[1]张肖建,董璐萌,高晗,等.土茯苓配方颗粒水提液HPLC特征图谱研究[J].西部中医药,2021,34(01):20-23.[doi:10.12174/j.issn.2096-9600.2021.01.05]
　ZHANG Xiaojian,DONG Lumeng,GAO Han,et al.Study on Fingerprint of Water Extract of Tufuling Dispensing Granules by HPLC[J].Western Journal of Traditional Chinese Medicine,2021,34(01):20-23.[doi:10.12174/j.issn.2096-9600.2021.01.05]
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土茯苓配方颗粒水提液HPLC特征图谱研究
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
34
期数:
2021年01期
页码:
20-23
栏目:
质量分析
出版日期:
2021-01-15

文章信息/Info

Title:
Study on Fingerprint of Water Extract of Tufuling Dispensing Granules by HPLC
作者:
张肖建1, 董璐萌1, 高晗1, 李军山1,2, 李振江1
1.神威药业集团有限公司,河北 石家庄 051430
2.河北省中药配方颗粒工程技术研究中心
Author(s):
ZHANG Xiaojian1, DONG Lumeng1, GAO Han1, LI Junshan1,2, LI Zhenjiang1
1.China Shineway Pharmaceutical Group Ltd., Shijiazhuang 051430, China
2.Research Center for Hebei Provincial Herbal Dispensing Granules Engineering Technology
关键词:
土茯苓; 配方颗粒; 特征图谱; 高效液相色谱
Keywords:
dispensing granules; fingerprint; HPLC
分类号:
R284.1
DOI:
10.12174/j.issn.2096-9600.2021.01.05
摘要:
目的建立土茯苓配方颗粒水提液高效液相色谱特征图谱，为土茯苓配方颗粒的质量控制提供参考。 方法采用kromasil C18色谱柱（4.6 mm×250 mm，5 μm），流动相：甲醇-0.05%磷酸水溶液，系统梯度洗脱，流速：1.0 mL/min，柱温：30℃，检测波长：280 nm。 结果对10批土茯苓配方颗粒进行测定，确定了土茯苓配方颗粒指纹图谱中14个共有峰，且10批样品的相似度在0.9以上。 结论该方法准确可靠，重复性好，可用于土茯苓配方颗粒的鉴别和质量评价。
Abstract:
ObjectiveTo provide the reference for quality control of Tufuling (Smilacis Glabrae Rhizoma) dispensing granules by determining the fingerprint of Tufuling dispensing granules with HPLC. MethodsKromasil C18 chromatographic column (4.6 mm×250 mm, 5 μm), mobile phase: methanol-0.05% phosphoric acid aqueous solution, systematic gradient elution, flow rate: 1.0 mL/min, column temperature:30℃, detection wavelength: 280nm. ResultsAfter determining ten batches of Tufuling dispensing granules, 14 common peaks were confirmed in the fingerprint of Tufuling dispensing granules, the similarity of ten batches of samples were higher than 0.9. ConclusionThe method, accurate, reliable and reproducible, could be used for quality assessment and identification of TuFuLing dispensing granules.

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备注/Memo

备注/Memo:
张肖建（1986—），女，工程师。研究方向：配方颗粒工艺及质量标准研究。河北省科技计划项目（16272510，17272505D）。
更新日期/Last Update: 2021-01-15