[1]吴国琳,李天一△,余国友,等.复方玄驹胶囊对不育症大鼠精子质量及生殖激素的影响*[J].西部中医药,2013,26(02):12-14.
 WU Guolin,LI Tianyi,Y U Guoyou,et al.Clinical Effects of FuFang XuanJu Capsule on Quality of Sperm and Reproductive Hormone in Rats with Infertility[J].Western Journal of Traditional Chinese Medicine,2013,26(02):12-14.
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复方玄驹胶囊对不育症大鼠精子质量及生殖激素的影响*
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
26
期数:
2013年02期
页码:
12-14
栏目:
方药·药理研究
出版日期:
2013-02-15

文章信息/Info

Title:
Clinical Effects of FuFang XuanJu Capsule on Quality of Sperm and Reproductive Hormone in Rats with Infertility
文章编号:
1004-6852(2013)02-0012-03
作者:
吴国琳1李天一2△余国友1卢雯雯1
1 浙江大学医学院附属第一医院,浙江 杭州 310003;2 浙江中医药大学
Author(s):
WU Guolin1 LI Tianyi2△ Y U Guoyou1 LU Wenwen1
1 The First A ffiliated Hospital of Medical School of Zhejiang University, Hangzhou 310003, China; 2 Zhejiang Chinese Medical University
关键词:
不育症精子质量生殖激素一氧化氮复方玄驹胶囊
Keywords:
infertility sperm quality reproductive hormone nitric oxide (NO) FuFang XuanJu capsule
分类号:
R285.5
文献标志码:
A
摘要:
目的:研究中药复方玄驹胶囊对不育症大鼠精子质量、生殖激素和一氧化氮(NO)、一氧化氮合酶(NOS)的影响。方法:将50只Wistar雄性大鼠随机分为正常组,模型组(雷公藤多甙造模),复方玄驹胶囊高、低剂量组和阳性对照组5组,每组10只。正常组、模型组每日予生理盐水灌胃,阳性对照组用枸橼酸氯米芬胶囊,复方玄驹胶囊高、低剂量组分别予相应剂量复方玄驹胶囊灌胃,连续喂养30天后处死大鼠,检测精子 质量、血清生殖激素和NO、NOS含量。结果:模型组大鼠精子密度、精子活率及活动度均低于其他各组(P<0.05),而畸形率高于各组(P<0.05);阳性对照组畸形率和正常组相比有统计学差异(P<0.05)。模型组血清NO、NOS含量均高于其他各组(P<0.05);阳性对照组血清NO高于复方玄驹高剂量组(P<0.05);阳性对照组血清NOS高于正常组(P<0.05)。模型组大鼠血清T、FSH低于其他各组(P<0.05)。模型组LH与正常组和高剂量组相比有统计学差异(P<0.05)。结论:复方玄驹胶囊能够改善不育症大鼠精子质量,调节生殖激素,提高大鼠精子活动度,减少精子畸形率,从而提高生育能力。
Abstract:
Objective: To study the effects of FuFang XuanJu capsule on sperm quality, reproductive hormone,nitric oxide (NO) and nitric oxide synthase (NOS) in rats with infertility. Method: Fifty rats were separated into normal group, model group (established with tripterygium wilfordii), high and low dose groups of FuFang XuanJu capsule and positive control group, ten rats each group. Normal group and model group received lavage of physiological saline, positive control group took clomiphene citrate capsules, high and low dose groups of FuFangXuanJu capsule received lavage with corresponding dosage. Rats were sacrificed after feeding 30 days continuously.Sperm quality, reproductive hormone in the serum, contents of NO and NOS were detected. Result: Model group were lower than other groups in sperm density, viability and motility (P<0.05), while malformation rate higher than that of other groups (P<0.05); comparison between positive control group and normal group showed significantd ifference in malformation rate (P<0.05). Contents of NO and NOS in model group were higher than that of other groups (P<0.05); NO in the serum of positive control group was higher than that of high dosage group of FuFangXuanJu capsule (P<0.05); positive control group was higher than normal group in serum NOS (P<0.05). Modelgroup was lower than other groups in serum T, FSH (P<0.05). The comparisons among model group, normal groupand high dose group showed significant difference in LH (P<0.05). Conclusion: FuFang XuanJu capsule could improve sperm quality, regulate reproductive hormone, increase sperm motility and reduce deformity rate of spermin rats with infertility to raise reproductive ability.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2012-10-27 * 基金项目:浙江省计划生育委员会资助课题(编号 201005) 作者简介:吴国琳(1972—),男,博士学位,副主任医师。研究方向:免疫系统疾病的中西医结合诊治。 △通讯作者:李天一(1972—),女,博士学位,副教授。研究方向:免疫性疾病的中医药防治。
更新日期/Last Update: 2013-02-15