[1]赵冬耕,凌昌全△.芪术方诱导人肝癌细胞SMMC-7721凋亡机制研究[J].西部中医药,2019,32(03):44-48.
　ZHAO Donggeng,LING Changquan.Study on the Mechanism of QiZhu Prescriptions Inducing the Apoptosis of Human Liver Cancer Cells SMMC-7721[J].Western Journal of Traditional Chinese Medicine,2019,32(03):44-48.
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芪术方诱导人肝癌细胞SMMC-7721凋亡机制研究
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
32
期数:
2019年03期
页码:
44-48
栏目:
出版日期:
2019-03-15

文章信息/Info

Title:
Study on the Mechanism of QiZhu Prescriptions Inducing the Apoptosis of Human Liver Cancer Cells SMMC-7721
文章编号:
1004-6852(2019)03-0044-05
作者:
赵冬耕1，2，凌昌全3△
1 上海中医药大学，上海 201203； 2 南通市中医院； 3 第二军医大学长海医院
Author(s):
ZHAO Donggeng1, 2, LING Changquan3△
1 Shanghai University of TCM, Shanghai 201203, China; 2 Nantong Hospital of Traditional Chinese Medicine; 3 Changhai Hospital Affiliated to the Second Military Medical University
关键词:
芪术方; PI3K/AKT信号通路; 凋亡; 人肝癌细胞SMMC-7721
Keywords:
QiZhu prescriptions;  PI3K/AKT signal channel;  apoptosis;  human liver cancer cells SMMC-7721
分类号:
R285.5
摘要:
目的：探讨芪术方(QZP)诱导人肝癌细胞SMMC-7721凋亡的分子机制。方法：以不同浓度的QZP干预人肝癌细胞SMMC-7721，流式细胞仪检测细胞凋亡及周期分布情况，RT-PCR法检测AKT1、AKT2、Caspase-9mRNA 的表达；蛋白质印迹法检测p-AKT、PTEN蛋白表达。结果：QZP能阻滞人肝癌细胞SMMC-7721从G0/G1期进入S期，并诱发细胞凋亡；能抑制AKT1、AKT2表达，提高Caspase-9表达，且跟浓度呈正比例关系。结论：QZP能诱导人肝癌细胞SMMC-7721凋亡和阻滞细胞周期，其机制可能为促进人肝癌细胞SMMC-7721 PTEN表达的增加，使得PI3K/AKT信号通路激活受阻，同时抑制p-AKT、AKT1、AKT2的表达，激活下游Caspase-9促凋亡分子的表达，从而为PI3K/AKT信号通路介导细胞凋亡这一设想提供了理论依据。
Abstract:
Objective: To discuss molecular mechanism of QiZhu prescription (QZP) inducing the apoptosis of human liver cancer cells SMMC-7721. Methods: Different concentrations of QZP were used to intervene human liver cancer cells SMMC-7721, flow cytometry was used to detect cellular apoptosis and cycle distribution conditions, RT-PCR was adopted to detect the expressions of AKT1, AKT2 and Caspase-9mRNA, Western blotting was used to detect the expressions of p-AKT and PTEN protein. Results: QZP could arrest human liver cancer cells SMMC-7721 transiting from G0/G1 phase to S phase, induce cellular apoptosis; it could inhibit the expressions of AKT1 and AKT2, raise the expression of caspase-9, and the effects of QZP varies directly with the concentrations. Conclusion: QZP could induce the apoptosis of human liver cancer cell SMMC-7221 and arrest cellular cycle, and it might increase the expressions of human liver cancer cells SMMC-7721 PTEN, block the activation of PI3K/AKT signal channel, inhibit the expressions of p-AKT, AKT1 and AKT2, and activate the expressions of caspase-9, therefore to provide theoretical evidence for PI3K/AKT signal channel mediating cellular apoptosis.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期：2018-02-27 *基金项目：上海市中医药事业发展三年行动计划（编 号ZY3-LCPT-2-1004）；长海医院1255学科建设计划课题（编号 CH125521200）;上海市科学技术委员会医学引导项目（编号 15401931700）。 作者简介：赵冬耕(1985—)，男，在读博士研究生，主治医师。研究方向：肿瘤的中西医结合治疗。
更新日期/Last Update: 2019-03-15