[1]王春爱,谢建琴,王迎斌,等.豆腐果苷对疼痛大鼠脊髓背角c—fos表达的影响[J].西部中医药,2012,25(12):10-12.
　WANG Chun-ai,XIE Jian-qin,WANG Ying-bin,et al.Effect of Helicid on C-fos Expressions of Cornu Dorsale Medullae Spinalis in Rats Suffering from Pain[J].Western Journal of Traditional Chinese Medicine,2012,25(12):10-12.
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豆腐果苷对疼痛大鼠脊髓背角c—fos表达的影响
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
25
期数:
2012年12期
页码:
10-12
栏目:
出版日期:
2012-12-15

文章信息/Info

Title:
Effect of Helicid on C-fos Expressions of Cornu Dorsale Medullae Spinalis in Rats Suffering from Pain
文章编号:
1004-6852(2012)12-0010-03
作者:
王春爱1，谢建琴1，王迎斌2，贾国龙2
1 甘肃省中医院，甘肃 兰州 730050；2 兰州大学第二医院
Author(s):
WANG Chun-ai1, XIE Jian-qin1, WANG Ying-bin2, JIA Guo-long2
1 Gansu Province Hospital of Traditional Chinese Medicine, Lanzhou 7300501, China; 2 The Second Hospital of Lanzhou University
关键词:
豆腐果苷; 痛阈; c-fos; 免疫组化
Keywords:
helicid;  pain threshold;  c-fos;  immunohistochemical method
分类号:
R285.5
文献标志码:
A
摘要:
目的：观察豆腐果苷(helicid)对坐骨神经慢性压迫伤(CCI)后大鼠的镇痛作用及脊髓c-fos表达的影响.方法：32只Wistar雄性大鼠随机分为4组(n=8)；分别为Sham组、CCI组、H1组和H2组。4组分别于CCI手术前1天、手术后第9天进行实验，测后肢的机械痛阈值（MWT）。H1组和H2组手术后10天开始给药，测定1、3、5、7、10天后肢MWT,第14天取大鼠脊髓腰膨大部位免疫组化染色检测Fos免疫阳性细胞。结果：豆腐果苷可明显提高CCI模型大鼠的疼痛阈值(P＜0.05),抑制由疼痛所引起的脊髓背角c-fos表达增强的效应(P＜0.05)。结论：口服豆腐果苷能剂量依赖性提高CCI大鼠疼痛阈值,降低机械痛觉过敏,抑制神经损伤后脊髓背角c-fos的表达。
Abstract:
Objective: To verify the analgesic effects of helicid on chronic constriction injury of the sciatic nerve (CCI) and c-fos expressions of rats. Method: Thirty-two rats were randomly divided into four groups (n=8):sham group, CCI group, H1 group and H2 group. Four groups were experimented on at the 1st day before CCI surgery and the ninth day after the surgery to detect MWT of hind legs. H1 and H2 groups were given with medicine at the tenth day after the surgery to determine MWT of hind legs at the 1st, 3rd, 5th, 7th and 10th day, at the 14th day lumbar and spinal enlargements were took and fos immunoreactive positive cells were detected with immunohistochemical staining method. Result: Helicid can effectively raise pain threshold of CCI model rats (P＜0.05), and inhibit c-fos expressions raise of cornu dorsale medullae spinalis induced by pain. Conclusion: Oral helicid can raise pain threshold of CCI rats dose-dependently, decrease mechanical pain allergy and inhibit c-fos expressions of cornu dorsale medullae spinalis after neural injury.

参考文献/References:

［1］ 梁诗颸，邓少婷.豆腐果苷片抗炎镇痛作用研究［J］.宜春学院学报：自然科学版，2006，28(2)：104-105. ［2］ Bennett GJ，Xie YK.A peripheral mononeuropathy in rat that produces disorders of pain sensation like those seen in man［J］.Pain，1988，33(1)：87-107. ［3］ Zimmermann M.Pathobiology of neuropathic pain［J］.Eur J Pharmacol，2001，429(1-3)：23-37. ［4］ Max MB.Clarifying the definition of neuropathic pain［J］.Pain，2002，96(3)：406-407. ［5］ 杭黎华，戴体俊，曾因明.NMDA 受体与吸入麻醉药的镇痛作用的关系［J］.国外医学：麻醉学与复苏分册，2005，26(2)：108-111. ［6］ 项旭映.神经病理性疼痛的机制研究进展［J］.亚太传统医学，2007，3(9)：80-82. ［7］ 钟敏，胡兴国，曾因明，等.大鼠切口疼痛对脊髓背角Fos蛋白的影响［J］.中国疼痛医学杂志，2002，8(3)：178-179. ［8］ 金小高，罗爱林，张广雄.三种大鼠神经病理性疼痛模型的制备和效果比较［J］.临床麻醉学杂志，2005，21(5)：338-340.

相似文献/References:

[1]王春爱,王迎斌,贾国龙,等.豆腐果苷对大鼠坐骨神经压迫性模型机械痛敏和热痛敏的影响[J].西部中医药,2012,25(07):20.
　WANG Chun-ai,WANG Ying-Bin,JIA Guo-long,et al.The Effect of Helicid on MWT and TWL in Rat Model with Chronic Compression Injury of Sciatic Nerve[J].Western Journal of Traditional Chinese Medicine,2012,25(12):20.

备注/Memo

备注/Memo:
收稿日期：2012-09-20 * 基金项目：甘肃省自然科学基金项目(编号 1010RJZA129) 作者简介：王春爱(1971—)，女，硕士学位，副主任医师。研究方向：疼痛、重症医学。
更新日期/Last Update: 1900-01-01