[1]赵文娟,袁嘉丽,陈静,等.益气涤痰化瘀方对哮喘小鼠气道炎症及Th1/Th2平衡的影响[J].西部中医药,2015,28(05):18-21.
点击复制

益气涤痰化瘀方对哮喘小鼠气道炎症及Th1/Th2平衡的影响
分享到:

《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
28
期数:
2015年05期
页码:
18-21
栏目:
方药·药理研究
出版日期:
2015-05-15

文章信息/Info

文章编号:
1004-6852(2015)05-0018-04
作者:
赵文娟袁嘉丽陈静邢海晶韩妮萍△
云南中医学院,云南 昆明 650500
关键词:
哮喘干扰素-γ白介素-4益气涤痰化瘀方
分类号:
R222.19
文献标志码:
A
摘要:
目的:探讨益气涤痰化瘀方对哮喘小鼠气道炎症及Th1/Th2平衡的调节作用。方法:将SPF级BALB/c雌性小鼠50只随机分为正常对照组、哮喘模型组,益气涤痰化瘀方低、中、高剂量组。采用卵清白蛋白(OVA)腹腔注射及雾化激发法构建哮喘模型,益气涤痰化瘀方干预治疗。观察各组小鼠行为学改变;制作肺组织病理切片行镜下观察;收集肺泡灌洗液(BALF)进行白细胞总数及分类计数;取小鼠血清以ELISA法检测IFN-γ、IL-4的变化。结果:1)与哮喘模型组比较,药物干预组哮喘症状有所减轻,肺组织炎性细胞浸润减轻;2)益气涤痰化瘀方中剂量组小鼠BALF中白细胞总数和嗜酸性粒细胞(EOS)、中性粒细胞(NEU)、淋巴细胞(LYM)分类计数较哮喘模型组减少(P<0.05);3)与哮喘模型组比较,益气涤痰化瘀方高、中剂量组小鼠血清IFN-γ浓度升高(P<0.05),IL-4浓度降低(P<0.05)。结论:益气涤痰化瘀方可对免疫细胞Th2优势所致的免疫失衡状态进行调节,恢复Th1/Th2平衡;益气涤痰化瘀方可能通过调节机体平衡而减轻哮喘急性气道炎性病变,从而起到防治哮喘的作用。

相似文献/References:

[1]向林华.中药联合必可酮雾化吸入治疗小儿咳嗽变异性哮喘30例临床观察[J].西部中医药,2013,26(03):97.
 XIANG Linhua.Clinical Observation on Treatment for 30 Cases of Infantile Cough Variant Asthma with Herbs and Atomized Becotide Spray for Inhalation[J].Western Journal of Traditional Chinese Medicine,2013,26(05):97.
[2]赵庆大,刘萍,蔡乐,等.两益汤对哮喘豚鼠引喘潜伏期及TNF-α、IL-4 水平的影响[J].西部中医药,2013,26(05):16.
 ZHAO Qingda,LIU Ping,CAI Le,et al.Effects of LiangY iTang on Incubation Period of Asthma and Levels of TNF-α and IL-4 in Asthmatic Guinea Pigs[J].Western Journal of Traditional Chinese Medicine,2013,26(05):16.
[3]拜福兰.中西药物联合治疗小儿咳嗽变异性哮喘45例[J].西部中医药,2013,26(09):78.
 BAI Fulan.Treatment for Children Suffering from Cough Variant Asthma with Integrative Medicine[J].Western Journal of Traditional Chinese Medicine,2013,26(05):78.
[4]李冬梅,杨志华,刘晓霞,等.止咳平喘汤治疗小儿咳嗽变异性哮喘32例[J].西部中医药,2014,27(04):102.
 LI Dongmei,YANG Zhihua,LIU Xiaoxia,et al.ZhiKe PingChuan Tang in Treating 32 Cases of Cough Variant Asthma in Children[J].Western Journal of Traditional Chinese Medicine,2014,27(05):102.
[5]李晗怡,李亚军△.定喘汤治疗哮喘急性发作期患儿120例[J].西部中医药,2014,27(10):90.
[6]沈毅韵,庄承△,刘小敏.平喘方治疗小儿热性哮喘(轻度)的临床研究[J].西部中医药,2017,30(08):71.
 SHEN Yiyun,ZHUANG Cheng,LIU Xiaomin.Clinical Study on PingChuan Prescription in the Treatment of Children with Mild Febrile Asthma[J].Western Journal of Traditional Chinese Medicine,2017,30(05):71.
[7]薛晓东,宋红霞△.消银汤对小鼠鼠尾表皮细胞分化及Th1/Th2型细胞因子的影响[J].西部中医药,2018,31(08):10.
 XUE Xiaodong,SONG Hongxia.Effects of XiaoYin Decoction on Th1/Th2 Type Cell Factors and Epidermal Cell Differentiation of the Tail of Psoriasis like Mice Model[J].Western Journal of Traditional Chinese Medicine,2018,31(05):10.
[8]王云方.益肾固本膏方对肾阳虚哮喘模型大鼠肾上腺皮质类固醇合成酶及糖皮质激素受体亚型的影响[J].西部中医药,2022,35(04):25.[doi:10.12174/j.issn.2096-9600.2022.04.07]
 WANG Yunfang.Effects of Yishen Guben Ointment on Adrenal Cortical Steroid Synthase and Glucocorticoid Receptor Subtypes in Rat Model with Kidney-yang Deficiency Pattern Asthma[J].Western Journal of Traditional Chinese Medicine,2022,35(05):25.[doi:10.12174/j.issn.2096-9600.2022.04.07]
[9]史锁芳,胡珀,胡念芬.基于“厥阴欲解时”理论评价乌梅丸治疗夜间哮喘的临床疗效[J].西部中医药,2022,35(06):103.[doi:10.12174/j.issn.2096-9600.2022.06.25]
 SHI Suofang,HU Po,HU Nianfen.Clinical Effects of Wumei Pills in the Treatment of Nocturnal Asthma Based on the Theory of "The Time of Jueyin Disease Easily Attacking"[J].Western Journal of Traditional Chinese Medicine,2022,35(05):103.[doi:10.12174/j.issn.2096-9600.2022.06.25]
[10]綦梅伶,王静,邵茜彤.平喘汤联合沙丁胺醇对哮喘性支气管炎患儿Rrs SO、Crs SO水平的影响[J].西部中医药,2022,35(07):79.[doi:10.12174/j.issn.2096-9600.2022.07.21]
 QI Meiling,WANG Jing,SHAO Xitong.Effects of Pingchuan Tang Combined with Salbutamol on the Levels of Rrs SO and Crs SO in Children with Asthmatic Bronchitis[J].Western Journal of Traditional Chinese Medicine,2022,35(05):79.[doi:10.12174/j.issn.2096-9600.2022.07.21]

备注/Memo

备注/Memo:
*基金项目:云南省基础研究重点项目(编号2013FA041);云南省应用基础研究面上项目(编号2011FZ156)。作者简介:赵文娟(1981—),女,硕士学位,计师。研究方向:中西医结合基础免疫学。△通讯作者:韩妮萍(1978—),女,硕士学位,副教授。研究方向:中西医结合基础免疫学。
更新日期/Last Update: 2015-05-15