[1]郝建明,郭文宾,刘兰生,等.十三味马钱子丸中士的宁和马钱子碱的HPLC测定[J].西部中医药,2014,27(06):20-23.
　HAO Jianming,GUO Wenbin,LIU Lansheng,et al.Determination of Strychnine and Brucine in ShiSanWei MaQianZi Pill by HPLC[J].Western Journal of Traditional Chinese Medicine,2014,27(06):20-23.
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十三味马钱子丸中士的宁和马钱子碱的HPLC测定
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
27
期数:
2014年06期
页码:
20-23
栏目:
出版日期:
2014-06-15

文章信息/Info

Title:
Determination of Strychnine and Brucine in ShiSanWei MaQianZi Pill by HPLC
文章编号:
1004-6852(2014)06-0020-04
作者:
郝建明1，郭文宾2，刘兰生3，范潇予4
1 酒泉市中医院，甘肃 酒泉 735000； 2 甘肃河西制药有限责任公司； 3 甘肃省食品药品检验所； 4 沈阳药科大学
Author(s):
HAO Jianming1, GUO Wenbin2, LIU Lansheng3, FAN Xiaoyu4
1 Jiuquan Municipality TCM Hospital, Jiuquan 735000, China; 2 Gansu Hexi Pharmaceutical Company Ltd.; 3 Gansu Provincial Institute for Food and Drug Control; 4 Shenyang Pharmaceutical University
关键词:
HPLC; 十三味马钱子丸; 士的宁; 马钱子碱
Keywords:
HPLC;  ShiSanWei MaQianZi pill;  strychnine;  brucine
分类号:
R284.1
摘要:
目的：建立一种同时测定十三味马钱子丸中士的宁和马钱子碱的高效液相色谱法。方法：采用高效液相法在同一色谱条件，Dikma C18（4.6 mm×250 mm，5 μm）色谱柱；流动相为乙腈-0.01 mol/L庚烷磺酸钠与0.02 mol/L磷酸二氢钾等量混合溶液（用10%磷酸调节pH值2.8）（10：90）；检测波长为260 nm；柱温为30℃；流速为1.0 mL/min；对十三味马钱子丸中两种成分同时进行测定。结果：士的宁、马钱子碱分别在0.262～1.310 μg（r=0.999 8）、0.172～0.860μg （r=0.999 5）线性关系良好。两者的平均回收率分别为97.96%（RSD为1.95%）、98.06%（RSD为2.01%）。结论：所建立的方法简便、专属、重复性好，可用于十三味马钱子丸的质量控制。
Abstract:
Objective: To establish a method for determinate strychnine and brucine in ShiSanWei MaQianZi pill simultaneously by HPLC. Methods: HPLC was adopted under the same condition, Dikma C18(4.6 mm×250 mm, 5 μm) chromatographic column; mobile phase: acetonitrile-0.01mol/L sodium heptanesulfonate and 0.02mol/L potassium dihydrogen phosphate mix solution (regulating pH value 2.8 with 10% phosphoric acid) (10: 90); detection wavelength was 260nm; column temperature: 30℃; flow rate was 1.0mL/min; two kinds of ingredients in ShiSanWei MaQianZi pill were determined simultaneously. Results: Strychnine and brucine showed better linear relationship in the ranges between 0.262 and 1.310μg (r=0.999 8), 0.172 and 0.860 μg (r=0.999 5). Average recovery rates of both were 97.96% (RSD=1.95%), and 98.06% (RSD=2.01%) respectively. Conclusion: The method, simple, exclusive and good repeatability, could be used for quality control of ShiSanWei MaQianZi pill.

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备注/Memo

备注/Memo:
收稿日期：2013-11-20作者简介：郝建明(1968—)，男，主管中药师。研究方向：医院制剂质量标准与工艺及临床药学。
更新日期/Last Update: 2014-06-15