[1]王倩倩,孙连庆.丹酚酸B通过下调细胞凋亡及炎性损伤减轻高糖诱导的RSC96细胞损伤[J].西部中医药,2021,34(02):22-25.[doi:10.12174/j.issn.2096-9600.2021.02.06]
　WANG Qianqian,SUN Lianqing.Salvianolic Acid B Alleviates RSC96 Cell Injury Induced by High Glucose Via Suppressing Cellular Apoptosis and Inflammatory Damage[J].Western Journal of Traditional Chinese Medicine,2021,34(02):22-25.[doi:10.12174/j.issn.2096-9600.2021.02.06]
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丹酚酸B通过下调细胞凋亡及炎性损伤减轻高糖诱导的RSC96细胞损伤
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
34
期数:
2021年02期
页码:
22-25
栏目:
出版日期:
2021-02-15

文章信息/Info

Title:
Salvianolic Acid B Alleviates RSC96 Cell Injury Induced by High Glucose Via Suppressing Cellular Apoptosis and Inflammatory Damage
作者:
王倩倩, 孙连庆
西安交通大学第一附属医院,陕西 西安 710061
Author(s):
WANG Qianqian, SUN Lianqing
The First Affiliated Hospital of Xi′an Jiaotong University, Xi′an 710061, China
关键词:
丹酚酸B; 雪旺细胞; 高浓度葡萄糖; 凋亡; 炎症
Keywords:
Sal B; Schwann cells; high concentrations of glucose; apoptosis; inflammation
分类号:
R285.5
DOI:
10.12174/j.issn.2096-9600.2021.02.06
摘要:
目的观察丹酚酸B（salvianolic acid B，Sal B）对高糖（high glucose，HG）诱导的雪旺细胞增殖的影响及其对凋亡和炎性相关基因表达的影响。 方法将体外培养的大鼠雪旺细胞（RSC96细胞）分为对照组（5.6 mmol/L葡萄糖）、HG组（125 mmol/L葡萄糖）、HG+Sal B组（125 mmol/L葡萄糖+1 μmol/L Sal B）。作用72 h后，MTT法检测细胞增殖；流式细胞仪检测ROS（reactive oxygen species，ROS）含量；Western blot 法检测凋亡及炎性因子相关蛋白Bax（BCL2-Associated X，Bax）、B细胞淋巴瘤-2基因（B-cell lymphoma-2，Bcl-2）、核因子κB P65（nuclear transcription factor kappaB-P65，NF-κB-P65）、白细胞介素1β（Interleukin-1β，IL-1β）的表达。 结果1）MTT结果显示：与对照组比较，HG组OD值明显降低（P＜0.05），而HG+Sal B组OD值明显高于HG组（P＜0.05），提示HG显著抑制雪旺细胞增殖活性，Sal B可以减轻HG导致的雪旺细胞增殖活性的降低。2）流式结果显示：与对照组相比，HG组ROS含量明显增高（P＜0.05），HG+Sal B组ROS含量明显低于HG组（P＜0.05），提示HG可显著上调ROS含量，丹酚酸B可下调HG导致的氧化应激过度激活。3）与对照组比较，HG组Bax，P65，IL-1β的蛋白表达水平明显增高，Bcl-2表达水平下调（P＜0.05）；与HG组比较，HG+Sal B组Bax，P65，IL-1β的蛋白表达降低，Bcl-2表达水平上调（P＜0.05），这提示Sal B可抑制细胞凋亡及炎性相关蛋白的表达。 结论Sal B可能通过下调细胞凋亡及炎性损伤减轻HG对RSC96细胞的损伤。
Abstract:
ObjectiveTo observe the influence of Sal B on the proliferation of HG-induced Schwann cells, and its impacts on the expressions of apoptosis and inflammation related genes. MethodsRat Schwann cells (RSC 96) cultured in vitro were allocated to the control group (5.6mmol/L glucose), HG group (125mmol/L glucose), HG+Sal B group (125mmol/L glucose+1μmol/L Sal B), After acting for 72 hours, MTT method was used to detect cellular proliferation; flow cytometry to measure the contents of ROS, Western blot method to detect the expressions of Bax, Bcl-2, NF-κB-P65 and IL-1β. Results1)MTT results showed that: compared with the control group, OD value decreased in HG group apparently (P<0.05), while OD value of HG+Sal B group was higher than that of HG group (P<0.05), which suggested that HG could notably inhibit the proliferation activity of Schwann cells, Sal B could relieve the decrease of Schwann cells proliferation activity induced by HG. 2) The results of flow cytometry displayed that: compared with the control group, ROS contents increased notably in HG group (P<0.05), ROS contents of HG+Sal B group were lower than these of HG group remarkably (P<0.05), it suggested that HG could significantly up-regulate ROS contents, Sal B could down-regulate over activation of oxidative stress induced by HG. 3) compared with the control group, the expressions of Bax, P65 and IL-1β protein lifted in HG group the expressions of Bcl-2 lowered(P<0.05); compared with HG group, the expressions of Bax, P65 and IL-1β protein reduced in HG+Sal B group the expressions of Bcl-2 lifted(P<0.05), it showed that Sal B could inhibit the expressions of cellular apoptosis and inflammation-related proteins. ConclusionSal B could alleviate the injury of RSC96 cells induced by HG which might be through down regulating cellular apoptosis and inflammatory injury.

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备注/Memo

备注/Memo:
王倩倩（1990—），女，硕士学位，住院医师。研究方向：糖尿病周围神经病变的中医药诊治。国家自然科学基金（81673785，81874447）；陕西省自然科学基础研究计划（2020JQ-515）；西安交通大学第一附属医院院级课题（2018MS-32号）。
更新日期/Last Update: 2021-02-15