[1]林燕平,袁嘉尧,郭海威,等.基于网络药理学探讨绞股蓝治疗骨质疏松症的作用机制[J].西部中医药,2025,38(02):75-80.[doi:10.12174/j.issn.2096-9600.2025.02.17]
　LIN Yanping,YUAN Jiayao,GUO Haiwei,et al.Network Pharmacology-based Discussion on the Mechanism of Jiaogulan in the Treatment of Osteoporosis[J].Western Journal of Traditional Chinese Medicine,2025,38(02):75-80.[doi:10.12174/j.issn.2096-9600.2025.02.17]
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基于网络药理学探讨绞股蓝治疗骨质疏松症的作用机制()
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
38
期数:
2025年02期
页码:
75-80
栏目:
二次研究
出版日期:
2025-02-15

文章信息/Info

Title:
Network Pharmacology-based Discussion on the Mechanism of Jiaogulan in the Treatment of Osteoporosis
作者:
林燕平1, 袁嘉尧2,3, 郭海威1, 黄宏兴1, 陈桐莹2, 黄佳纯1, 姜涛4
1.广州中医药大学第三附属医院，广东 广州 510240
2.广州中医药大学第三临床医学院，广东 广州 510405
3.广州中医药大学，广东 广州 510006
4.广东省第二中医院，广东 广州 510095
Author(s):
LIN Yanping1, YUAN Jiayao2,3, GUO Haiwei1, HUANG Hongxing1, CHEN Tongying2, HUANG Jiachun1, JIANG Tao4
1.The Third Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 510240, China
2.The Third Clinical Medical School of Guangzhou University of Chinese Medicine, Guangzhou 510405, China
3.Guangzhou University of Chinese Medicine, Guangzhou 510006, China
4.Guangdong Second Traditional Chinese Medicine Hospital, Guangzhou 510095, China
关键词:
骨质疏松症; 绞股蓝; 作用机制; 网络药理学
Keywords:
osteoporosis; mechanism; network pharmacology
分类号:
R274.9
DOI:
10.12174/j.issn.2096-9600.2025.02.17
文献标志码:
A
摘要:
目的基于网络药理学方法探讨绞股蓝防治骨质疏松症（osteoporosis，OP）的有效成分及作用机制。 方法使用中药系统药理学数据库与分析平台（traditional Chinese medicine system pharmacology，TCMSP）筛选绞股蓝的活性成分和药物靶点；利用人类基因数据库（the human gene database，GeneCards）和在线人类孟德尔遗传数据库（online mendelian inheritance in man，OMIM）筛选OP相关靶点基因；对绞股蓝靶点和OP靶点取交集，通过Cytoscape软件建立绞股蓝-活性成分-靶点基因-OP网络；对绞股蓝和OP交集靶点进行蛋白-蛋白互作（protein-protein interactions，PPI）网络分析、基因本体论（gene ontology，GO）及京都基因与基因组百科全书（kyoto encyclopedia of genes and genomes，KEGG）富集分析。 结果筛选得到绞股蓝有效活性成分24种，药物靶点120个，与OP交集靶点87个；IL-6、AKT1、VEGFA、MAPK1等可能是绞股蓝防治OP的核心靶点；主要涉及核受体的活动、配体激活的转录因子活性、类固醇激素受体活性、细胞因子受体结合以及细胞因子活性等生物过程；共涉及AGE/RAGE、肿瘤坏死因子、IL-17、MAPK以及PI3K/AKT等信号通路。 结论绞股蓝防治OP具有多成分、多靶点、多通路特点，这些靶点和通路与骨代谢和骨重建有关。
Abstract:
ObjectiveTo explore the active ingredients and the mechanism of Jiaogulan [Gynostemma pentaphyllum (Thunb.) Makino] in the prevention and treatment of osteoporosis (OP) based on network pharmacology. MethodsTCMSP was applied to screen the active ingredients and the targets of Jiaogulan; GeneCards and OMIM were utilized to screen the genes of OP-related targets; the network of Jiaogulan-active ingredients- target genes-OP was constructed via Cytoscape software after intersecting the targets of Jiaogulan with OP targets; PPI network analysis, GO and KEGG enrichment analysis of Jiaogulan and OP intersecting targets were performed. ResultsThe study has yielded 24 active ingredients, 120 herbal targets of Jiaogulan, and 87 intersected targets of OP; IL-6, AKT1, VEGFA and MAPK1 might be the core targets of Jiaogulan in the prevention and treatment of OP; the study mainly referred to the biological processes of the activity of nuclear receptors, the activity of ligand-activated transcription factor, the activity of steroid hormone receptor, cytokine receptor binding and the activity of cytokine; involving AGE/RAGE, tumor necrosis factor, IL-17, MAPK and PI3K/AKT, and other signaling pathways. Conclusion Jiaogulan could prevent and treat OP via many ingredients, many targets and pathways, these targets and pathways are associated with bone metabolism and bony remodeling.

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备注/Memo

备注/Memo:
林燕平（1995—），女，医师。研究方向：骨质疏松症的中医药防治。国家自然科学基金（81973886，82374482）；广州市科技计划项目（202201011708）；广州中医药大学“双一流”与高水平大学学科协同创新团队重点项目（2021XK21）。
更新日期/Last Update: 2025-02-15