[1]邓 强,许 伟,李盛华,等.消肿止痛合剂对大鼠急性脊髓损伤后NOS及MMP-9表达的影响*[J].西部中医药,2012,25(10):40-43.
 DENG Qiang,XU Wei,LI Sheng-hua,et al.The Influence of XiaoZhong ZhiTong Mixture on Expressions of NOS and MMP-9 in Rats with Acute Spinal Injury[J].Western Journal of Traditional Chinese Medicine,2012,25(10):40-43.
点击复制

消肿止痛合剂对大鼠急性脊髓损伤后NOS及MMP-9表达的影响*
分享到:

《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
25
期数:
2012年10期
页码:
40-43
栏目:
出版日期:
2012-10-15

文章信息/Info

Title:
The Influence of XiaoZhong ZhiTong Mixture on Expressions of NOS and MMP-9 in Rats with Acute Spinal Injury
文章编号:
1004-6852(2012)10-0040-04
作者:
邓 强许 伟李盛华陈志龙
甘肃省中医院,甘肃 兰州 730050
Author(s):
DENG Qiang, XU Wei, LI Sheng-hua, CHEN Zhi-long
Gansu Province Hospital of Traditional Chinese Medicine, Lanzhou 730050, China
关键词:
急性脊髓损伤消肿止痛合剂细胞凋亡率NOSMMP-9
Keywords:
acute spinal cord injury XiaoZhong ZhiTong mixture apoptosis rate NOS MMP-9
分类号:
R651.21
文献标志码:
A
摘要:
目的:观察消肿止痛合剂对大鼠急性脊髓损伤后细胞凋亡率、一氧化氮合成酶(NOS)及基质金属蛋白酶-9(MMP-9)的影响,探讨消肿止痛合剂治疗急性脊髓损伤的作用机理。方法:40只SD大鼠随机分为4组:空白对照组(A)、空白模型组(B)、甲基强的松龙组(C)、消肿止痛合剂组(D)。A组不做任何处理,B组、C组、D组采用脊髓半横切法造模后,B组给予生理盐水灌胃;C组给予肌注甲基强的松龙(30 mg/kg);D组给予消肿止痛合剂,每日用量为成人等效剂量10倍,3次/d,术后第8日起,2次/d,剂量不变。造模后14天,TUNEL染色法检测细胞凋亡率,大鼠尾静脉采血,放免法检测NOS含量,免疫组化法测定MMP-9的表达。结果:D组动物脊髓损伤部位细胞凋亡率明显低于B组(P<0.01),NOS含量低于B型组(P<0.01),同时B组大鼠脊髓组织中MMP-9 表达明显高于D剂组(P<0.01)。结论:消肿止痛合剂对脊髓损伤具有保护作用,可能是通过降低动物脊髓损伤部位NOS,维持TIMPs/MMP-9平衡,抑制MMP-9表达,从而调节细胞凋亡,保护脊髓功能。
Abstract:
Objective: To explore mechanism of XiaoZhong ZhiTong mixture in the treatment of acute spinal cord injury by observing the effects of XiaoZhong ZhiTong mixture on apoptosis rate, NOS and MMP-9 of rats with acute spinal cord injury. Method: Forty rats were randomized into four groups: blank control group (A), blank model group (B), methylprednisolone group (C) and XiaoZhong ZhiTong mixture group (D). A remained unhandled. B, C and D were handled with spinal cord hemisection method. B received physiological saline through intragastric administration; C were administered with intramuscular methylprednisolone(30mg/kg); D were given with XiaoZhong ZhiTong mixture, daily dose were ten times of adult equivalent dosage, three times per day, then changed to twice each day at the eighth day after surgery in the same dose. By the 14th day after establishing the models, apoptosis rate was detected with TUNEL tincture, NOS content was tested with radioimmunity and expression of MMP-9 detected with immunohistochemistry after drawing blood from caudal vein of rat. Result: Apoptosis rate of D was remarkably lower than B (P<0.01), NOS content was lower than B(P<0.01), meanwhile expression of MMP-9 in B was higher than D significantly (P<0.01). Conclusion: XiaoZhong ZhiTong mixture could protect spinal cord, which might regulate apoptosis and protect function of spinal function by decreasing NOS of injured region, maintain the balance of TIMPs/MMP-9 and inhibit the expression of MMP-9.

参考文献/References:

[1] Casha S,Yu WR,Fehlings MG.Oligodendoglial apoptosis occurs along degenerating axons and is associated with FAS and p75 expression following spinal cord injury inthe rats[J].Neuroscience,2001,103(1):203-218.
[2] Beanie,M.S.Current evidence for apoptosis after spinal cord injury[J].J Neurotrauma,2000,17:915-926.
[3] 胥少汀,郭世绂.脊髓损伤基础与临床[M].2版.北京:人民卫生出版社,2002:200.
[4] 周华,刘华,黄坚.基质金属蛋白酶-9与脊髓损伤后脊髓水肿的关系[J].中国脊柱脊髓杂志.2007:17(1):59-61.
[5] Sirbulescu RF,Zupanc GK.Inhibition of caspase-3- mediated apoptosis improves spinal cord repair in aregeneration-competent vertebrate system[J].Neuroscience,2010,171(2):599-612.
[6] Hale AJ,Smith CA,Sutherland LC,et al.Apoptosis:mole cular regulation of cell death[J]. Eur J Biochem,1996,236(1):1-26.
[7] Kaptanoglu E,Caner HH,Surucu HS,et al.Effect of mexiletine on lipid peroxidation and early ultrastructural finding in experimental spinal cord injury[J].J Neurosurg,1999,91(2 supple):200-204.
[8] 胡永华,韦贵康,吴雪挺.急性脊髓损伤治疗的实验研究及进展[J].中医正骨,2006,18(11):64-66.
[9] Bruno G,Todor R,Lewis I,et al.Vascular extracelluar matrix remodeling in cerebral aneursms[J].J Neurosurg,1998,89(3):431-440.
[10] 刘华,廖维靖,杨万同,等.脑缺血再灌后基质金属蛋白酶-9的表达及对血脑屏障的破坏[J].中华物理医学与康复杂志,2004,26(4):203-205.
[11] 周华,刘华.脊髓损伤后MMP-9表达的时空分布及其意义[J].创伤外科杂志,2005,7(3):175-177.
[12] CM Watamabe,M Sakural,K Abe,et al.Induction of Cu/Zn superoxide dismutas and nitric oxide synthase-like immunoreactivities in rabbit spinal cord after tran sient ischemia[M].Brain Research,1996,732(1-2):69-74.
[13] 周初松,严兵,刘成龙,等.一氧化氮合酶调节伤段脊髓血流量的作用及其机制[J].中华创伤杂志,2000,16(7):414-415.
[14] 赵立,李涛,贾连顺.脊髓损伤后一氧化氮合酶活性变化的动态观察及其基因表达[J].中国矫形外科杂志,2000,7(12):1196-1198.
[15] 曹苏,陈瑾,沈施仁.脊髓急性损伤后NOS、NO水平的变化[J].中国临床医学,2007,14(4):569-571.

相似文献/References:

[1]赵萍.消肿止痛合剂对大鼠骨折愈合中VEGF表达的影响[J].西部中医药,2013,26(11):16.
 ZHAO Ping.Effects of XiaoZhong ZhiTong Mixture on VEGF Expressions of Rats in the Healing Course of Bone Fracture[J].Western Journal of Traditional Chinese Medicine,2013,26(10):16.
[2]尤从新,柴喜平,李盛华,等.消肿止痛合剂对膝关节置换术患者应激反应的影响[J].西部中医药,2017,30(10):86.
 YOU Congxin,CHAI Xiping,Li Shenghua,et al.The Influence of XiaoZhong ZhiTong Mixture on Stress Reaction of the Patients Undergoing Total Knee Arthroplasty[J].Western Journal of Traditional Chinese Medicine,2017,30(10):86.
[3]毕军伟,李盛华,刘红喜,等.消肿止痛合剂配合消定膏治疗急性创伤性三角纤维软骨复合体损伤疗效观察[J].西部中医药,2018,31(11):32.
 BI Junwei,LI Shenghua,LIU Hongxi,et al.Effects of XiaoZhong ZhiTong Mixture and XiaoDing Ointment on Acute Traumatic TFCC Injury[J].Western Journal of Traditional Chinese Medicine,2018,31(10):32.
[4]何志军,何元旭,刘涛,等.消肿止痛合剂药效学及急性毒性试验研究[J].西部中医药,2022,35(07):31.[doi:10.12174/j.issn.2096-9600.2022.07.08]
 HE Zhijun,HE Yuanxu,LIU Tao,et al.Study on Pharmacodynamics and Acute Toxicity Test of Xiaozhong Zhitong Mixture[J].Western Journal of Traditional Chinese Medicine,2022,35(10):31.[doi:10.12174/j.issn.2096-9600.2022.07.08]
[5]李金鹏,郭婕,何志军,等.陇中消肿止痛合剂在皮瓣修复软组织缺损术后的应用效果[J].西部中医药,2021,34(01):31.[doi:10.12174/j.issn.2096-9600.2021.01.08]
 LI Jinpeng,GUO Jie,HE Zhijun,et al.Application Effects of Longzhong Xiaozhong Zhitong Mixture after Skin Flap Repairment of Soft Tissue Defect[J].Western Journal of Traditional Chinese Medicine,2021,34(10):31.[doi:10.12174/j.issn.2096-9600.2021.01.08]
[6]姚兴璋,董春璇,蒋振兴,等.基于“络以辛为泄”理论探讨消肿止痛合剂治疗皮瓣术后微循环障碍的机制[J].西部中医药,2024,37(05):60.[doi:10.12174/j.issn.2096-9600.2024.05.10]
 YAO Xingzhang,DONG Chunxuan,JIANG Zhenxing,et al.Exploration into the Mechanism of Edema-relieving Pain-easing Mixture in the Treatment of Microcirculatory Disorder after Flap Surgery Based on the Theory of "the Collateral Drained by the Pungent Herbs"[J].Western Journal of Traditional Chinese Medicine,2024,37(10):60.[doi:10.12174/j.issn.2096-9600.2024.05.10]

备注/Memo

备注/Memo:
*基金项目:甘肃省科技厅项目(编号096RJZA041) 作者简介:邓强(1970—),男,硕士学位,副主任医师,甘肃省卫生厅领军人才,甘肃省中西结合学会骨科微创专业委员会秘书长。研究方向:脊柱疾患及脊髓损伤。
更新日期/Last Update: 1900-01-01