[1]李志浩,李鹏,周雪红,等.金银花多糖对BCG+LPS致小鼠免疫性肝损伤的保护作用[J].西部中医药,2019,32(03):14-16.
　LI Zhihao,LI Peng,ZHOU Xuehong,et al.Protective Effects of FLP-3 on Immunological Liver Injury Induced by BCG+LPS in Mice[J].Western Journal of Traditional Chinese Medicine,2019,32(03):14-16.
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金银花多糖对BCG+LPS致小鼠免疫性肝损伤的保护作用
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
32
期数:
2019年03期
页码:
14-16
栏目:
出版日期:
2019-03-15

文章信息/Info

Title:
Protective Effects of FLP-3 on Immunological Liver Injury Induced by BCG+LPS in Mice
文章编号:
1004-6852(2019)03-0014-03
作者:
李志浩，李鹏，周雪红，邵将，柯昌虎，冯协和，姜雪强
湖北医药学院附属东风医院药学部，湖北 十堰 442008
Author(s):
LI Zhihao, LI Peng, ZHOU Xuehong, SHAO Jiang, KE Changhu, FENG Xiehe, JIANG Xueqiang△
Department of Pharmacy, Affiliated Dongfeng Hospital, Hubei University of Medicine, Shiyan 442008, China
关键词:
免疫性肝损伤; 保护作用; 金银花多糖; 小鼠
Keywords:
immunological liver injury;  protective effects;  FLP-3;  mice
分类号:
R575
摘要:
目的：探讨金银花多糖(FLP-3)对卡介苗加脂多糖(BCG+LPS)致小鼠免疫性肝损伤的影响。方法：将72只昆明种小鼠按随机数字表法分为正常组、BCG+LPS免疫性肝损伤小鼠组(模型组)、对照组(联苯双酯)及FLP-3低、中、高剂量组，每组12只；制备小鼠免疫性肝损伤模型；观察各组小鼠肝组织Bcl-2、Bax阳性表达率、血清白细胞介素10(IL-10)、白细胞介素12(IL-12)水平和小鼠肝组织的病理改变。结果：与正常组比较，模型组小鼠肝组织Bax表达上升(P＜0.01)，Bcl-2表达降低(P＜0.01)；与模型组比较，FLP-3各剂量组Bax表达下降(P＜0.01)，Bcl-2表达上升(P＜0.01)，而FLP-3高剂量组与对照组肝组织Bax及Bcl-2表达比较差异无统计学意义(P＞0.05)。与正常组比较，模型组小鼠血清IL-10、IL-12水平升高(P＜0.01)；与模型组比较，FLP-3各剂量组IL-10、IL-12水平下降(P＜0.01)；与对照组比较，除FLP-3高剂量组血清IL-12水平差异无统计学意义外，其余剂量组血清IL-10、IL-12水平均降低(P＜0.05)。结论：金银花多糖对LPS+BCG致小鼠免疫性肝损伤有明显的保护作用，而防止肝细胞过度凋亡可能是其防治免疫性肝损伤的作用机制。
Abstract:
Objective: To discuss the effects of honeysuckle flos lonicera polysaccharide (FLP-3) on immuno-logical liver injury(ILI) induced by BCG+LPS in mice. Methods: All 72 mice were divided into six groups according to random number table method, that is, the normal group, BCG+LPS-induced ILI group(the model group), the control group (bifendate), low, moderate and high doses groups of FLP-3 12 mice each group; mice models suffering ILI were prepared; to observe pathological changes of liver tissue, the levels of IL-10 and IL-12, positive expression rates of Bcl-2 and Bax. Results: Compared with the normal group, the expressions of Bax in liver tissue of the model group rose (P＜0.01), the expression of Bcl-2 decreased (P＜0.01); compared with the model group, the expressions of Bax lowered in different doses groups of FLP-3 (P＜0.01), the expression of Bcl-2 rose (P＜0.01), while there was no significant difference in the comparisons of the expression of Bax and Bcl-2 when high dose group of FLP-3 was compared with the control group (P＞0.05). Compared with the normal group, the levels of IL-10 and IL-12 rose in the model group (P＜0.01); compared with the model group, the levels of IL-10 and IL-12 lowered in different doses groups of FLP-3 (P＜0.01); compared with the control group, the levels of IL-10 and IL-12 lowered in different doses groups except there was no statistical difference in the level of IL-12 in high dose group of FLP-3(P＜0.05). Conclusion: FLP-3 could protect the mice with BCG+LPS - induced ILI obviously, and preventing theexcessive apoptosis of liver cells might be the mechanism of FLP-3 preventing and treating ILI.

参考文献/References:

A

相似文献/References:

[1]张丽平,展锐,丁玉芬.肤宁洁乳膏对淹渍皮肤保护作用的临床研究[J].西部中医药,2012,25(05):64.
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备注/Memo

备注/Memo:
收稿日期：2017-12-20 *基金项目：湖北省卫生计生科研基金项目(编号 WJ2017F080)；十堰市科技局项目(编号16Y68)。 作者简介：李志浩(1979—)，男，硕士学位，主管中药师。研究方向：药物分析。 △通讯作者：姜雪强(1979—)，男，博士学位，副主任医师。研究方向：肝病的防治。
更新日期/Last Update: 2019-03-15