[1]刘丛丽,董金菊,周双玉.紫草素对宫颈癌细胞株Caski侵袭迁移及Wnt/β-catenin信号通路的影响[J].西部中医药,2021,34(09):27-30.[doi:10.12174/j.issn.2096-9600.2021.09.08]
　LIU Congli,DONG Jinju,ZHOU Shuangyu.Effects of Shikonin on the Invasion and Migration of Cervical Cancer Cell Line Caski and Wnt/β-catenin Signaling Pathway[J].Western Journal of Traditional Chinese Medicine,2021,34(09):27-30.[doi:10.12174/j.issn.2096-9600.2021.09.08]
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紫草素对宫颈癌细胞株Caski侵袭迁移及Wnt/β-catenin信号通路的影响
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
34
期数:
2021年09期
页码:
27-30
栏目:
出版日期:
2021-09-15

文章信息/Info

Title:
Effects of Shikonin on the Invasion and Migration of Cervical Cancer Cell Line Caski and Wnt/β-catenin Signaling Pathway
作者:
刘丛丽1, 董金菊1, 周双玉2
1.湖北医药学院附属襄阳市第一人民医院西区妇产科，湖北 襄阳 441000
2.南漳县妇女儿童医院妇产科
Author(s):
LIU Congli1, DONG Jinju1, ZHOU Shuangyu2
1.Department of Obstetrics and Gynecology, Xiangyang City First People’s Hospital Affiliated to Hubei University of Medicine, Xiangyang 441000, China
2.Department of Obstetrics and Gynecology, Nanzhang Maternal and Children’s Hospital
关键词:
紫草素; Caski细胞; 侵袭; 迁移; Wnt/-Catenin信号通路
Keywords:
shikonin; Caski cells; invasion; migration; Wnt/-catenin signaling pathway
分类号:
R211
DOI:
10.12174/j.issn.2096-9600.2021.09.08
摘要:
目的探讨紫草素对宫颈癌细胞株Caski细胞侵袭迁移及Wnt/β-catenin信号通路的影响。 方法采用MTT法检测细胞增殖抑制情况；采用Transwell小室侵袭迁移实验检测Caski细胞侵袭迁移情况；采用qPCR方法检测Caski细胞GSK-3β、Wnt2B表达情况；采用Western bloting方法检测Caski细胞β-Catenin、p-β-Catenin蛋白含量。 结果紫草素可抑制Caski细胞的增殖，具有时间-浓度依赖性，并可抑制细胞的侵袭迁移，增加Caski细胞GSK-3β mRNA和p-β-Catenin蛋白的表达量，减少Caski细胞Wnt mRNA和β-Catenin蛋白的表达量。 结论紫草素可能通过抑制Caski细胞Wnt/β-Catenin信号通路抑制Caski细胞的侵袭迁移。
Abstract:
ObjectiveTo explore the effects of shikonin on the invasion and migration of cervical cancer cell line Caski and Wnt/β-catenin signaling pathway. MethodsMTT method was used to detect the inhibition of cell proliferation; Transwell chamber invasion and migration experiment was adopted to measure the invasion and migration of Caski cells; qPCR method to measure the expressions of GSK-3β and Wnt2B in Caski cells; Western bloting method to measure the contents of β-Catenin and p-β-Catenin protein in Caski cells. ResultsShikonin could inhibit the proliferation of Caski cells, in a time-concentration dependent manner, it could inhibit cellular invasion and migration, in crease the expressions of GSK-3β mRNA and p-β-Catenin protein, and lower the contents of Wnt mRNA and β-Catenin protein in Caski cells. ConclusionShikonin could restrain the invasion and migration of Caski cell possibly through inhibiting Wnt/β-catenin signaling pathway.

相似文献/References:

[1]赵阳.紫草素对荷肝癌大鼠免疫功能的影响[J].西部中医药,2018,31(09):26.
　ZHAO Yang.Effects of Shikonin on Immunologic Function of the Rats Suffering Liver Cancer[J].Western Journal of Traditional Chinese Medicine,2018,31(09):26.
[2]仇晓霞,史晓敏.基于AKT信号通路探讨紫草素对宫颈癌细胞凋亡及Cyclin-D1蛋白表达的影响[J].西部中医药,2021,34(04):1.[doi:10.12174/j.issn.2096-9600.2021.04.03]
　QIU Xiaoxia,SHI Xiaomin.Influence of Shikonin on the Expressions of Cyclin-D1 Protein and the Apoptosis of Cervical Cancer Cells Based on AKT Signaling Pathway[J].Western Journal of Traditional Chinese Medicine,2021,34(09):1.[doi:10.12174/j.issn.2096-9600.2021.04.03]

备注/Memo

备注/Memo:
刘丛丽（1982—），女，主治医师。研究方向：妇产科。
更新日期/Last Update: 2021-09-15