[1]韦瑀龙,蓝晓庆,黄敏,等.复方感冒颗粒特征图谱与黄芩苷含量测定方法研究[J].西部中医药,2017,30(03):27-31.
 WEI Yulong,LAN Xiaoqing,HUANG Min,et al.Study on Specific Chromatogram of Compound Cold Granules and the Assay Method of Baicalin Content[J].Western Journal of Traditional Chinese Medicine,2017,30(03):27-31.
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复方感冒颗粒特征图谱与黄芩苷含量测定方法研究()
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《西部中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
30
期数:
2017年03期
页码:
27-31
栏目:
方药·质量分析
出版日期:
2017-03-15

文章信息/Info

Title:
Study on Specific Chromatogram of Compound Cold Granules and the Assay Method of Baicalin Content
文章编号:
1004-6852(2017)03-0027-05
作者:
韦瑀龙1蓝晓庆2黄敏1梁国成1
1 广西中医药大学附属瑞康医院,广西 南宁530001; 2 广西佳业科技有限公司
Author(s):
WEI Yulong1 LAN Xiaoqing2 HUANG Min1 LIANG Guocheng1
1 Ruikang Hospital Affiliated to Guangxi University of Traditional Chinese Medicine, Nanning 530001, China; 2 Guangxi Jiaye Technology Limited Company
关键词:
复方感冒颗粒特征图谱黄芩苷含量测定
Keywords:
compound cold granules specific chromatogram baicalin content determination
分类号:
R259
文献标志码:
A
摘要:
目的:建立复方感冒颗粒的特征图谱与黄芩苷的含量测定方法。方法:利用HPLC法测定复方感冒颗粒的特征图谱,采用Agilent TC C18(4.6 mm×250 mm,5 μm)色谱柱,以乙腈(A)-0.2%磷酸(B)溶液为流动相,梯度洗脱(0分钟,10%A;10分钟,14%A;25分钟,18%A;45分钟,25%A;55分钟,28%A;65分钟,42%A),流速1.0 mL/min,检测波长:266 nm,柱温:25℃。采用HPLC使用ODS-2HYPERSIL(4.6 mm×250 mm,5 μm)色谱柱,以甲醇-0.1%磷酸水(43∶57)为流动相,检测波长280 nm,流速1.0 mL/min,测定黄芩苷的含量。结果:建立了以黄芩苷为参照峰的复方感冒颗粒HPLC特征图谱,共11个特征峰,并指认了葛根素、黄芩苷、汉黄芩素、黄芩素4个特征峰;含量测定中黄芩苷在0.046 36~0.463 6 μg范围内线性关系良好,r=0.999 8,平均回收率为100.59%,RSD为1.18%,n=6。结论:该方法操作简单,重现性良好,能有效控制复方感冒颗粒的质量。
Abstract:
Objective: To establish specific chromatogram of compound cold granules and the assay method of baicalin content. Methods: HPLC method was used to determine the specific chromatogram of compound cold granules, and Agilent TC C18 (4.6 mm×250 mm, 5μm) chromatography column was used. Acetonitrile (A) -0.2% phosphoric acid (B) solution was used as the mobile phase and gradient elution (0 minute, 10%A; 10 minutes, 14%A; 25 minutes, 18%A; 45 minutes, 25%A; 55 minutes, 28%A; 65 minutes, 42%A), with a flow rate of 1.0 mL/min, and wavelength: 266 mn, column temperature: 25℃ as well. ODS-2HYPERSIL (4.6 mm × 250 mm, 5 m) chromatography column was used in HPLC method to determine the content of baicalin in the mobile phase of methanol -0.1% phosphoric acid water (43∶57) with a wavelength of 280 nm and a flow rate of 1.0 mL/min. Results: The HPLC specific chromatogram of compound cold granules was established with baicalin as the reference peak and 11 characteristic peaks in total. Puerarin, Baicalin, Wogonin and Baicalein, were identified as four characteristic peaks; The content of baicalin was in the range of 0.04636 to 0.4636μg, and the linear relationship was good, r=0.999 8. Moreover, the average recovery rate was 100.59%, and RSD was 1.18%, n=6. Conclusion: This method is simple and reproducible, and can effectively control the quality of compound cold granules.

备注/Memo

备注/Memo:
收稿日期:2015-08-10 *基金项目:广西中医药大学附属瑞康医院基金资助项目(编号RKP201021)。 作者简介:韦瑀龙(1985—),男,硕士学位,主管中药师。研究方向:制剂质量标准研究。
更新日期/Last Update: 2017-03-15