[1]李新翔,李雪嫣,桂曼曼,等.复芪止汗颗粒对小鼠免疫功能影响的实验研究*[J].西部中医药,2012,25(11):18-21.
 LI Xin-xiang,LI Xue-yan,GUI Man-man,et al.Experimental Study on the Effects of FuQi ZhiHan Granules on Immune Function of Mice[J].Western Journal of Traditional Chinese Medicine,2012,25(11):18-21.
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复芪止汗颗粒对小鼠免疫功能影响的实验研究*
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
25
期数:
2012年11期
页码:
18-21
栏目:
方药·药理研究
出版日期:
2012-11-15

文章信息/Info

Title:
Experimental Study on the Effects of FuQi ZhiHan Granules on Immune Function of Mice
文章编号:
1004-6852(2012)11-0018-04
作者:
李新翔1李雪嫣2桂曼曼2张李峰2程卫东2△胡燕2
1 白银市白银区公园路街道银水巷社区卫生服务站,甘肃 白银 730900; 2 兰州大学基础医学院中西医结合研究所
Author(s):
LI Xin-xiang1 LI Xue-yan2 GUI Man-man2 ZHANG Li-feng2 CHENG Wei-dong2△ HU Yan2
1 Community Health Service Center of Y inShuiXiang in Baiyin District, Baiyin 730900, China; 2 Institute of Integrated Chinese and W estern Medicine in Basic Medicine of Lanzhou University
关键词:
非特异性免疫特异性免疫复芪止汗颗粒黄芪颗粒
Keywords:
nonspecific immunity specific immunity FuQi ZhiHan granules HuangQi granules
分类号:
R285.5
文献标志码:
A
摘要:
目的:探讨复芪止汗颗粒对小鼠非特异性免疫和特异性免疫的调节作用。方法:取正常昆明种小鼠 40 只,雌雄各半,随机分为 4 组,正常对照组、环磷酰胺模型组、黄芪颗粒组和复芪止汗颗粒组,每组 10 只。腹腔注射环磷酰胺制备免疫低下小鼠模型。测定胸腺、脾脏质量并计算出小鼠胸腺指数、脾指数;腹腔巨噬细胞吞噬中性红法检测单核 - 巨噬细胞的吞噬功能;血清溶血素法测定体液免疫功能;脾淋巴细胞增殖实验法测定细胞免疫功能;乳酸脱氢酶释放法测定 NK 细胞杀伤活性;流式细胞法检测 T 淋巴细胞亚群的数量。结果:复芪止汗颗粒可显著提高被环磷酰胺抑制的小鼠胸腺指数和脾脏指数;使巨噬细胞吞噬功能明显增加;使血清溶血素水平增高;并能拮抗环磷酰胺对小鼠脾脏 T 淋巴细胞增殖、杀伤活性、T 细胞亚群数量产生的免疫抑制作用。结论:复芪止汗颗粒能增强环磷酰胺所致免疫功能低下小鼠非特异性免疫和特异性免疫功能。
Abstract:
Objective: To approach the accommodation effects of FuQi ZhiHan granules on nonspecific and specific immunity of mice. Method: Forty mice were randomly divided into four groups: control group, model group,groups of HuangQi granules and FuQi ZhiHan granules, ten mice each group. The models were established through injecting cyclophosphamide into abdominal cavity. The qualities of thymus gland and spleen were detected to calculate the indexes of thymus gland and spleen; phagocytosis of the macrophage was determined with neural red assay of peritoneal macrophage phagocytosis; humoral immunity was measured by adopting serum hemolysis; cellular immune function was detected with splenic lymphocyte proliferation; anti-NK cell activity was measured with lactate dehydrogenase release method; numbers of T lymphocyte subgroups were counted with flow cytometric method.Result: FuQi ZhiHan granules can significantly raise thymus gland index and spleen index; improve the phagocytosis of the macrophage; increase serum hemolysin; fight against immunosuppressive action aroused by cyclophosphamide which can promote T lymphocyte proliferation, anti-activity and the quantity of T lymphocyte subgroup. Conclusion:FuQi ZhiHan granules can strengthen nonspecific and specific immunity in mice which show lower immune function caused bycyclophosphamide.

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备注/Memo

备注/Memo:
收稿日期:2012-02-12 * 基金项目:甘肃省中医药科研项目(编号 GZK-2008-7) 作者简介:李新翔(1962—),男,副主任医师。研究方向:心血管疾病及糖尿病的诊治。 △通讯作者:程卫东(1963—),男,博士研究生导师,教授。研究方向:常见疾病的中西医结合研究。
更新日期/Last Update: 2012-11-15