[1]吴志成,张泽国,徐福菊,等.当归配方颗粒质量分析研究*[J].西部中医药,2014,27(04):13-17.
 WU Zhicheng,ZHANG Zeguo,XU Fuju,et al.Study on the Quality of DangGui Granules[J].Western Journal of Traditional Chinese Medicine,2014,27(04):13-17.
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当归配方颗粒质量分析研究*
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
27
期数:
2014年04期
页码:
13-17
栏目:
方药·质量分析
出版日期:
2014-04-15

文章信息/Info

Title:
Study on the Quality of DangGui Granules
文章编号:
1004-6852(2014)04-0013-05
作者:
吴志成12张泽国3徐福菊3李越峰3△
1 甘肃中医学院附属医院,甘肃 兰州 730000; 2 甘肃省中药炮制及质控工程技术研究中心; 3 甘肃中医学院
Author(s):
WU Zhicheng12 ZHANG Zeguo3 XU Fuju3 LI Yuefeng3△
1 University Hospital of Gansu Traditional Chinese Medicine, Lanzhou 730000, China; 2 The Center for TCM Preparation and Quality Control Engineering; 3 Gansu University of Traditional Chinese Medicine
关键词:
当归配方颗粒阿魏酸高效液相色谱法
Keywords:
Dangui prescription granules ferulic acid HPLC
分类号:
R284
文献标志码:
A
摘要:
目的:测定当归配方颗粒中阿魏酸含量、水分和浸出物含量,为科学评价及有效控制其质量提供可靠方法。同时,进行当归配方颗粒及当归饮片的质量比较。方法:提取方法、色谱方法均在2010版《中国药典》基础上进行了优化。高效液相色谱法,色谱柱:Kromasil C18色谱柱(4.6 mm×250 mm,5 μm),流速:1.0 mL/min,检测波长:316 nm,柱温:28℃, 流动相:乙腈-0.05%磷酸水梯度洗脱系统。结果:阿魏酸在0.033~0.165 μg(r=0.999 8,n=5)之间有良好的线性关系,平均加样回收率为98.78%,RSD=0.99%。结论:当归配方颗粒在各项含量指标上都符合当归的质量要求,阿魏酸含量高于当归饮片。采用HPLC方法测定当归配方颗粒中阿魏酸的含量,操作简单,可靠,重现性好,可作为当归配方颗粒质量控制的有效方法。
Abstract:
Objective: To provide reliable methods for scientific evaluation and effective control of the quality of Dangui (angelic sinensis) granules by determining the contents of ferulic acid, moisture and the contents of extracts in the granules. Meanwhile, to compare the quality of Dangui granules and its prepared slices. Methods: The extracting method and chromatographic process were different from the methods in pharmacopeia of 2010 edition, which were the optimization of the methods in pharmacopeia. HPLC was adopted, chromatographic column: Kromasil C18 (4.6 mm×250 mm, 5 μm), flow rate: 1.0 mL/min, detection wavelength:316 nm, the temperature:28℃, mobile phase: acetonitrile-0.05% phosphoric acid in water gradient elution system. Results: Ferulic acid showed better linear relationship in the range between 0.033 and 0.165 μg(r=0.999 8, n=5). Average recovery rate was 98.78%, RSD=0.99%. Conclusion: Dangui granules could meet quality requirement of Dangui in the contents of different indexes, the contents of ferulic acid are higher than these in prepared slices of Dangui. Meanwhile, according to the results, it is simple, reliable and reproducible to determine the contents of ferulic acid in Dangui granules by HPLC, which is an effective method for quality control of Dangui granules.

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备注/Memo

备注/Memo:
收稿日期:2013-09-09*基金项目:国家自然科学基金(编号81073077),教育部科学技术研究重点项目(编号212186),甘肃省财政厅高校基本科研业务费专项资金(编号2013-2),2010年度甘肃省自然科学基金 (编号1010RJZA212),2010甘肃中医学院引进人才科研启动基金(编号2010-3)作者简介:吴志成(1973—),男,副主任中药师。研究方向:中药材加工炮制工艺及机理。△通讯作者:李越峰(1976—),女,博士学位,硕士研究生导师,副教授。研究方向:中药材加工炮制工艺及机理。
更新日期/Last Update: 2014-04-15