[1]王奇钰,吴晗,唐立垚,等.黄芪多糖对VEGF-C表达的抑制与结直肠癌细胞内淋巴管生成的相关性研究[J].西部中医药,2023,36(03):47-51.[doi:10.12174/j.issn.2096-9600.2023.03.09]
　WANG Qiyu,WU Han,TANG Liyao,et al.Correlation Study on the Inhibition of VEGF-C Expression by Astragalus Polysaccharides and Lymphangiogenesis in Colorectal Cancer Cells[J].Western Journal of Traditional Chinese Medicine,2023,36(03):47-51.[doi:10.12174/j.issn.2096-9600.2023.03.09]
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黄芪多糖对VEGF-C表达的抑制与结直肠癌细胞内淋巴管生成的相关性研究
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
36
期数:
2023年03期
页码:
47-51
栏目:
出版日期:
2023-03-14

文章信息/Info

Title:
Correlation Study on the Inhibition of VEGF-C Expression by Astragalus Polysaccharides and Lymphangiogenesis in Colorectal Cancer Cells
作者:
王奇钰1,2,3, 吴晗1,3, 唐立垚1, 王雅楠1,3, 高峰1
1.解放军联勤保障部队第940医院，甘肃 兰州 730050
2.甘肃省干细胞与基因药物重点实验室，甘肃 兰州 730050
3.甘肃中医药大学，甘肃 兰州 730000
Author(s):
WANG Qiyu1,2,3, WU Han1,3, TANG Liyao1, WANG Ya'nan1,3, GAO Feng1
1.Hospital 940 of PLA Joint Logistics Support Force, Lanzhou 730050, China
2.Gansu Provincial Key Lab for Stem Cells and Gene Drugs, Lanzhou 730050, China
3.Gansu University of Chinese Medicine, Lanzhou 730000, China
关键词:
结直肠癌; 淋巴结转移; VEGF-C/LYVE-1; 黄芪多糖
Keywords:
colorectal cancer; lymph node metastasis; VEGF-C/LYVE-1; astragalus polysaccharides
分类号:
R273
DOI:
10.12174/j.issn.2096-9600.2023.03.09
文献标志码:
A
摘要:
目的了解黄芪多糖（astragalus polysaccharides，APS）作用后的人结直肠癌（colorectal cancer，CRC）细胞中血管内皮生长因子C（vascular endothelial growth factor-C，VEGF-C）的表达，探讨其对CRC淋巴结转移的影响及可能机制。 方法对不同浓度APS处理后的LoVo细胞进行划痕实验，并应用Western印迹法和RT-PCR法分析VEGF-C/LYVE-1的表达。 结果与对照组相比，APS作用后的LoVo细胞中VEGF-C和LYVE-1的蛋白表达降低，且随浓度梯度降低（P＜0.05）。与对照组相比，APS作用后的LoVo细胞中VEGF-C和LYVE-1基因的相对荧光表达量随浓度梯度降低，差异均有统计学意义（P＜0.05）。 结论APS可能参与肿瘤淋巴管的生成，在CRC淋巴转移中发挥重要作用，且APS可能是一种潜在的肿瘤淋巴管生成的抗淋巴管生成剂，可用于预防和治疗CRC淋巴转移。
Abstract:
ObjectiveTo survey the expressions of VEGF-C in human colorectal cancer cells after the actions of APS, and explore its effects on lymph node metastasis in colorectal cancer and the possible mechanism. MethodsScratch experiment was performed on LoVo cells treated with different concentrations of APS, Western blot and RT-PCR method were applied to analyze the expressions of VEGF-C/LYVE-1. ResultsCompared with the control group, the expressions of VEGF-C and LYVE-1 protein reduced in LoVo cells after the actions of APS, and the protein relative expressions lowered when APS concentration reduced (P<0.05). Compared with the control group, the relative fluorescence expressions of VEGF-C and LYVE-1 genes in LoVo cells lowered after the actions of APS with the concentration gradient, and the difference had statistical meaning (P<0.05). ConclusionAPS might be involved in tumor lymphangiogenesis and play an important role in lymph node metastasis, and APS might be a potential anti-lymphangiogenic agent of tumor lymphangiogenesis, which could be used to prevent and treat lymphatic metastasis of colorectal cancer.

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备注/Memo

备注/Memo:
王奇钰（1995—），男，硕士学位，医师。研究方向：结直肠癌的中医药防治。军后综〔2016〕号面上项目（16BJZ48）。
更新日期/Last Update: 2023-03-15