[1]狄建新,王杰.螺旋藻多糖对辐射致肝癌细胞的细胞增殖及凋亡率的影响[J].西部中医药,2013,26(07):20-22.
　DI Jianxin,WANG Jie.Experimental Study of Spriulina Polysacchrides on the Proliferation and Apoptosis Rate of Hepatoma Carcinoma Cells Induced by Radiation[J].Western Journal of Traditional Chinese Medicine,2013,26(07):20-22.
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螺旋藻多糖对辐射致肝癌细胞的细胞增殖及凋亡率的影响
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
26
期数:
2013年07期
页码:
20-22
栏目:
出版日期:
2013-07-15

文章信息/Info

Title:
Experimental Study of Spriulina Polysacchrides on the Proliferation and Apoptosis Rate of Hepatoma Carcinoma Cells Induced by Radiation
文章编号:
1004-6852(2013)07-0020-03
作者:
狄建新1，王杰2
1 民勤县人民医院，甘肃 民勤 733300； 2 兰州大学基础医学院
Author(s):
DI Jianxin1, WANG Jie2
1 Minqin County People′s Hospital, Minqin 733300, China; 2 Basic Medical School of Lanzhou University
关键词:
螺旋藻多糖; 肝癌细胞; 增殖抑制; 凋亡率
Keywords:
spriulina polysacchrides;  hepatoma carcinoma cells;  proliferation inhibition;  apoptosis rate
分类号:
R735.7
文献标志码:
A
摘要:
目的:评价螺旋藻多糖对人肝癌细胞株HepG2增殖的抑制作用及辐射增敏作用。方法：用MTT法检测不同时间-浓度条件下，螺旋藻多糖作用后的人肝癌细胞株HepG2细胞存活分数，计算细胞增殖抑制率；用流式细胞术检测不同时间-剂量条件下，螺旋藻多糖对X线辐射后人肝癌细胞株HepG2周期阻滞及凋亡率的影响。结果：螺旋藻多糖对人肝癌细胞株HepG2具有体外增殖抑制作用。螺旋藻多糖可将肝癌细胞阻滞于G0/G1和S期并诱导其凋亡，流式细胞仪检测有凋亡峰出现。结论：螺旋藻多糖对肝癌细胞HepG2体外增殖抑制作用表现为时间-浓度依赖性效应。一定条件下螺旋藻多糖对人肝癌细胞HepG2具有辐射增敏作用。
Abstract:
Objective: To evaluate the inhibition of spriulina polysacchrides to the proliferation of HepG2 human liver cancer cell line and explore its radiation sensibilization. Method: Survival fractions of HepG2 human liver cancer cell line were tested with MTT method under different times and concentrations when the cell lines were dealt with spriulina polysacchrides to calculate inhibition ratio of cellular proliferation; Effects of spriulina polysacchrides on apoptosis rate and cell cycle arrest of HepG2 human liver cancer cell line which was radiated with X-ray were detected under different times and dosages with flow cytometry. Result: Spriulina polysacchrides could inhibit the proliferation of HepG2 human liver cancer cell line in vitro. Spriulina polysacchrides could arrest liver cancer cells at G0/G1 and S phases, the detection of flow cytometry showed that there was apoptotic peak appeared. Conclusion: The effects of spriulina polysacchrides on the inhibition of HepG2 human liver cancer cell extracorporeal proliferation are time-concentration dependent relationship. Under a certain condition, spriulina polysaccharides could enhance radiation sensibilization of HepG2 human liver cancer cell.

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[1]何敏,岳鹏莹,白宝平.苦参碱通过Pink1/Parkin途径抑制线粒体途径促进肝癌细胞凋亡的机制?[J].西部中医药,2021,34(11):50.[doi:10.12174/j.issn.2096-9600.2021.11.11]
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备注/Memo

备注/Memo:
收稿日期：2013-03-12 作者简介：狄建新(1965—)，男，副主任医师。研究方向：肝脏疾病的中西医结合诊治。
更新日期/Last Update: 2013-07-15