[1]古秋莉,黄聪琳,姜华.镰形棘豆黄酮苷元对免疫抑制小鼠非特异免疫功能的影响[J].西部中医药,2016,29(04):14-16.
　GU Qiuli,HUANG Conglin,JIANG Hua.The Impact of Flavonoid Glycosides Extracted from LianXing JiDou on Nonspecfic Immune Function of the Mice with Immunodepression[J].Western Journal of Traditional Chinese Medicine,2016,29(04):14-16.
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镰形棘豆黄酮苷元对免疫抑制小鼠非特异免疫功能的影响()
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
29
期数:
2016年04期
页码:
14-16
栏目:
出版日期:
2016-04-15

文章信息/Info

Title:
The Impact of Flavonoid Glycosides Extracted from LianXing JiDou on Nonspecfic Immune Function of the Mice with Immunodepression
文章编号:
1004-6852(2016)04-0014-03
作者:
古秋莉1，黄聪琳1，姜华2
1 甘肃省中医院，甘肃 兰州 730050； 2 甘肃省中医药研究院
Author(s):
GU Qiuli1, HUANG Conglin1, JIANG Hua2
1 Gansu Provincial Hospital of Traditional Chinese Medicine, Lanzhou 730050, China;2 Gansu Provincial Academy of Chinese Medicine
关键词:
镰形棘豆; 黄酮苷元; 非特异性免疫; 动物实验; 小鼠; 环磷酰胺
Keywords:
LianXing JiDou;  flavonoid glycosides;  nonspecific immune function;  animal experiment;  the mice;  cyclophosphamide
分类号:
R285.5
摘要:
目的：探讨藏药镰形棘豆黄酮苷元对免疫抑制小鼠非特异性免疫功能的影响。方法：将60只昆明种小鼠随机分为6组：正常组，模型组，镰形棘豆黄酮苷元高、中、低剂量组(336、168、84 mg/kg)和盐酸左旋咪唑组 (25 mg/kg)，每组10只。镰形棘豆黄酮苷元高、中、低剂量组分别灌胃给予不同剂量的黄酮苷元，正常组和模型组灌服等体积5%吐温-80溶液，共10天。第8天开始，以注射环磷酰胺制备免疫低下小鼠模型，除正常组腹腔注射生理盐水外，其他各组分别腹腔注射环磷酰胺，共3天。采用巨噬细胞碳粒廓清实验、巨噬细胞吞噬鸡红细胞实验、免疫抑制小鼠器官指数的测定方法，观察镰形棘豆黄酮苷元对免疫抑制小鼠非特异性免疫功能的影响。结果：与模型组比较，镰形棘豆黄酮苷元各剂量组能有效提高免疫抑制小鼠的吞噬百分率、廓清指数(K)和吞噬系数(α)，差异有统计学意义(P＜0.05)，增加免疫器官脾脏和胸腺的器官指数，差异有统计学意义(P＜0.05)。结论：镰形棘豆黄酮苷元能够增强环磷酰胺所致免疫功能抑制小鼠非特异性免疫功能。
Abstract:
Objective: To explore the influence of flavonoid glycosides extracted from Tibetan medicine LianXing JiDou(Oxytropis falcata Bunge) on nonspecific immune function of the mice with immunodepression. Methods: Sixty mice were randomized into six groups: the normal group, the control group, high, moderate and low doses groups of flavonoid glycosides extracted from LianXing JiDou(336, 168 and 84 mg/kg) and levamisole hydrochloride group (25 mg/kg), ten mice each group. High, moderate and low doses groups of flavonoid glycosides extracted from LianXing JiDou began to accept intragastric administration of flavonoid glycosides in different doses respectively, the mice in the normal group and the model group were drenched with the same amount of 5% tween-80 solutions, for ten days. The mice models with immunodepression were prepared by injecting cyclophosphamide since the eighth day, except the normal group received peritoneal injection of physiological saline, other groups accepted peritoneal injection of cyclophosphamide for three days. The influence of flavonoid glycosides extracted from LianXing JiDou on nonspecific immune function of immunodepression mice was observed by adopting macrophage carbon clearance test, macrophage phagocytosis of chicken red blood cells and detecting organ indexes of immunodepression mice. Results: Compared with the model group, different doses groups of flavonoid glycosides extracted from LianXing JiDou could effectively improve phagocytic percentage, clearance rate index(K) and Phagocytic coefficient (α) the difference had statistical meaning (P＜0.05), increase organ indexes of the spleen and thymus gland, and the difference showed statistical meaning(P＜0.05). Conclusion: Flavonoid glycosides extracted from Tibetan medicine LianXing JiDou could enhance nonspecific immune function of cyclophosphamide-induced immunodepression mice.

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[6]张丽,姜华△,黄聪琳.藏药黄酮苷元抗炎作用的初步研究*[J].西部中医药,2015,28(02):50.

备注/Memo

备注/Memo:
收稿日期：2015-11-17*基金项目：国家自然科学基金项目(编号81260633)。作者简介：古秋莉(1964—)，女，副主任药师。研究方向：医院药学。
更新日期/Last Update: 2016-04-15