[1]唐春丽,农必华,冯茵怡,等.四妙凉血颗粒5种成分含量测定及对急性痛风性关节炎大鼠的抗炎作用研究[J].西部中医药,2025,38(02):1-5.[doi:10.12174/j.issn.2096-9600.2025.02.01]
　TANG Chunli,NONG Bihua,FENG Yinyi,et al.Determination of the Contents of Five Components in Simiao Liangxue Granules and Its Anti-inflammatory Effects on Acute Gouty Arthritis Rats[J].Western Journal of Traditional Chinese Medicine,2025,38(02):1-5.[doi:10.12174/j.issn.2096-9600.2025.02.01]
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四妙凉血颗粒5种成分含量测定及对急性痛风性关节炎大鼠的抗炎作用研究()
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
38
期数:
2025年02期
页码:
1-5
栏目:
基础研究
出版日期:
2025-02-15

文章信息/Info

Title:
Determination of the Contents of Five Components in Simiao Liangxue Granules and Its Anti-inflammatory Effects on Acute Gouty Arthritis Rats
作者:
唐春丽1, 农必华1, 冯茵怡1, 林忆龙1, 魏江存2, 罗远1
1.广西中医药大学第一附属医院，广西 南宁 530023
2.广西中医药大学附属国际壮医医院，广西 南宁 530201
Author(s):
TANG Chunli1, NONG Bihua1, FENG Yinyi1, LIN Yilong1, WEI Jiangcun2, LUO Yuan1
1.The First Affiliated Hospital of Guangxi University of Chinese Medicine, Nanning 530023, China
2.Guangxi International Zhuang Medicine Hospital, Nanning 530201, China
关键词:
急性痛风性关节炎; 四妙凉血颗粒; 白细胞介素1; 白细胞介素6; 肿瘤坏死因子
Keywords:
acute gouty arthritis; granules; IL-1; IL-6; TNF-
分类号:
R259
DOI:
10.12174/j.issn.2096-9600.2025.02.01
文献标志码:
A
摘要:
目的建立四妙凉血颗粒中5种成分的含量测定方法，探讨四妙凉血颗粒对急性痛风性关节炎（acute gouty arthritis，AGA）大鼠的抗炎作用机制。 方法采用高效液相色谱法测定四妙凉血颗粒中5种成分含量；大鼠足踝关节注射尿酸钠悬液诱导AGA，采用容积法检测足踝关节肿胀程度；采用酶联免疫吸附试验法测定大鼠血清及关节液中白细胞介素1β（interleukin-1β，IL-1β）、IL-6和肿瘤坏死因子α（tumor necrosis factor α，TNF-α）含量；采用免疫组织化学和图像分析方法观测大鼠踝关节软骨组织中TNF-α蛋白表达水平。 结果方法学考察结果显示各项指标符合规定，四妙凉血颗粒样品中落新妇苷、盐酸小檗碱、山柰酚、黄芩素和汉黄芩素的平均回收率为97.14%～102.67%，RSD值均小于3.0%。尿酸钠混悬液注射后，与空白对照组比较，模型组不同时间点大鼠足踝关节肿胀度，关节液中IL-1β、IL-6、TNF-α含量，血清IL-6水平及TNF-α蛋白表达均增高（P＜0.05）；空白对照组大鼠软骨细胞可见少量TNF-α蛋白表达。与模型组比较，阳性组（秋水仙碱0.65 mg/kg）及四妙凉血颗粒高、中、低剂量（6、3、1.5 g/kg）组大鼠第3～5天足踝关节肿胀度，关节液中IL-1β、IL-6、TNF-α含量及血清IL-6、TNF-α水平均降低（P＜0.05）；四妙凉血颗粒低、中、高剂量组大鼠TNF-α蛋白积分吸光度降低（P＜0.05）。与阳性组比较，四妙凉血颗粒高、中、低剂量组大鼠足踝关节肿胀度，关节液中IL-1β、IL-6、TNF-α含量及血清IL-6、TNF-α表达水平和关节组织TNF-α蛋白积分吸光度均变化不明显，差异无统计学意义（P＞0.05）。 结论本实验建立了四妙凉血颗粒含量测定方法，该方法专属性强、重复性好、准确度高，可用于四妙凉血颗粒样品中落新妇苷、盐酸小檗碱、山柰酚、黄芩素和汉黄芩素的含量测定；四妙凉血颗粒治疗AGA的作用机制可能与其降低关节组织炎症因子IL-1β、IL-6和TNF-α水平有关。
Abstract:
ObjectiveTo establish the determination methods for the contents of five components in Simiao Liangxue granules and to discuss the anti-inflammatory mechanism of the medicine on rats with acute gouty arthritis (AGA). MethodsHPLC was used to detect the contents of five components in the medicine; AGA rat models were prepared by injecting sodium urate suspension into foot and ankle, the swelling degree of ankle joint was detected by volume method; ELISA method was adopted to measure the contents of IL-1β, IL-6 and TNF-α in the serum and joint fluid; immunohistochemical and image analysis methods were utilized to observe the expressions of TNF-α protein in cartilage tissue of ankle. ResultsThe results of methodological examination showed that all the indicators conformed to the regulations, average recovery rates of astilbin, berberine hydrochloride, kaempferol, baicalein and scutellarin ranged between 97.14% and 102.67% in the samples of the medicine, RSD values were smaller than 3.0%. After the injection of sodium urate suspension, compared with blank control group, more significant swelling of ankle joint could be seen, the levels of IL-1β, IL-6 and TNF-α in the joint fluid, the expressions of IL-6 and TNF-α in the serum were elevated in the model group at different time points (P<0.05); small amounts of TNF-α protein expressions could been found in the blank control group. Compared with the model group, the swelling of ankle joints in the positive group (colchicin, 0.65 mg/kg), high, moderate and low doses groups of Simiao Liangxue granules (6, 3 and 1.5 g/kg) was alleviated from the third to the fifth day, the contents of IL-1β, IL-6 and TNF-α in joint fluid, the levels of IL-6 and TNF-α in the serum were reduced (P<0.05); TNF-α protein integral absorbance was lowered in the low, moderate and high doses groups of the medicine (P<0.05). Compared with the positive group, the swelling of ankle joints, the contents of IL-1β, IL-6 and TNF-α in joint fluid, the levels of IL-6 and TNF-α in the serum, as well as TNF-α protein integral absorbance in the joint tissue were changed insignificantly, and the difference had no statistical meaning (P>0.05). ConclusionThe determination method for the contents of the granules established in the study, exclusive, reproducible and accurate, could be used for the content determination of astilbin, berberine hydrochloride, kaemp-ferol, baicalein and scutellarin in the samples of the granules; the mechanism of treating AGA with the medicine may be related to the reduction in the levels of local inflammation factors IL-1β, IL-6 and TNF-α in the joints.

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备注/Memo

备注/Memo:
唐春丽（1980—），女，硕士学位，副主任中药师。研究方向：中药及其制剂质量分析。国家中医药管理局“十二五”中医药重点学科（民族药学）壮药学资质项目；全国中药特色技术传承人才培训项目（20184828005）；广西自然科学基金项目（2020GXNSFAA259059）；广西中医药大学高层次人才培育创新团队资助项目（2022A008）。
更新日期/Last Update: 2025-02-15