditional Chinese Medicine,2023,36(12):7-11.[doi:10.12174/j.issn.2096-9600.2023.12.02]
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左金方中吴茱萸药材UPLC指纹图谱的建立及药材质量评价
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
36
期数:
2023年12期
页码:
7-11
栏目:
质量分析
出版日期:
2023-12-15

文章信息/Info

Title:
Quality Evaluation of Wuzhuyu and Establishment of Its UPLC Fingerprint in Zuojin Prescription
作者:
李艳英1,2, 郭敏群1, 魏宏伟3, 王洪成3, 杨秀伟2
1.北京盈科瑞创新医药股份有限公司，北京 102200
2.北京大学，北京 102200
3.盈科瑞（天津）创新医药研究有限公司，天津 300000
Author(s):
LI Yanying1,2, GUO Minqun1, WEI Hongwei3, WANG Hongcheng3, YANG Xiuwei2
1.Beijing Increasepharm Corporation Limited, Beijing 102200, China
2.Peking University, Beijing 102200, China
3.Increasepharm (Tianjin) Institute Co., Ltd, Tianjin 300000, China
关键词:
左金方; 吴茱萸; 指纹图谱; 含量测定; 主成分分析
Keywords:
prescription; fingerprint; content determination; principal component analysis
分类号:
R285
DOI:
10.12174/j.issn.2096-9600.2023.12.02
文献标志码:
A
摘要:
目的建立吴茱萸UPLC指纹图谱分析方法，对不同产地吴茱萸药材质量进行全面评价，为控制左金方中吴茱萸药材质量提供依据。 方法安捷伦Infinity 1290 Ⅱ高效液相色谱仪；Ultimate UHPLC AQ-C18色谱柱（2.1×100 mm，1.8 ?m），以乙腈（A）-0.01%三氟乙酸（B）梯度洗脱，流速为每分钟0.4 mL；检测波长为300 nm，进样量1 μL。采收集了17批不同产地吴茱萸样品进行指纹图谱分析，结合主成分分析及有效成分含量测定，对吴茱萸药材整体质量进行评价。 结果建立了吴茱萸药材UPLC指纹图谱，标定了14个共有峰。指纹图谱和主成分分析显示不同产地吴茱萸药材指纹图谱基本一致，化学成分种类基本相同，成分的相对含量在一定范围内波动。吴茱萸药材质量与果实成熟度有较大的相关性，成熟度较低的样品柠檬苦素含量较高，吴茱萸碱和吴茱萸次碱总量较低，而成熟度较高药材与之相反。 结论指纹图谱结合有效成分含量测定的方法可用于吴茱萸药材的整体质量评价，为左金方中吴茱萸药材质量控制提供依据。
Abstract:
ObjectiveTo provide the reference for quality control of Wuzhuyu (Euodiae fructus) in Zuojin prescription by establishing the method of analyzing UPLC fingerprint of Wuzhuyu and comprehensively assess the quality of Wuzhuyu from different producing areas. MethodsThe conditions for chromatography were Ultimate UHPLC AQ-C18 column (2.1 × 100 mm, 1.8 μm) with Agilent Infinity 1290 II HPLC, acetonitrile and 0.01% trifluoroacetic acid (TFA) solution as the mobile phase was used to perform gradient elution, with the velocity of 0.4 mL?min-1; detection wavelength was 300 nm, and the sample size was 1 μL. The overall quality of Wuzhuyu was assessed by fingerprint analysis of 17 batches of samples from different producing areas, combined with principal component analysis (PCA), and the content determination of the active ingredients. ResultsAll 14 common peaks were calibrated in UPLC fingerprint of Wuzhuyu. Fingerprint and PCA revealed that Wuzhuyu from different producing areas had the basically identical fingerprints, and the basically same sorts of chemical ingredients, while the relative content of the components fluctuated within a certain range. The quality of Wuzhuyu has a great correlation with the maturity of the fruit, the samples with lower maturity had a higher content of limonin, and a lower total amount of evodiamine and rutecarpine, whereas the opposite was true for higher maturity samples. ConclusionFingerprint in combination with the content determination of the active ingredients could be used for the evaluation of overall quality of Wuzhuyu, and it could provide the reference for quality control of Wuzhuyu in Zuojin prescription.

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[5]赵琦,王庆伟,张瑞涛,等.HPLC同时测定吴茱萸中吴茱萸碱、吴茱萸次碱和柠檬苦素含量的方法[J].西部中医药,2020,33(02):30.[doi:10.12174/j.issn.1004-6852.2020.02.08]
　ZHAO Qi,WANG Qingwei,ZHANG Ruitao,et al.Contents Determination of Evodiamine, Rutacarpine and Limonin of Wuzhuyu by HPLC Simultaneously[J].Western Journal of Traditional Chinese Medicine,2020,33(12):30.[doi:10.12174/j.issn.1004-6852.2020.02.08]

备注/Memo

备注/Memo:
李艳英（1979—），女，硕士学位，高级工程师。研究方向：中药研究。国家重点研发计划项目（2018YFC1704506）。
更新日期/Last Update: 2023-12-15