[1]皇甫海全,于海睿.基于RhoA/Rock1信号通路探讨养心汤保护血管内皮的作用机制[J].西部中医药,2024,37(12):16-21.[doi:10.12174/j.issn.2096-9600.2024.12.04]
　HUANGFU Haiquan,YU Hairui.Study on the Mechanism of Heart-nourshing Decoction in Protecting Vascular Endothelium Based on RhoA/Rock1 Signaling Pathway[J].Western Journal of Traditional Chinese Medicine,2024,37(12):16-21.[doi:10.12174/j.issn.2096-9600.2024.12.04]
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基于RhoA/Rock1信号通路探讨养心汤保护血管内皮的作用机制
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
37
期数:
2024年12期
页码:
16-21
栏目:
基础研究
出版日期:
2024-12-15

文章信息/Info

Title:
Study on the Mechanism of Heart-nourshing Decoction in Protecting Vascular Endothelium Based on RhoA/Rock1 Signaling Pathway
作者:
皇甫海全, 于海睿
上海中医药大学深圳医院/深圳市罗湖区中医院，广州 深圳 518133
Author(s):
HUANGFU Haiquan, YU Hairui
Shenzhen Hospital of Shanghai University of Traditional Chinese Medicine/Luohu District Hospital of TCM, Shenzhen518133, China
关键词:
动脉粥样硬化; 血管内皮损伤; 养心汤; RhoA/Rock1信号通路
Keywords:
atherosclerosis; vascular endothelial damage; heart-nourishing decoction; RhoA/Rock 1 signaling pathway
分类号:
R259
DOI:
10.12174/j.issn.2096-9600.2024.12.04
文献标志码:
A
摘要:
目的观察养心汤对SD模型大鼠血管内皮损伤的影响并探讨其作用机制。 方法将40只SD雄性大鼠按随机数字表法分为空白组、模型组、法舒地尔组、养心汤组，每组10只。空白组喂养普通饲料，模型组、法舒地尔组、养心汤组喂养含3%Ｌ-蛋氨酸的普通饲料共12周，建立大鼠血管损伤模型。建模成功后，法舒地尔组以2.7 mg/（kg·d）剂量腹腔注射盐酸法舒地尔，养心汤组以2.34 g/（kg·d）剂量灌胃养心汤浸膏混悬剂，空白组和模型组大鼠灌胃等体积纯净水，各组均干预4周。干预结束后，大鼠麻醉称重并取材，苏木精-伊红染色法（hematoxylin-eosin staining，HE）观察大鼠胸主动脉血管内皮形态学改变情况；反转录聚合酶链式反应（reverse transcription-polymerase chain reaction，RT-PCR）检测大鼠胸主动脉RhoA、Rock1、eNOS mRNA的表达；蛋白免疫印迹法（Western Blot，WB）检测大鼠胸主动脉RhoA、Rock1、p-MYPT1、eNOS蛋白表达。 结果HE染色结果显示，空白组大鼠血管内皮完整、连续，未见明显损伤；模型组大鼠血管内皮大部分脱落，偶见残存内皮，损伤程度明显加重；养心汤组、法舒地尔组大鼠血管内皮基本连续、完好，损伤程度明显减轻。RT-PCR、WB结果显示，养心汤对RhoA/Rock1信号通路的蛋白及基因表达均有下调作用（P＜0.05），同时能够上调eNOS蛋白及基因的表达（P＜0.05）。 结论养心汤能够改善血管内皮损伤模型大鼠病理损伤状态，其作用机制可能与下调大鼠胸主动脉RhoA、Rock1蛋白及mRNA表达，抑制RhoA/Rock1信号通路相关。
Abstract:
ObjectiveTo observe the influence of heart-nourishing decoction on vascular endothelial damage in SD rat models and discuss its mechanism. MethodsAll 40 SD male rats were allocated to the blank group, the model group, fasudil group and heart-nourishing decoction group according to random number table method with ten in each group. Normal diet was given to the blank group, the model group, fasudil group and heart-nourishing decoction group were fed normal diet containing 3% L-methionine, for twelve weeks, in order to establish rat models with vascular endothelial damage. After successfully modeling, fasudil group received fasudil hydrochloride intraperitoneally at a dose of 2.7 mg/(kg·d), heart-nourishing decoction accepted intragastric administration of the extract suspension of the decoction at a dose of 2.34 g/(kg·d), the blank group and the model group were perfused with equivalent volume of pure water, they were intervened for four weeks. By the end of the intervention, rats were anaesthetised, weighed and sampled, HE method was used to observe the morphological changes of the vascular endothelium of the thoracic aorta in rats; RT-PCR was applied to detect the expressions of RhoA, Rock1 and eNOS mRNA in thoracic aorta in rats; WB was utilized to measure the expressions of RhoA, Rock1, p-MYPT1 and eNOS protein in rat thoracic aorta. ResultsHE staining results displayed that the vascular endothelium was intact and continuous, with no obvious damage, most of the vascular endothelium was detached in the model group, with occasional remnants of endothelium, and the degree of injury was significantly aggravated. The vascular endothelium of rats in the heart-nourishing decoction and fasudil group was basically continuous and intact, and the degree of injury was apparently reduced. The results of RT-PCR and WB showed that the decoction could downregulate the expressions of protein and genes of RhoA/Rock1 signaling pathway (P<0.05), meanwhile it could up regulate the expressions of eNOS protein and gene (P<0.05). ConclusionThe decoction could improve pathological damage of the rat models suffering vascular endothelial damage, and its mechanism might be related to the reduction of RhoA, Rock1 protein and mRNA expression in thoracic aorta and the inhibition of RhoA/Rock1 signaling pathway.

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备注/Memo

备注/Memo:
皇甫海全（1989—），男，博士学位，主治医师。研究方向：中医药防治心脑血管疾病的临床及机制研究。2023年深圳市罗湖区软科学研究计划项目（LX202302068，LX202302082）。
更新日期/Last Update: 2024-12-15