[1]韩燕鸿,李君,潘建科,等.基于网络药理学方法探讨丹参-牡丹皮配伍治疗膝骨性关节炎的作用机制[J].西部中医药,2026,39(01):65-73.[doi:10.12174/j.issn.2096-9600.2026.01.13]
　HAN Yanhong,LI Jun,PAN Jianke,et al.Network Pharmacology-based Discussion on the Mechanism of Danshen-Mudanpi Combination in Treating Knee Osteoarthritis[J].Western Journal of Traditional Chinese Medicine,2026,39(01):65-73.[doi:10.12174/j.issn.2096-9600.2026.01.13]
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基于网络药理学方法探讨丹参-牡丹皮配伍治疗膝骨性关节炎的作用机制()

《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
39
期数:
2026年01期
页码:
65-73
栏目:
二次研究
出版日期:
2026-01-15

文章信息/Info

Title:
Network Pharmacology-based Discussion on the Mechanism of Danshen-Mudanpi Combination in Treating Knee Osteoarthritis
作者:
韩燕鸿1, 李君1, 潘建科1, 杨伟毅1, 罗明辉1, 陈红云1, 曾令烽1, 梁桂洪1, 黄和涛1, 赵第1, 侯森荣2, 吴明3, 刘军4
1.广州中医药大学第二附属医院/广东省中医院，广东 广州 510120
2.佛山市中医院，广东 佛山 528000
3.湖北省中医院，湖北 武汉 430074
4.广东省第二中医院/广东省中医药工程技术研究院，广东 广州 510095
Author(s):
HAN Yanhong1, LI Jun1, PAN Jianke1, YANG Weiyi1, LUO Minghui1, CHEN Hongyun1, ZENG Lingfeng1, LIANG Guihong1, HUANG Hetao1, ZHAO Di1, HOU Senrong
1.Second Affiliated Hospital of Guangzhou University of Chinese Medicine/Guangdong Provincial Hospital of Chinese Medicine, Guangzhou 510120, China
2.Foshan Hospital of Chinese Medicine, Foshan 528000, China
3.Hubei Provincial Hospital of TCM, Wuhan 430074, China
4.Guangdong Second Traditional Chinese Medicine Hospital/Guangdong Research Institute of Traditional Chinese Medicine Manufacturing Techonology, Guangzhou 510095, China
关键词:
膝骨性关节炎; 丹参; 牡丹皮; MAPK信号通路; 网络药理学
Keywords:
knee osteoarthritis; MAPK signaling pathway; network pharmacology
分类号:
R274.9
DOI:
10.12174/j.issn.2096-9600.2026.01.13
文献标志码:
A
摘要:
目的基于网络药理学方法探讨丹参-牡丹皮配伍治疗膝骨性关节炎（knee osteoarthritis，KOA）的作用机制。 方法在中药系统药理学数据库与分析平台（traditional Chinese medicine systems pharmacology database and analysis platform，TCMSP）检索并筛选丹参-牡丹皮活性成分及相关靶点蛋白，利用UniPort数据库进行靶点蛋白名转化。构建丹参-牡丹皮活性成分-靶点网络。在人类基因数据库（the human gene database，GeneCards）、Disgenet数据库、在线人类孟德尔遗传数据库（online mendelian inheritance in man，OMIM）、药物靶标数据库（therapeutic targetdatabase，TTD）、DrugBank数据库检索KOA疾病相关靶点。筛选丹参-牡丹皮作用于KOA的活性成分靶点。利用Cytoscape 3.7.2软件及其插件Bisogenet、CytoNCA对靶点进行交集分析并构建蛋白-蛋白互作（protein-protein interactions，PPI）网络图。将直接作用靶点输入核心靶基因功能注释数据库（the database for annotation visualization and integrated discovery，DAVID）进行基因本体论（gene ontology，GO）功能和京都基因与基因组百科全书（kyoto encyclopedia of genes and genomes，KEGG）通路富集分析。 结果筛选出丹参65个活性成分，牡丹皮11个活性成分。获得丹参靶点蛋白932个，牡丹皮靶点蛋白237个。通过UniPort数据库转化最终获得作用靶点214个。筛选出KOA疾病靶点1207个，得到药物与疾病共同作用靶点97个。IL-6、VEGF、TP53、ESR1、MMP、MYC、CXCL8、FOS、JUN、TNF等可能是丹参-牡丹皮治疗KOA的关键靶点；GO功能富集分析获得349个富集结果，其中包括分子功能、细胞组分、生物过程；KEGG通路富集分析获得90个相关通路。 结论丹参-牡丹皮的有效成分包括槲皮素、木樨草素等，它们通过作用于IL-6、VEGFA、TNF、MMP等靶点，主要介导MAPK信号通路，从而调控软骨细胞的凋亡与退变，减少细胞外基质的降解，抑制骨吸收，介导炎症反应，促进血管新生，加速炎症吸收和代谢分解，最终减缓软骨组织的病损进展，发挥抗KOA的治疗作用。
Abstract:
ObjectiveTo survey the mechanism of Danshen-Mudanpi combination in the treatment of knee osteoarthritis (KOA) based on network pharmacology. MethodsActive ingredients and the related target protein of Danshen-Mudanpi were searched and screened from TCMSP, the transformation of target protein names was conducted using UniPort database. Danshen-active ingredients of Danpi-target network was constructed. KOA-related targets were retrieved from GeneCards, Disgenet database, OMIM, TTD and DrugBank database. The active ingredients and targets of Danshen-Mudanpi acting on KOA were searched. Cytoscape 3.7.2 software and its plug-in Bisogenet, CytoNCA were utilized to analyze the intersection of the targets and to construct PPI network diagram. GO and KEGG pathway enrichment analysis were carried out after importing direct acting targets into DAVID. ResultsThe study identified 65 active ingredients from Danshen and 11 active ingredients from Mudanpi, with their corresponding target proteins numbering 932 and 237, respectively. Consequently, 214 targets were gained via UniPort database. A total of 1207 KOA targets were screened and 97 shared targets between medicine and diseases were gained. IL-6, VEGF, TP53, ESR1, MMP, MYC, CXCL8, FOS, JUN and TNF might be the key targets of Danshen-Mudanpi combination in the treatment of KOA; GO functional enrichment analysis gained 349 enrichment results, including molecular results, cellular component and biological process; KEGG pathway enrichment analysis uncovered 90 related pathways. ConclusionActive components of the Danshen-Mudanpi herb pair, such as quercetin and luteolin, primarily exert their effects by mediating the MAPK signaling pathway. Their action on targets like IL-6, VEGFA, TNF, and MMP leads to the regulation of chondrocyte apoptosis and degeneration, reduction of extracellular matrix degradation, inhibition of bone resorption, modulation of inflammatory responses, and promotion of angiogenesis. Consequently, this accelerates the resolution of inflamma-tion and metabolic clearance, ultimately slowing the progression of cartilage lesions and delivering therapeutic effects against KOA.

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备注/Memo

备注/Memo:
国家自然科学基金（81473698、81273781）；广东省科技计划项目（2020A1414050050）；广东省财政厅项目（〔2014〕157号、〔2018〕8号）；广东省中医药管理局项目（20164020）；广东省中医院中医药科学技术研究专项（YN2019ML08）；广东省医学科学技术研究基金项目（B2019091）。韩燕鸿（1993—），男，硕士学位，主治医师。研究方向：中医药治疗骨与关节退变及损伤的临床和循证医学研究。
更新日期/Last Update: 2026-01-15