甜叶菊中总多酚及总多糖含量测定-《西部中医药》

文章信息/Info

Title:: Content Determination of Total Polysaccharide and Total Polyphenols of TianYeJu

作者:: 何巧丽1，李柯翱2，季志红2，田树革3△; 1 新疆医科大学中医学院，新疆乌鲁木齐 830011； 2 新疆奇康哈博维药有限公司；3 新疆医科大学中心实验室

Author(s):: HE Qiaoli1, LI Keao2, JI Zhihong2, TIAN Shuge3△; 1 TCM College of Xinjiang Medical university, Urumqi, 830011, China; 2 Xinjiang QiKang HaBo Uygur Medicine Co., LTD; 3 Central Lab of Xinjiang Medical University

Keywords:: the extract of TianYeJu; total polyphenols; total polysaccharide; content determination

摘要:: 目的:建立测定甜叶菊提取物中总多酚和总多糖含量的方法，并测定其含量。方法:分别以没食子酸和葡萄糖作为测定总多酚和总多糖含量的对照品，采用可见分光光度法测定甜叶菊提取物中总多酚和总多糖的含量。结果:测得甜叶菊提取物中总多酚总多糖含量分别为1.36 mg、1.73 mg。结论:该方法简单可行，重现性良好，测定结果准确、可信，可以作为甜叶菊提取物中总多酚和总多糖含量的测定方法。

Abstract:: Objective: To establish the method of determining the contents of total polyphenols and total polysaccharide in the extract of TianYeJu[Stevia rebaudiana (Bertoni) Hemsl], and determine their contents. Methods: The contents of total polyphenols and total polysaccharide in the extract of TianYeJu were detected by visible spectrophotometric method, gallic acid and glucose were tanked as the control articles of determining total polyphenols and total polysaccharide. Results: The contents of total polyphenols and total polysaccharide in the extract of TianYeJu were 1.36 mg and 1.73 mg respectively. Conclusion: The method, simple, feasible, reproducible, accurate and reliable, could be taken as the method of determining the contents of total polyphenols and total polysaccharide in the extract of TianYeJu.