[1]陈方飞,刘雅玲,罗幸,等.基于网络药理学探讨丹参-红花药对治疗糖尿病周围神经病变的作用机制[J].西部中医药,2025,38(04):47-52.[doi:10.12174/j.issn.2096-9600.2025.04.10]
　CHEN Fangfei,LIU Yaling,LUO Xing,et al.Network Pharmacology-based Exploration into the Mechanism of Danshen-Honghua Couplet Medicines in the Treatment of Diabetic Peripheral Neuropathy[J].Western Journal of Traditional Chinese Medicine,2025,38(04):47-52.[doi:10.12174/j.issn.2096-9600.2025.04.10]
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基于网络药理学探讨丹参-红花药对治疗糖尿病周围神经病变的作用机制()

《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
38
期数:
2025年04期
页码:
47-52
栏目:
二次研究
出版日期:
2025-04-15

文章信息/Info

Title:
Network Pharmacology-based Exploration into the Mechanism of Danshen-Honghua Couplet Medicines in the Treatment of Diabetic Peripheral Neuropathy
作者:
陈方飞1, 刘雅玲2, 罗幸2, 麦小丽2, 邓小敏1
1.广西中医药大学，广西 南宁 530001
2.广西中医药大学第一附属医院，广西 南宁 530023
Author(s):
CHEN Fangfei1, LIU Yaling2, LUO Xing2, MAI Xiaoli2, DENG Xiaomin1
1.Guangxi University of Chinese Medicine, Nanning 530001, China
2.The First Affiliated Hospital of Guangxi University of Chinese Medicine, Nanning 530023, China
关键词:
糖尿病周围神经病变; 丹参; 红花; 网络药理学
Keywords:
diabetic peripheral neuropathy; network pharmacology
分类号:
R285
DOI:
10.12174/j.issn.2096-9600.2025.04.10
文献标志码:
A
摘要:
目的运用网络药理学方法探讨丹参-红花药对治疗糖尿病周围神经病变（diabetic peripheral neuropathy，DPN）的作用机制。 方法通过中药系统药理学数据库与分析平台（traditional Chinese medicine systems pharmacology database and analysis platform，TCMSP）检索丹参和红花的活性成分及对应靶点，借助UniProt数据库对靶点进行规范化；通过人类基因数据库（the human gene database，GeneCards）、Disgenet、药物靶标数据库（therapeutic targetdatabase，TTD）、在线人类孟德尔遗传数据库（online mendelian inheritance in man，OMIM）获取DPN的相关靶点，并利用R语言筛选药物与疾病的交集靶点；通过STRING数据库对交集靶点进行蛋白-蛋白互作（protein-protein interactions，PPI）网络分析，并通过Bioconductor平台对交集靶点进行基因本体论（gene ontology，GO）功能富集分析和京都基因与基因组百科全书（kyoto encyclopedia of genes and genomes，KEGG）通路富集分析；通过Cytoscape软件构建丹参-红花药对治疗DPN的“活性成分-疾病靶点”可视化调控网络及PPI调控网络，借助CytoNCA插件筛选并提取出核心调控网络得到的核心活性成分及作用靶点。 结果获得丹参-红花药对73个活性成分，对应215个作用靶点，其中与疾病的交集靶点96个；筛选出槲皮素、山柰酚、木犀草素、丹参酮ⅡA等16个核心活性成分，核心作用靶点包括AKT1、IL-1β等20个；GO功能富集分析涉及2497个条目，主要影响转录因子活性、酶活性、受体活性及生物化学过程调控等过程，KEGG通路富集分析得到158条，涉及到糖尿病并发症中的AGE-RAGE、IL-17、TNF等信号通路。 结论丹参-红花药对通过槲皮素、山柰酚、木犀草素、丹参酮ⅡA等活性成分干预AGE-RAGE等信号通路，并作用于AKT1等靶点，从而发挥治疗DPN的作用。
Abstract:
ObjectiveTo discuss the mechanism of Danshen (Salviae miltiorrhizae radix et rhizoma)-Honghua (Carthami flos) couplet medicines in the treatment of diabetic peripheral neuropathy (DPN) using network pharma-cology. MethodsThe active ingredients and the corresponding targets of the two drugs were searched from TCMSP, and the standardization of the targets were performed via UniProt database; DPN - related targets were gained from GeneCards, Disgenet, TTD and OMIM, the intersecting targets of medicine and disease were screened utilizing R language; protein-protein interactions (PPI) network analysis of the intersection targets was conducted via STRING database, GO enrichment analysis and KEGG enrichment analysis of the intersection targets were performed via Bioconductor platform; Cytoscape software was utilized to construct visual control network and PPI regulation network of "active ingredients-disease targets" of the couplet medicines in the treatment of DPN, the core active ingredients and the targets of action of the core regulatory network were screened and extracted by CytoNCA plug-in. ResultsThe study has gained 73 active ingredients related to the couplet medicines, corresponding to 215 targets of action, among them, there were 96 targets intersected with disease; 16 core active ingredients including quercetin, kaempferol, luteolin, tanshinone II A and others were screened, and there were 20 core targets of action containing AKT1, IL-1β and others; GO functional enrichment analysis involved 2497 items, mainly affecting the regulation of transcription factor activity, enzyme activity, receptor activity and biochemical processes, KEGG pathway enrichment analysis has obtained 158 pathways, involving signaling pathways such as AGE-RAGE, IL-17 and TNF in diabetic complications. Conclusion Danshen-Honghua couplet medicines acts on the targets including AKT1 and others by intervention on AGE-RAGE signaling pathways via the active ingredients containing quercetin, kaempferol, luteolin, tanshinone II A and others, thereby treating DPN.

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备注/Memo

备注/Memo:
陈方飞（1993—），女，硕士学位。研究方向：糖尿病的中医药防治及代谢研究。广西医疗卫生适宜技术开发与推广应用项目（S2018105）；广西中医药大学自然科学基金面上项目（2020MS019）。
更新日期/Last Update: 2025-04-15