[1]舒畅,杨丽霞,程涛,等.基于 Smads 信号通路探讨止消通脉宁干预 TGF-β1诱导 HK-2 细胞转分化的研究*[J].西部中医药,2013,26(06):10-12.
　SHU Chang,YANG Lixia,CHENG Tao,et al.Research on Intervention of ZhiXiao TongMaiNing to Trans-differentiation of Human Renal Tubular Epithelial HK-2 Cell Induced by TGF-β1 Based on Smads Signaling Pathway[J].Western Journal of Traditional Chinese Medicine,2013,26(06):10-12.
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基于 Smads 信号通路探讨止消通脉宁干预 TGF-β1诱导 HK-2 细胞转分化的研究*
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
26
期数:
2013年06期
页码:
10-12
栏目:
出版日期:
2013-06-15

文章信息/Info

Title:
Research on Intervention of ZhiXiao TongMaiNing to Trans-differentiation of Human Renal Tubular Epithelial HK-2 Cell Induced by TGF-β1 Based on Smads Signaling Pathway
文章编号:
1004-6852(2013)06-0010-03
作者:
舒畅1，杨丽霞2，程涛2，刘铜华3△，吴丽丽3，Margetts，Peter Joseph4
1 甘肃中医学院，甘肃 兰州 730000；2 甘肃省中医药研究院；3 北京中医药大学；4 McMaster University(麦克马斯特大学)
Author(s):
SHU Chang1,YANG Lixia2, CHENG Tao2, LIU Tonghua3△, W U Lili3, Margetts Peter Joseph4
1 Gansu University of Traditional Chinese Medicine, Lanzhou 730000, China;2 Gansu Province A cademy of Chinese Medicine; 3 Beijing University of Chinese Medicine；4 McMaster University
关键词:
止消通脉宁; TGF-β1; HK-2 细胞; TβRI; TβRII; Smad 2; Smad 3
Keywords:
ZhiXiao TongMaiNing (ZXTMN);  transforming growth factor-β1(TGF-β1);  HK-2 cells; TβRI;  TβRII;  Smad 2;  Smad 3
分类号:
R285.5
文献标志码:
A
摘要:
目的：探讨止消通脉宁含药血清对转化生长因子 -β1(TGF-β1)诱导的人肾小管上皮细胞(HK-2)转分化 Smad 信号通路的影响。方法：将 HK-2 细胞用含 10%胎牛血清的 DMEM/F12(1∶1)培养基培养；实验分为6组：空白对照组、单纯 TGF-β1诱导组(TGF-β110 ng/mL)、空白血清对照组(TGF-β110 ng/mL +10%空白血清)、中药含药血清低剂量组(TGF-β110 ng/mL +10%低剂量止消通脉宁含药血清)、中药含药血清中 剂量组 (TGF-β110 ng/mL+10%中剂量止消通脉宁含药血清)、中药含药血清高剂量组(TGF-β110 ng/mL +10%高剂量止消通脉宁含药血清)。药物干预 24 小时后，荧光定量 PCR 检测 TβRI、TβRⅡ的 mRNA 表达，Western blot 检测 Smad 2、Smad 3 的蛋白表达。结果：HK-2 细胞经 TGF-β1诱导后，TβRI、TβRⅡ 的 mRNA 表达和 Smad 2、Smad 3 的蛋白表达显著上升，与空白对照组相比差异有统计学意义(P＜0.05)，经止消通脉宁含药血清干预后，其表达逐步下降，与单纯 TGF-β1诱导组相比差异有统计学意义(P＜0.05)。而空白血清无此作用。结论：止消通脉宁能够调控TGF-β1诱导的人肾小管上皮细胞转分化 Smad 信号通路，在一定程度上具有抑制肾间质纤维化的作用。
Abstract:
Objective: To explore the effects of ZhiXiao TongMaiNing (ZXTMN) on the Smads signaling pathway of human renal tubular epithelial cells HK-2 which was induced by transforming growth factor-β1(TGF-β1).Method: The HK-2 cells were cultured with DMEM /F12 (1:1) contained 10% fetal bovine serum. There were six groups: the control group, TGF-β1group (TGF-β110 ng/mL), blank control group of serum(TGF-β110 ng/mL+10%blank sencm) low dosage group of serum (TGF-β110 ng/mL +rat serum containing 10% low dosage of ZXTM),moderate dosage group of serum (TGF-β110 ng/mL + rat serum containing 10% moderate dosage of ZXTM) and high dosage group of serum(TGF-β110 ng/mL + rat serum containing 10% high dosage of ZXTM). After intervening for 24hours, mRNA expressions of TβRI and TβRII were tested with fluorescence quantitative PCR assay and the expressions of Smad 2 and Smad 3 protein by Western blot assay. Result: The mRNA expressions of TβRI and TβRII and the expressions of Smad 2 and Smad 3 protein were remarkably raised when HK-2 cells were induced by TGF-β1, it showed statistical meaning compared with blank control group (P＜0.05), but the expressions were decreased significantly after intervening with ZXTMN, it had statistical meaning compared with TGF-β1group (P＜0.05).Only rat serum had no such effect. Conclusion: ZXTMN could regulate the smads signaling pathway of human renal tubular epithelial cells HK-2 induced by TGF-β1, and inhibit renal fibrosis to a certain extent.

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备注/Memo

备注/Memo:
收稿日期：2012-12-17 * 基金项目：科技国际科技合作项 目 (编号2009DFA31520)，北京中医药大学创新团队项目(编号2011-CXTD-19) 作者简介：舒畅(1978—)，女，硕士学位，讲师。研究方向：糖尿病的中医诊治。 △通讯作者：刘铜华（1963—），男，博士研究生导师，博士学位，教授。研究方向：糖尿病及其并发症的中医药防治。
更新日期/Last Update: 2013-06-15